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Bacterial murein precursor exporter

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The bacterial murein precursor exporter (MPE) family (TC# 2.A.103) is a member of the cation diffusion facilitator (CDF) superfamily of membrane transporters. Members of the MPE family are found in a large variety of Gram-negative and Gram-positive bacteria and facilitate the translocation of lipid-linked murein (aka peptidoglycan) precursors. A representative list of proteins belonging to the MPE family can be found in the Transporter Classification Database.[1]

Structure

Members of the MPE family consist of 370-420 amino acyl residues with 9 (RodA; TC# 2.A.103.1.2) or 10 (FtsW; TC# 2.A.103.1.1) putative transmembrane α-helical spanners. Experimental evidence for a 10 TMS model has been reported for FtsW of Streptococcus pneumoniae.[2] The S. pneumoniae protein has both its N- and C-termini in the cytoplasm, a large (~ 60 residue) cytoplasmic domain between TMSs 4 and 5, and a large (~ 80 residue) extracytoplasmic loop between TMSs 7 and 8.

Function

Bacterial cell growth necessitates synthesis of peptidoglycan. Assembly of peptidoglycan is a multistep process starting in the cytoplasm and ending in the exterior cell surface. The intracellular part of the pathway results in the production of the membrane-anchored cell wall precursor, Lipid II. After synthesis, this lipid intermediate is translocated across the cell membrane. The translocation (flipping) step of Lipid II requires a specific protein (flippase). Mohammadi et al. (2011) showed that the integral membrane protein FtsW (TC# 2.A.103.1.1,4-7), an essential protein for cell division, is a transporter of the lipid-linked peptidoglycan precursors across the cytoplasmic membrane. Using E. coli membrane vesicles, they found that transport of Lipid II requires the presence of FtsW, and purified FtsW induced the transbilayer movement of Lipid II in model membranes.[3]

The best-characterized members of the family are the FtsW cell division protein, the RodA rod shape determining protein (both of E. coli; TC# 2.A.103.1.2) and the SpoVE protein of B. subtilis (TC# 2.A.103.1.3).[4][5][6][7] They have been shown to function in the translocation (export) of lipid-linked murein precursors such as NAG-NAM-pentapeptide pyrophosphoryl undecaprenol (lipid II).[8] They interact with murein synthases as well as two transpeptidases (PBP2 and PBP3). In Gram-negative bacteria the ftsW gene is physically linked to murG (TC# 9.B.146), which is responsible for the final cytoplasmic step in the synthesis of lipid II before it is flipped to the periplasmic side of the membrane. They may therefore be part of a tunneling device directing the flow of murein precursors to the membrane enzymes that insert the precursors into the preexisting murein sacculus.

Transport reaction

The following reaction is catalyzed by the proteins of the MPE family.[1]

Lipid-linked murein precursor (in) → Lipid-linked murein precursor (out)

See also

References

  1. ^ a b Saier, MH Jr. "2.A.103 The Bacterial Murein Precursor Exporter (MPE) Family". Transporter Classification Database. Saier Lab Bioinformatics Group / SDSC.
  2. ^ Gérard, Philippe; Vernet, Thierry; Zapun, André (2002-04-01). "Membrane topology of the Streptococcus pneumoniae FtsW division protein". Journal of Bacteriology. 184 (7): 1925–1931. doi:10.1128/jb.184.7.1925-1931.2002. ISSN 0021-9193. PMC 134934. PMID 11889099.
  3. ^ Mohammadi, Tamimount; van Dam, Vincent; Sijbrandi, Robert; Vernet, Thierry; Zapun, André; Bouhss, Ahmed; Diepeveen-de Bruin, Marlies; Nguyen-Distèche, Martine; de Kruijff, Ben (2011-04-20). "Identification of FtsW as a transporter of lipid-linked cell wall precursors across the membrane". The EMBO journal. 30 (8): 1425–1432. doi:10.1038/emboj.2011.61. ISSN 1460-2075. PMC 3102273. PMID 21386816.
  4. ^ Boyle, D. S.; Khattar, M. M.; Addinall, S. G.; Lutkenhaus, J.; Donachie, W. D. (1997-06-01). "ftsW is an essential cell-division gene in Escherichia coli". Molecular Microbiology. 24 (6): 1263–1273. doi:10.1046/j.1365-2958.1997.4091773.x. ISSN 0950-382X. PMID 9218774.
  5. ^ Errington, J (2003). "The Bacterial Actin Cytoskeleton: Actin-like proteins in bacteria form a cytoskeleton that helps to determine cell shape, much as happens in eukaryotic cells" (PDF). ASM News. 69 (12).
  6. ^ Matsuzawa, H.; Asoh, S.; Kunai, K.; Muraiso, K.; Takasuga, A.; Ohta, T. (1989-01-01). "Nucleotide sequence of the rodA gene, responsible for the rod shape of Escherichia coli: rodA and the pbpA gene, encoding penicillin-binding protein 2, constitute the rodA operon". Journal of Bacteriology. 171 (1): 558–560. ISSN 0021-9193. PMC 209621. PMID 2644207.
  7. ^ Sato, T.; Theeragool, G.; Yamamoto, T.; Okamoto, M.; Kobayashi, Y. (1990-07-11). "Revised nucleotide sequence of the sporulation gene spoVE from Bacillus subtilis". Nucleic Acids Research. 18 (13): 4021. doi:10.1093/nar/18.13.4021. ISSN 0305-1048. PMC 331137. PMID 2115675.
  8. ^ Mohammadi, Tamimount; Sijbrandi, Robert; Lutters, Mandy; Verheul, Jolanda; Martin, Nathaniel I.; den Blaauwen, Tanneke; de Kruijff, Ben; Breukink, Eefjan (2014-05-23). "Specificity of the transport of lipid II by FtsW in Escherichia coli". The Journal of Biological Chemistry. 289 (21): 14707–14718. doi:10.1074/jbc.M114.557371. ISSN 1083-351X. PMC 4031526. PMID 24711460.{{cite journal}}: CS1 maint: unflagged free DOI (link)
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