Bismarck brown Y

Bismarck brown Y
Names
	Other names Bismarck brown ; Manchester brown ; Phenylene brown ; Basic Brown 1 ; C.I. 21000; Vesuvine BA
Identifiers
CAS Number	8005-77-4 ;
3D model (JSmol)	Interactive image; Interactive image;
ChemSpider	13374 ;
ECHA InfoCard	100.030.273
PubChem CID	13981;
CompTox Dashboard (EPA)	DTXSID5024558 ;
	InChI InChI=1S/C18H18N8/c19-11-4-6-17(15(21)8-11)25-23-13-2-1-3-14(10-13)24-26-18-7-5-12(20)9-16(18)22/h1-10H,19-22H2/b25-23+,26-24+ Key: BDFZFGDTHFGWRQ-OGGGYYITSA-N ; InChI=1/C18H18N8/c19-11-4-6-17(15(21)8-11)25-23-13-2-1-3-14(10-13)24-26-18-7-5-12(20)9-16(18)22/h1-10H,19-22H2/b25-23+,26-24+ Key: BDFZFGDTHFGWRQ-OGGGYYITBR;
	SMILES CC1=C(C=C(C=C1)N=NC2=C(C=C(C(=C2)C)N)N)N=NC3=C(C=C(C(=C3)C)N)N; N(=N/c1ccc(cc1N)N)\c3cccc(/N=N/c2ccc(N)cc2N)c3;
Properties
Chemical formula	C18H18N8·2HCl
Molar mass	419.31 g/mol
	Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). verify (what is ?) Infobox references

Bismarck brown Y is a diazo dye. It is used in histology for staining tissues. It stains acid mucins to yellow color. It can be used with live cells. It is also used to stain cartilage in bone specimens, as one of Kasten's Schiff-type reagents in the periodic acid-Schiff stain to stain cellulose, and in Feulgen stain to stain DNA.

It was more common in the past; today it is partially replaced by other stains.

Bismarck brown Y is a constituent of Papanicolaou stains.

It can also be used as a counterstain for Victoria blue R for staining of acid-fast microorganisms.

References

Lillie, RD (1977) Conn's Biological Stains, 9th ed. Baltimore: Williams & Wilkins, p. 145-146.