EDANS
Appearance
![]() | |
Names | |
---|---|
IUPAC name
5-((2-aminoethyl)amino)naphthalene-1-sulfonic acid
| |
Other names
EDANS
| |
Identifiers | |
3D model (JSmol)
|
|
ChemSpider | |
ECHA InfoCard | 100.051.415 |
PubChem CID
|
|
CompTox Dashboard (EPA)
|
|
| |
| |
Properties | |
C12H14N2O3S | |
Molar mass | 266.32 |
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
|
EDANS (5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid) is a donor for FRET-based nucleic acid probes and protease substrates. EDANS is often paired with DABCYL or DABSYL. The combination can be used in enzyme assays. When the two compounds are in close proximity, most of the energy emitted from EDANS will be quenched by DABCYL. However, if the compounds are separated (for example, by substrate cleavage) EDANS will fluoresce, giving an indication of enzyme presence.[1]
See Also
References
- ^ Guo, Jiubiao; et al. (30 November 2014). "Spectral properties of EDANS-Dabcyl pair [45] and the flowchart of the experimental design". PLOS One. 9 (12). doi:10.1371/journal.pone.0114124.g001.
{{cite journal}}
: CS1 maint: unflagged free DOI (link)