Soft rot Enterobacteriaceae (SRE) small RNAs

Soft rot Enterobacteriaceae (SRE) (spanning the genera Erwinia, Pectobacterium, Dickeya, and Pantoea), are ubiquitous necrotrophic bacterial pathogens that infect a large number of different plant species worldwide, including economically important crops.^[1]^[2]

When they live in soil outside of their plant hosts they starve and have to adapt to this new condition. By using strand-specific RNA-seq analysis and in silico sRNA predictions 137 small RNAs candidates were identified in Pectobacterium atrosepticum under starvation conditions. This suggests that sRNAs play roles in bacterial adaptive response. The expression of 9 novel candidate sRNAs was validated by RT-PCR. Those included antisense RNAs and UTR regions.^[3]

References

^ Pritchard, Leighton; Glover, Rachel H.; Humphris, Sonia; Elphinstone, John G.; Toth, Ian K. (2015-12-17). "Genomics and taxonomy in diagnostics for food security: soft-rotting enterobacterial plant pathogens". Analytical Methods. 8 (1): 12–24. doi:10.1039/C5AY02550H. ISSN 1759-9679.
^ Charkowski, Amy O.; Lind, Jenna; Rubio-Salazar, Isael (2014-01-01). Gross, Dennis C.; Lichens-Park, Ann; Kole, Chittaranjan (eds.). Genomics of Plant-Associated Bacteria. Heidelberg: Springer Berlin Heidelberg. pp. 37–58. doi:10.1007/978-3-642-55378-3_2. ISBN 978-3-642-55377-6.
^ Kwenda, Stanford; Gorshkov, Vladimir; Ramesh, Aadi Moolam; Naidoo, Sanushka; Rubagotti, Enrico; Birch, Paul R. J.; Moleleki, Lucy N. (2016-01-12). "Discovery and profiling of small RNAs responsive to stress conditions in the plant pathogen Pectobacterium atrosepticum". BMC Genomics. 17: 47. doi:10.1186/s12864-016-2376-0. ISSN 1471-2164. PMC 4710047. PMID 26753530.{{cite journal}}: CS1 maint: unflagged free DOI (link)

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[1] Pritchard, Leighton; Glover, Rachel H.; Humphris, Sonia; Elphinstone, John G.; Toth, Ian K. (2015-12-17). "Genomics and taxonomy in diagnostics for food security: soft-rotting enterobacterial plant pathogens". Analytical Methods. 8 (1): 12–24. doi:10.1039/C5AY02550H. ISSN 1759-9679.

[2] Charkowski, Amy O.; Lind, Jenna; Rubio-Salazar, Isael (2014-01-01). Gross, Dennis C.; Lichens-Park, Ann; Kole, Chittaranjan (eds.). Genomics of Plant-Associated Bacteria. Heidelberg: Springer Berlin Heidelberg. pp. 37–58. doi:10.1007/978-3-642-55378-3_2. ISBN 978-3-642-55377-6.

[3] Kwenda, Stanford; Gorshkov, Vladimir; Ramesh, Aadi Moolam; Naidoo, Sanushka; Rubagotti, Enrico; Birch, Paul R. J.; Moleleki, Lucy N. (2016-01-12). "Discovery and profiling of small RNAs responsive to stress conditions in the plant pathogen Pectobacterium atrosepticum". BMC Genomics. 17: 47. doi:10.1186/s12864-016-2376-0. ISSN 1471-2164. PMC 4710047. PMID 26753530.{{cite journal}}: CS1 maint: unflagged free DOI (link)

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