English: "Figure 8. PLD1 regulates choline to alleviate Cd-induced mitochondrial damage. Elisa measured the amount of TFAM in mice (A) and AML12 cells (H). Levels of PLD1 were determined using Western blotting in mice (B) and AML12 cells (I). Elisa measures the amount of choline in mice (C). ATP content (D) and mitochondrial respiratory complex IV (E) and V (F) activity were detected. MitoSOX detects mitochondrial ROS (G). Based on treatment with the PLD1 OE plasmid for 24 h, AML12 cells were treated with or without Cd for 12 h, Elisa measures the amount of choline in AML12 (J). ATP content (K) and mitochondrial respiratory complex IV (L) and V (M) activity were detected. MitoSOX detects mitochondrial ROS (N). Results are shown as the mean ± SD (n = 3). Compared with the control group, * p < 0.05, ** p < 0.01. Compared with the Cd group, ns p > 0.05, # p < 0.05, ## p < 0.01." Sun et al. 2024