Fluo-4
Appearance
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Names | |
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IUPAC name
Fluo-4
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Systematic IUPAC name
2,2'-((2-(2-(2-(bis(carboxymethyl)amino)-5-(2,7-difluoro-6-hydroxy-3-oxo-3H-xanthen-9-yl)phenoxy)ethoxy)-4-methylphenyl)azanediyl)diacetic acid | |
Identifiers | |
3D model (JSmol)
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ChEBI | |
MeSH | Fluo-4 |
CompTox Dashboard (EPA)
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Properties | |
C36H30F2N2O13 | |
Molar mass | 736.634 g·mol−1 |
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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Fluo-4 is used to measure calcium (Ca2+) concentrations inside living cells, and is often used for high-throughput screening of receptor ligands and calcium permeable ion channels.
The green-fluorescent calcium indicator, Fluo-4, is an improved version of the calcium indicator, Fluo-3. It is commonly used as the non-fluorescent acetoxymethyl ester (Fluo-4 AM) which is cleaved inside the cell to give the free, fluorescent Fluo-4. It loads faster, is brighter at equivalent concentrations and is well-excited by the 488 nm line of the argon-ion laser which is often used in biological research laboratories.[1] The sole distributor and owner of the patents is Invitrogen which acquired the original inventor Molecular Probes.
References
- ^ Gee KR, Brown KA, Chen WN, Bishop-Stewart J, Gray D, Johnson I (February 2000). "Chemical and physiological characterization of fluo-4 Ca(2+)-indicator dyes". Cell Calcium. 27 (2): 97–106. doi:10.1054/ceca.1999.0095. PMID 10756976.
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