Acid fuchsin

Acid fuchsin
Names
	IUPAC name Disodium 2-amino-5-[(Z)-(4-amino-3-sulfonatophenyl)(4-iminio-3-sulfonato-2,5-cyclohexadien-1-ylidene)methyl]-3-methylbenzenesulfonate
Identifiers
CAS Number	3244-88-0;
3D model (JSmol)	Interactive image;
ChEBI	CHEBI:87052 ;
ChemSpider	16736101;
ECHA InfoCard	100.019.833
CompTox Dashboard (EPA)	DTXSID8044418 ;
	InChI InChI=1S/C20H19N3O9S3.2Na/c1-10-6-13(9-18(20(10)23)35(30,31)32)19(11-2-4-14(21)16(7-11)33(24,25)26)12-3-5-15(22)17(8-12)34(27,28)29;;/h2-9,21H,22-23H2,1H3,(H,24,25,26)(H,27,28,29)(H,30,31,32);;/q;2+1/p-2/b19-11-,21-14?;; Key: RZUBARUFLYGOGC-MTHOTQAESA-L; InChI=1/C20H19N3O9S3.2Na/c1-10-6-13(9-18(20(10)23)35(30,31)32)19(11-2-4-14(21)16(7-11)33(24,25)26)12-3-5-15(22)17(8-12)34(27,28)29;;/h2-9,21H,22-23H2,1H3,(H,24,25,26)(H,27,28,29)(H,30,31,32);;/q;2+1/p-2/b19-11-,21-14?;; Key: RZUBARUFLYGOGC-LOQRZINWBG;
	SMILES CC1=CC(=CC(=C1N)S(=O)(=O)[O-])/C(=C\2/C=CC(=[NH2+])C(=C2)S(=O)(=O)[O-])/C3=CC(=C(C=C3)N)S(=O)(=O)[O-].[Na+].[Na+];
Properties
Chemical formula	C20H17N3Na2O9S3
Molar mass	585.53 g·mol−1
	Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). Infobox references

Acid fuchsin is an acidic dye that has wide use in histology. It is one of the dyes used in Masson's trichrome stain. This method is commonly used to stain cytoplasm and nuclei of tissue sections in the histology laboratory in order to distinguish muscle from collagen. The muscle stains red with the acid fuchsin, and the collagen is stained green or blue with light green SF yellowish or methyl blue.

Formula:

1 g of acid fuchsin
1 mL of glacial acetic acid (HOAc)
100 mL of distilled water

References

Jocelyn H. Bruce-Gregorios, M.D.: Histopathologic Techniques, JMC Press Inc., Quezon City, Philippines, 1974. ISBN 971-11-0853-4

See also

References