Geobacter

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Geobacter
Scientific classification
Kingdom:
Bacteria
Phylum:
Proteobacteria
Class:
Deltaproteobacteria
Order:
Desulfuromonadales
Family:
Geobacteraceae
Genus:
Geobacter
Species

G. anodireducens[1]
G. argillaceus
G. bemidjiensis
G. bremensis
G. chapellei
G. daltonii
G. grbiciae
G. hydrogenophilus
G. lovleyi[1]
G. luticola[1]
G. metallireducens
G. pelophilus
G. pickeringii
G. psychrophilus
G. soli[1]
G. sulfurreducens
G. thiogenes
G. toluenoxydans
G. uraniireducens[1]

Geobacter is a genus of Proteobacteria. Geobacter species are anaerobic respiration bacterial species which have capabilities that make them useful in bioremediation. Geobacter was found to be the first organism with the ability to oxidize organic compounds and metals, including iron, radioactive metals, and petroleum compounds into environmentally benign carbon dioxide while using iron oxide or other available metals as electron acceptors.[2] Geobacter species are also found to be able to respire upon a graphite electrode.[3] They have been found in anaerobic conditions in soils and aquatic sediment.[4]

History

Geobacter metallireducens was first isolated by Derek Lovley in 1987 in sand sediment from the Potomac River in Washington D.C. The first strain was deemed strain GS-15.[4]

Applications

Geobacter's ability to consume oil-based pollutants and radioactive material with carbon dioxide as waste byproduct has been used in environmental clean-up for underground petroleum spills and for the precipitation of uranium out of groundwater.[5][6] Geobacter metabolize the material by creating electrically conductive pili between itself and the food material.[7]

Multiple Geobacter species cooperate in metabolizing a mixture of chemicals that neither could process alone. Provided with ethanol and sodium fumarate, G. metallireducens broke down the ethanol, generating an excess of electrons that were passed to G. sulfurreducens via "nanowires" grown between them, enabling G. sulfurreducens to break down the fumarate ions.[8] The nanowires are made of proteins with metal-like conductivity.[9]

Microgravity

Geobacter has been tested in microgravity and this does not make a substantial difference.

Biodegradation and bioremediation

Microbial biodegradation of recalcitrant organic pollutants is of great environmental significance and involves intriguing novel biochemical reactions. In particular, hydrocarbons and halogenated compounds have long been doubted to be anaerobically degradable, but the isolation of hitherto unknown anaerobic hydrocarbon-degrading and reductively dehalogenating bacteria documented these processes in nature. Novel biochemical reactions were discovered, enabling the respective metabolic pathways, but progress in the molecular understanding of these bacteria was slowed by the absence of genetic systems for most of them. However, several complete genome sequences later became available for such bacteria. The genome of the hydrocarbon degrading and iron-reducing species G. metallireducens (accession nr. NC_007517) was determined in 2008. The genome revealed the presence of genes for reductive dehalogenases, suggesting a wide dehalogenating spectrum. Moreover, genome sequences provided insights into the evolution of reductive dehalogenation and differing strategies for niche adaptation.[10]

Geobacter species are often the predominant organisms when extracellular electron transfer is an important bioremediation process in subsurface environments. Therefore, a systems biology approach to understanding and optimizing bioremediation with Geobacter species has been initiated with the ultimate goal of developing in silico models that can predict the growth and metabolism of Geobacter species under a diversity of subsurface conditions. The genomes of multiple Geobacter species have been sequenced. Detailed functional genomic/physiological studies on one species, G. sulfurreducens was conducted. Genome-based models of several Geobacter species that are able to predict physiological responses under different environmental conditions are available. Quantitative analysis of gene transcript levels during in situ uranium bioremediation demonstrated that it is possible to track in situ rates of metabolism and the in situ metabolic state of Geobacter in the subsurface.[11]

Popular culture

Geobacter is used as a plot device in the first episode of the third season of ReGenesis. Geobacter has become an icon for teaching about microbial electrogenesis and microbial fuel cells and has appeared in educational kits that are available for students and hobbyists.[12] The genus even has its own plush toy[13] Geobacter is also used to generate electricity via electrode grid in Amazon,Peru. It is also referenced in the James Rollins novel, The Seventh Plague.

See also

References

  1. ^ a b c d e LPSN bacterio.net
  2. ^ Childers, Susan (2002). "Geobacter metallireducens accesses insoluble Fe (III) oxide by chemotaxis". Nature. 416: 767–769. doi:10.1038/416767a. PMID 11961561. Retrieved 21 August 2015.
  3. ^ Bond, Daniel (Mar 2003). "Electricity Production by Geobacter sulfurreducens Attached to Electrodes". Applied and Environmental Microbiology. 69 (3): 1548–1555. doi:10.1128/AEM.69.3.1548-1555.2003. PMC 150094. PMID 12620842. Retrieved 26 August 2015.
  4. ^ a b Lovley DR, Stolz JF, Nord GL, Phillips EJP (1987). "Anaerobic Production of Magnetite by a Dissimilatory Iron-Reducing Microorganism" (PDF). Nature. 350 (6145): 252–254. doi:10.1038/330252a0.
  5. ^ Anderson RT, Vrionis HA, Ortiz-Bernad I, Resch CT, Long PE, Dayvault R, Karp K, Marutzky S, Metzler DR, Peacock A, White DC, Lowe M, Lovley DR (2003). "Stimulating the in situ activity of Geobacter species to remove uranium from the groundwater of a uranium-contaminated aquifer". Applied and Environmental Microbiology. 69 (10): 5884–91. doi:10.1128/aem.69.10.5884-5891.2003. PMC 201226. PMID 14532040.
  6. ^ Cologgi, Dena (2014). "Enhanced uranium immobilization and reduction by Geobacter sulfurreducens biofilms". Applied and Environmental Microbiology. 80 (21): 6638–6646. doi:10.1128/AEM.02289-14. PMC 4249037. PMID 25128347.
  7. ^ "Experiment and theory unite at last in debate over microbial nanowires". Phys.org. Retrieved 5 January 2016.
  8. ^ Williams, Caroline (2011). "Who are you calling simple?". New Scientist. 211 (2821): 38–41. doi:10.1016/S0262-4079(11)61709-0.
  9. ^ Malvankar, Nikhil; Vargas, Madeline; Nevin, Kelly; Tremblay, Pier-Luc; Evans-Lutterodt, Kenneth; Nykypanchuk, Dmytro; Martz, Eric; Tuominen, Mark T; Lovley, Derek R (2015). "Structural Basis for Metallic-Like Conductivity in Microbial Nanowires". mBio. 6 (2).
  10. ^ Heider J, Rabus R (2008). "Genomic Insights in the Anaerobic Biodegradation of Organic Pollutants". Microbial Biodegradation: Genomics and Molecular Biology. Caister Academic Press. ISBN 978-1-904455-17-2. {{cite book}}: External link in |chapterurl= (help); Unknown parameter |chapterurl= ignored (|chapter-url= suggested) (help)
  11. ^ Diaz E, ed. (2008). Microbial Biodegradation: Genomics and Molecular Biology (1st ed.). Caister Academic Press. ISBN 978-1-904455-17-2.
  12. ^ MudWatt Science Kit
  13. ^ Magical Microbes. "Geo Plush Toy". Magical Microbes.

External links

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