(A) Comparison of T1-weighted 3D FSPGR (fast-spoiled gradient-recalled echo) and (B) T2-weighted 3D FIESTA MRI pulse sequences acquired on the 3T scanner (slice matrix = 512×512×236, slice thickness = 900 µm, resolution = 683 µm3, averages = 2) in the smooth hammerhead, Sphyrna lewini (SIO 64-528, SL 104 cm). Additional parameters for the T1-weighted 3D Fast-Spoiled Gradient Echo (FSPGR) pulse sequence include, FA = 35°, TR = 9.86 ms, TE = 4.112 ms, and for the T2-weighted 3D FIESTA pulse sequence, FA = 40°, TR = 4.456 ms, TE = 2.1 ms. (C) The red bream, Beryx decadactylus (SIO 85-77; SL 289 mm), was initially imaged with a T1-weighted FSPGR pulse sequence on the 3T scanner prior to rehydration, and (D) re-imaged following rehydration resulting in an enhanced image quality. Scan parameters: FA = 30°, TR = 11.904 ms, TE = 3.932 ms, slice matrix = 512×512×236, slice thickness = 600 µm, resolution = 527 µm3, averages = 3. (E) The fantail filefish, Pervagor spilosoma (SIO 53-539; SL 74 mm) was initially imaged on the 7T scanner using a T1-weighted FLASH pulse sequence without exposure to contrast agent, ProHance. (F) It was subsequently reimaged following exposure to 2.5 mM ProHance, resulting in a significantly brighter MR signal and enhanced visual contrast among tissues. Scan parameters: FA = 15°, TR = 25.875 ms, TE = 12.853 ms, slice matrix = 350×1000×420, slice thickness = 100 µm, resolution = 100 µm3, averages = 8.