parABS system
The parABS system is a broadly conserved molecular mechanism for plasmid partitioning and chromosome segregation in bacteria. Originally identified as a genetic element required for faithful partitioning of low-copy-number plasmids, it consists of three components: the ParA ATPase, the ParB DNA-binding protein, and the cis-acting parS sequence. The parA and parB genes are typically found in the same operon, with parS elements located within or adjacent to this operon. Collectively, these components function to ensure accurate partitioning of plasmids or whole chromosomes between bacterial daughter cells prior to cell division.[1]
Mechanism
Based on chromatin immunoprecipitation (ChIP) experiments, ParB has the ability to bind not only to high-affinity parS sites but also to adjacent nonspecific DNA, a behavior known as "spreading".[2][3][4][5] The ParB-DNA complex is thought to be translocated by a Brownian ratchet mechanism involving the ParA ATPase: ParA binds DNA nonspecifically in its ATP-bound state but much more weakly in its ADP-bound state.[6][7] The ParB-DNA complex binds to ATP-bound ParA,[8] stimulating its ATPase activity and its dissociation from DNA. In this way, the ParB-DNA complex can be translocated by chasing a receding wave.[9] This translocation mechanism has been observed by fluorescence microscopy both in vivo and more recently in vitro with purified components.[10][11][12][13]
References
- ^ Surtees, JA; Funnell, BE (2003). Plasmid and chromosome traffic control: how ParA and ParB drive partition. Vol. 56. pp. 145–80. doi:10.1016/s0070-2153(03)01010-x. ISBN 9780121531560. PMID 14584729.
{{cite book}}:|journal=ignored (help) - ^ Rodionov, O; Lobocka, M; Yarmolinsky, M (Jan 22, 1999). "Silencing of genes flanking the P1 plasmid centromere". Science. 283 (5401): 546–9. doi:10.1126/science.283.5401.546. PMID 9915704.
- ^ Murray, H; Ferreira, H; Errington, J (Sep 2006). "The bacterial chromosome segregation protein Spo0J spreads along DNA from parS nucleation sites". Molecular Microbiology. 61 (5): 1352–61. doi:10.1111/j.1365-2958.2006.05316.x. hdl:11449/701. PMID 16925562.
- ^ Breier, AM; Grossman, AD (May 2007). "Whole-genome analysis of the chromosome partitioning and sporulation protein Spo0J (ParB) reveals spreading and origin-distal sites on the Bacillus subtilis chromosome". Molecular Microbiology. 64 (3): 703–18. doi:10.1111/j.1365-2958.2007.05690.x. PMID 17462018.
- ^ Sanchez, A; Cattoni, DI; Walter, JC; Rech, J; Parmeggiani, A; Nollmann, M; Bouet, JY (2015). "Stochastic Self-Assembly of ParB Proteins Builds the Bacterial DNA Segregation Apparatus". Cell Systems. 1 (2): 163–73. doi:10.1016/j.cels.2015.07.013. PMID 27135801.
- ^ Bouet, JY; Ah-Seng, Y; Benmeradi, N; Lane, D (2007). "Polymerization of SopA partition ATPase: regulation by DNA binding and SopB". Molecular Microbiology. 63 (2): 468–81. doi:10.1111/j.1365-2958.2006.05537.x. PMID 17166176.
- ^ Castaing, JP; Bouet, JY; Lane, D (2008). "F plasmid partition depends on interaction of SopA with non-specific DNA". Molecular Microbiology. 70 (4): 1000–11. doi:10.1111/j.1365-2958.2008.06465.x. PMID 18826408.
- ^ Bouet, JY; Funnell, BE (1999). "P1 ParA interacts with the P1 partition complex at parS and an ATP-ADP switch controls ParA activities". EMBO J. 18 (5): 1415–24. doi:10.1093/emboj/18.5.1415. PMC 1171231. PMID 10064607.
- ^ Walter, JC; Dorignac, J; Lorman, V; Rech, J; Bouet, JY; Nollmann, M; Palmeri, J; Parmeggiani, A; Geniet, F (2017). "Surfing on protein waves: proteophoresis as a mechanism for bacterial genome partitioning". Physical Review Letters. 119 (28101): 028101. arXiv:1702.07372. doi:10.1103/PhysRevLett.119.028101. PMID 28753349.
- ^ Ptacin, JL; Lee, SF; Garner, EC; Toro, E; Eckart, M; Comolli, LR; Moerner, WE; Shapiro, L (Aug 2010). "A spindle-like apparatus guides bacterial chromosome segregation". Nature Cell Biology. 12 (8): 791–8. doi:10.1038/ncb2083. PMC 3205914. PMID 20657594.
- ^ Ringgaard, S; van Zon, J; Howard, M; Gerdes, K (Nov 17, 2009). "Movement and equipositioning of plasmids by ParA filament disassembly". Proceedings of the National Academy of Sciences of the United States of America. 106 (46): 19369–74. doi:10.1073/pnas.0908347106. PMC 2775997. PMID 19906997.
- ^ Hwang, LC; Vecchiarelli, AG; Han, YW; Mizuuchi, M; Harada, Y; Funnell, BE; Mizuuchi, K (May 2, 2013). "ParA-mediated plasmid partition driven by protein pattern self-organization". The EMBO Journal. 32 (9): 1238–49. doi:10.1038/emboj.2013.34. PMC 3642677. PMID 23443047.
- ^ Vecchiarelli, AG; Hwang, LC; Mizuuchi, K (Apr 9, 2013). "Cell-free study of F plasmid partition provides evidence for cargo transport by a diffusion-ratchet mechanism". Proceedings of the National Academy of Sciences of the United States of America. 110 (15): E1390–7. doi:10.1073/pnas.1302745110. PMC 3625265. PMID 23479605.
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