Search results

In materials science and molecular biology, thermostability is the ability of a substance to resist irreversible change in its chemical or physical structure...

therefore thermostable. They are used for the polymerase chain reaction and related methods for the amplification and modification of DNA. Thermostable DNA...

Taq polymerase is a thermostable DNA polymerase I named after the thermophilic eubacterial microorganism Thermus aquaticus, from which it was originally...

the high temperature (96 °C) step required to denature DNA. In 2013, a thermostable group II intron reverse transcriptase (TGIRT), GsI-IIC-MRF, from G. stearothermophilus...

their applications in DNA replication techniques, such as PCR, where thermostable enzymes are necessary for proper DNA replication. Enzyme function at...

oligonucleotides that are a complementary sequence to the target DNA region) and a thermostable DNA polymerase. In the first step of PCR, the two strands of the DNA...

Protein tertiary structure is the three-dimensional shape of a protein. The tertiary structure will have a single polypeptide chain "backbone" with one...

motility or remain immotile. T. aquaticus has become famous as a source of thermostable enzymes, particularly the Taq DNA polymerase, as described below. Studies...

enzymes require a metal ion co-factor. Thermostable proteins are often more useful than their non-thermostable counterparts, e.g., DNA polymerase in the...

thermal cycles (94 °C/80 °C) or 16 hours of cycling.10 This exceptional thermostability permits extremely high hybridization stringency and ligation specificity...

where it functions to copy the organism's DNA during cell division (thermostable DNA polymerase). In the laboratory setting, Pfu is used to amplify DNA...

an amplification process that differs from PCR in that it involves a thermostable ligase to join two probes or other molecules together which can then...

biotechnological potential due to their ability to produce industrial-relevant thermostable enzymes, in particular for the degradation of plant biomass. Sulfolobus...

Poikilotherm Homeothermy Heterothermy Stenotherm Eurytherm Thermolabile Thermostability Gigantothermy Kleptothermy Bradymetabolism Tachymetabolism Thermogenesis...

polymerizes partner strands in each direction from these primers using a thermostable DNA polymerase. Repeating this process through multiple cycles amplifies...

these harsh conditions. These enzymes have found many uses. For example, thermostable DNA polymerases, such as the Pfu DNA polymerase from Pyrococcus furiosus...

Bacillus licheniformis or Bacillus subtilis (strain MN-385), which are more thermostable than the originally used enzymes. Starting in 1982, pullulanases from...

Food and Drug Administration. Wilding, Kristen (25 November 2019). "Thermostable lyoprotectant-enhanced cell-free protein synthesis for on-demand endotoxin-free...

the polymerase chain reaction (PCR) process, before being replaced by thermostable DNA polymerases such as Taq polymerase. Just as the 5' → 3' exonuclease...

Poikilotherm Homeothermy Heterothermy Stenotherm Eurytherm Thermolabile Thermostability Gigantothermy Kleptothermy Bradymetabolism Tachymetabolism Thermogenesis...