J558L cells

J558L cells are mouse B myeloma cells derived from BALB/c strain isolated by M. Bruggeman and M.S. Neuberger in University of Cambridge.^[1]^[2] It is a stable B cell line used to express antibodies. They are a suspension cell line and are best cultured in Eagle's minimal essential medium with 10% Fetal bovine serum, 0.01 units/ml of penicillin and 0.1 mg/ml streptomycin incubated in 37 °C + 5% CO₂ + 90% relative humidity with a cell density of 5x10⁵ cell per 15 ml.

Uses

This cell line is usually used to express antibodies since they can result in antibody titers up to 1 mg under optimum conditions.

References

^ Oi, VT; Morrison, SL; Herzenberg, LA; Berg, P (1983). "Immunoglobulin gene expression in transformed lymphoid cells". Proc. Natl. Acad. Sci. U.S.A. 80: 825–9. doi:10.1073/pnas.80.3.825. PMC 393473. PMID 6402777.
^ http://www.hpacultures.org.uk/products/celllines/generalcell/detail.jsp?refId=88032902&collection=ecacc_gc

External links

Cellosaurus entry for J558L

[1] Oi, VT; Morrison, SL; Herzenberg, LA; Berg, P (1983). "Immunoglobulin gene expression in transformed lymphoid cells". Proc. Natl. Acad. Sci. U.S.A. 80: 825–9. doi:10.1073/pnas.80.3.825. PMC 393473. PMID 6402777.

[2] ttp://www.hpacultures.org.uk/products/celllines/generalcell/detail.jsp?refId=88032902&collection=ecacc_gc

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