Expression cassette

An expression cassette is a distinct component of vector DNA consisting of a gene and regulatory sequence to be expressed by a transfected cell.^[1] In each successful transformation, the expression cassette directs the cell's machinery to make RNA and protein(s). Some expression cassettes are designed for modular cloning of protein-encoding sequences so that the same cassette can easily be altered to make different proteins.^[2]

An expression cassette is composed of one or more genes and the sequences controlling their expression.^[3] An expression cassette comprises three components: a promoter sequence, an open reading frame, and a 3' untranslated region that, in eukaryotes, usually contains a polyadenylation site.^[4]

Different expression cassettes can be transfected into different organisms including bacteria, yeast, plants, and mammalian cells as long as the correct regulatory sequences are used.^[5]^[6]

References

^ Papadakis, E.D.; et al. (2004). "Promoters and Control Elements: Designing Expression Cassettes for Gene Therapy". Current Gene Therapy (4): 89–113.
^ Bertrand, Edouard; et al. (1997). "The expression cassette determines the functional activity of ribozymes in mammalian cells by controlling their intracellular localization" (PDF). RNA. 3: 75–88. PMC 1369464. PMID 8990401.
^ Vickers, Claudia E (December 2013). "Dual gene expression cassette vectors with antibiotic selection markers for engineering in Saccharomyces cerevisiae". Microbial Cell Factories. 12 (96). doi:10.1186/1475-2859-12-96.{{cite journal}}: CS1 maint: unflagged free DOI (link)
^ Gray, Steven James; et al. (January 2015). "Viral Expression Cassette Elements to Enhance Transgene Target Specificity and Expression in Gene Therapy". Discov Med. 19 (102): 49–57. ISSN 1539-6509. PMC 4505817. PMID 25636961.
^ Azhakanandam, K.; et al. (2015). Recent Advancements in Gene Expression and Enabling Technologies in Crop Plants. Springer. p. 41-52. doi:10.1007/978-1-4939-2202-4_2.
^ Chow, Yu-Hua; et al. (December 1997). "Development of an epithelium-specific expression cassette with human DNA regulatory elements for transgene expression in lung airways" (PDF). Proceedings of the National Academy of Sciences. 94: 14695–14700. doi:10.1073/pnas.94.26.14695. PMC 25096.

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[1] Papadakis, E.D.; et al. (2004). "Promoters and Control Elements: Designing Expression Cassettes for Gene Therapy". Current Gene Therapy (4): 89–113.

[2] Bertrand, Edouard; et al. (1997). "The expression cassette determines the functional activity of ribozymes in mammalian cells by controlling their intracellular localization" (PDF). RNA. 3: 75–88. PMC 1369464. PMID 8990401.

[3] Vickers, Claudia E (December 2013). "Dual gene expression cassette vectors with antibiotic selection markers for engineering in Saccharomyces cerevisiae". Microbial Cell Factories. 12 (96). doi:10.1186/1475-2859-12-96.{{cite journal}}: CS1 maint: unflagged free DOI (link)

[4] Gray, Steven James; et al. (January 2015). "Viral Expression Cassette Elements to Enhance Transgene Target Specificity and Expression in Gene Therapy". Discov Med. 19 (102): 49–57. ISSN 1539-6509. PMC 4505817. PMID 25636961.

[5] Azhakanandam, K.; et al. (2015). Recent Advancements in Gene Expression and Enabling Technologies in Crop Plants. Springer. p. 41-52. doi:10.1007/978-1-4939-2202-4_2.

[6] Chow, Yu-Hua; et al. (December 1997). "Development of an epithelium-specific expression cassette with human DNA regulatory elements for transgene expression in lung airways" (PDF). Proceedings of the National Academy of Sciences. 94: 14695–14700. doi:10.1073/pnas.94.26.14695. PMC 25096.

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See also

References