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Asio is a genus of owls.
Asio or ASIO may also refer to:
- asio C++ library, a programming library for asynchronous I/O
- Audio Stream Input/Output, computer sound card driver protocol for low-latency digital audio
- Australian Security Intelligence Organisation
- "ASIO", a song by Redgum from Frontline
- "ASIO" a song by Benny Cristo