Jump to content

Turbidimetry

From Wikipedia, the free encyclopedia

This is an old revision of this page, as edited by Ozzie10aaaa (talk | contribs) at 18:15, 29 July 2020 (Alter: url, isbn. | You can use this tool yourself. Report bugs here. | via #UCB_Gadget). The present address (URL) is a permanent link to this revision, which may differ significantly from the current revision.

Turbidimetry (the name being derived from turbidity) is the process of measuring the loss of intensity of transmitted light due to the scattering effect of particles suspended in it. Light is passed through a filter creating a light of known wavelength which is then passed through a cuvette containing a solution. A photoelectric cell collects the light which passes through the cuvette. A measurement is then given for the amount of absorbed light.[1]

Turbidimetry can be used in biology to find the number of cells in a solution.[2]

Immunoturbidimetry

Immunoturbidimetry is an important tool in the broad diagnostic field of clinical chemistry. It is used to determine serum proteins not detectable with classical clinical chemistry methods. Immunoturbidimetry uses the classical antigen-antibody reaction. The antigen-antibody complexes aggregate to form particles that can be optically detected by a photometer.

See also

References

  1. ^ Mary C. Haven; Gregory A. Tetrault; Jerald R. Schenken (1994). Laboratory Instrumentation. John Wiley and Sons. ISBN 0471285722.
  2. ^ D. M. Vasudevan; DM Vasudevan; S Sreekumari; Vaidyanathan Kannan (2010). Textbook of Biochemistry for Medical Students (6th ed.). Jaypee Medical Publishers. ISBN 978-9350250167.