English: Illustration of how cold shock affects the supercoiling state of the DNA, by blocking the activity of Gyrase. The signs ‘ − ’ and ‘+’ represent negative and positive supercoiling, respectively. Created with BioRender.com. Also shown is a stochastic model of gene expression during cold shock as a function of the global DNA supercoiling state. The transition from ON to OFF of the promoter (P) causes the locking of transcription (i.e. RNA production). When ON, the promoter can produce RNA, from which proteins can be produced. RNA and proteins are always subject to degradation or dilution due to cell division.
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