Sgs1
Sgs1, also known as slow growth suppressor 1,[1] is a DNA helicase protein found in Saccharomyces cerevisiae. It is a homolog of the bacterial RecQ helicase. Like the other members of the RecQ helicase family, Sgs1 is important for DNA repair. In particular, Sgs1 collaborates with other proteins to repair double-strand breaks during homologous recombination in eukaryotes.[2]
Meiosis
![](http://upload.wikimedia.org/wikipedia/commons/6/6d/Homologous_Recombination.jpg)
The Sgs1(BLM) helicase is an ortholog of the human Bloom syndrome protein. It appears to be a central regulator of most of the recombination events that occur during S. cerevisiae meiosis.[3] During normal meiosis Sgs1(BLM) is responsible for directing recombination towards the alternate formation of either early non-crossover recombinants (NCOs) or Holliday junction joint molecules, the latter being subsequently resolved as crossovers (COs) (see Figure).[3] The several roles of Sgs1 in meiotic recombination were reviewed by Klein and Symington.[4] Primarily, Sgs1 displaces the strand invasion intermediate that initiates recombination, thus facilitating NCO recombination (see Homologous recombination and Bloom syndrome protein).
Sgs1 also has a role in a pathway leading to CO recombinants. Sgs1 together with EXO1 and MLH1-MLH3 heterodimer (MutL gamma) define a joint molecule resolution pathway that produces the majority of crossovers in budding yeast, and by inference, in mammals.[5]
References
- ^ Gangloff, S; et al. (December 1994). "The yeast type I topoisomerase Top3 interacts with Sgs1, a DNA helicase homolog: a potential eukaryotic reverse gyrase". Molecular and Cellular Biology. 14 (12): 8391–8398. doi:10.1128/mcb.14.12.8391. PMC 359378. PMID 7969174.
- ^ Mimitou, EP; Symington, LS (9 October 2008). "Sae2, Exo1 and Sgs1 collaborate in DNA double-strand break processing". Nature. 455 (7214): 770–774. doi:10.1038/nature07312. PMC 3818707. PMID 18806779.
- ^ a b De Muyt A, Jessop L, Kolar E, Sourirajan A, Chen J, Dayani Y, Lichten M (2012). "BLM helicase ortholog Sgs1 is a central regulator of meiotic recombination intermediate metabolism". Mol. Cell. 46 (1): 43–53. doi:10.1016/j.molcel.2012.02.020. PMC 3328772. PMID 22500736.
- ^ Klein HL, Symington LS (2012). "Sgs1--the maestro of recombination". Cell. 149 (2): 257–9. doi:10.1016/j.cell.2012.03.020. PMID 22500794.
- ^ Zakharyevich K, Tang S, Ma Y, Hunter N (2012). "Delineation of joint molecule resolution pathways in meiosis identifies a crossover-specific resolvase". Cell. 149 (2): 334–47. doi:10.1016/j.cell.2012.03.023. PMC 3377385. PMID 22500800.