Pleurotolysin
| Pleurotolysin B | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||||
| Symbol | plyB | ||||||||||
| Pfam | PF01823 | ||||||||||
| InterPro | IPR020864 | ||||||||||
| PROSITE | PS51412 | ||||||||||
| |||||||||||
Pleurotolysin (TC# 1.C.97.1.1), a sphingomyelin-specific cytolysin consisting of A (17 kDa; Q8X1M9) and B (59 kDa; Q5W9E8) components from the basidiomycete Pleurotus ostreatus, assembles into a transmembrane pore complex.[1] The Pleurotolysin Pore-Forming (Pleurotolysin) Family (TC# 1.C.97) is a family of pore forming proteins belonging to the MACPF superfamily. A representative list of members of the Pleurotolysin family can be found in the Transporter Classification Database.
Function
Proteins with membrane-attack complex/perforin (MACPF) domains have a variety of biological roles, including defense and attack, organismal development, and cell adhesion and signaling.[2] The distribution of these proteins in fungi appears to be restricted to some Pezizomycotina and Basidiomycota species only, in correlation with the aegerolysins (PF06355). These two protein groups coincide in only a few species, and they operate as cytolytic bi-component pore-forming agents.[2] Representative proteins include pleurotolysin B, which has a MACPF domain, the aegerolysin-like protein pleurotolysin A, and the very similar ostreolysin A (TC# 1.C.97.3.2) that has been purified from oyster mushroom (Pleurotus ostreatus). These act in concert to perforate natural and artificial lipid membranes with high cholesterol and sphingomyelin contents. The complex has a 13-meric rosette-like structure with a central lumen that is ~ 4-5 nm in diameter. The opened transmembrane pore is non-selectively permeable to ions and smaller neutral solutes, and is a cause of cytolysis of a colloid-osmotic type.
Research
Sakurai et al. 2004 cloned complementary and genomic DNAs encoding pleurotolysin, and studied pore-forming properties of recombinant proteins. Recombinant pleurotolysin A lacking the first methionine was purified as a 17-kDa protein with sphingomyelin-binding activity. The cDNA for pleurotolysin B encoded a precursor consisting of 523 amino acyl residues, of which 48 N-terminal amino acyl residues were absent in natural pleurotolysin B. Mature and precursor forms of pleurotolysin B were expressed as insoluble 59- and 63-kDa proteins, respectively. Although neither recombinant pleurotolysin A nor B alone was hemolytically active at higher concentrations of up to 100 mg/ml, they cooperatively assembled into a membrane pore complex on human erythrocytes and lysed the cell.[3]
Tomita et al. 2004 purified pleurotolysin, a sphingomyelin-specific two-component cytolysin from the basidiocarps of Pleurotus ostreatus and studied pore-formation. Pleurotolysin caused leakage of potassium ions from human erythrocytes and formed membrane pores with a functional diameter of 4-5 nm. Pleurotolysin-induced lysis of human erythrocytes was inhibited by the addition of sphingomyelin-cholesterol liposomes to the extracellular space. Pleurotolysin A specifically bound to sphingomyelin-cholesterol liposomes and caused leakage of the internal carboxyfluorescein in concert with pleurotolysin B. Pleurotolysin A and B bound to human erythrocytes in this sequence and assembled into an SDS-stable, 700-kDa complex. Ring-shaped structures with outer and inner diameters of 14 and 7 nm, respectively, were isolated from the solubilized erythrocyte membranes.[1]
Crystal Structures
There are several crystal structures available for Pleurotolysin A/B in RCSB. Some of these structures include:
4OEJ, 4OE8, 4V2T, 4V3A, 4V3M, 4V3N, 4OEB, 4V2T, 4V3A
Homologues
In this TC family, both constituents of pleurotolysin and ostreolysin (A and B) are included under TC#s 1.C.97.1.1 and 1.C.97.1.2, respectively. However, homologues of Pleurotolysin B are found under TC#s 1.C.97.1.3 - 1.C.97.1.9 while homologues of Pleurotolysin A are found under TC#s 1.C.97.2.1 - 1.C.97.2.4 and TC#s 1.C.97.3.1 - 1.C.97.3.8. Pleurotolysins A are not homologous to Pleurotolysins B. While some homologues depend on the presence of both constituents for pore formation, as noted for both pleurotolysin and ostreolysin, some homologues of both A and B can form pores without the other. While Pleurotolysin B is in the MACPF superfamily (TC# 1.C.39) while Pleurotolysin A is in the Aegerolysin superfamily.
Erylysin
Another two-component hemolysin, erylysin A and B (EryA and EryB; TC# 1.C.97.1.2), was isolated from an edible mushroom, Pleurotus eryngii.[4] Hemolytic activity was exhibited only by the EryA and EryB mixture.
Aegerolysin
While Pleurotolysin B is in the MACPF superfamily (TC# 1.C.39), Pleurotolysin A is in the Aegerolysin superfamily. Several members of the Aegerolysin family have been used as tools to detect and visualize ceramide phosphoethanolamine, a major sphingolipid in invertebrates but not in animals.[5] It may be distantly related to members of the Equinatoxin Family (TC# 1.C.38).
The aegerolysin family consists of several bacterial and eukaryotic aegerolysin-like proteins. It has been found that aegerolysin and ostreolysin are expressed during formation of primordia and fruiting bodies and possibly play a role in the initial phase of fungal fruiting. The bacterial members of this family are expressed during sporulation. Ostreolysin is cytolytic to various erythrocytes and tumor cells because of pore formation.[6][7][8] Several members of the Aegerolysin family have been used as tools to detect and visualize ceramide phosphoethanolamine, a major sphingolipid in invertebrates but not in animals.[5] It may be distantly related to members of the Equinatoxin Family (TC# 1.C.38).
References
- ^ a b Tomita, Toshio; Noguchi, Kayoko; Mimuro, Hitomi; Ukaji, Fumio; Ito, Kiyoshi; Sugawara-Tomita, Noriko; Hashimoto, Yohichi (2004-06-25). "Pleurotolysin, a novel sphingomyelin-specific two-component cytolysin from the edible mushroom Pleurotus ostreatus, assembles into a transmembrane pore complex". The Journal of Biological Chemistry. 279 (26): 26975–26982. doi:10.1074/jbc.M402676200. ISSN 0021-9258. PMID 15084605.
{{cite journal}}: CS1 maint: unflagged free DOI (link) - ^ a b Ota, Katja; Butala, Matej; Viero, Gabriella; Dalla Serra, Mauro; Sepčić, Kristina; Maček, Peter (2014-01-01). "Fungal MACPF-like proteins and aegerolysins: bi-component pore-forming proteins?". Sub-Cellular Biochemistry. 80: 271–291. doi:10.1007/978-94-017-8881-6_14. ISSN 0306-0225. PMID 24798017.
- ^ Sakurai, Nobuki; Kaneko, Jun; Kamio, Yoshiyuki; Tomita, Toshio (2004-07-13). "Cloning, expression, and pore-forming properties of mature and precursor forms of pleurotolysin, a sphingomyelin-specific two-component cytolysin from the edible mushroom Pleurotus ostreatus". Biochimica et Biophysica Acta. 1679 (1): 65–73. doi:10.1016/j.bbaexp.2004.05.002. ISSN 0006-3002. PMID 15245918.
- ^ Shibata, Taisei; Kudou, Makiko; Hoshi, Yoshinobu; Kudo, Ayako; Nanashima, Naoki; Miyairi, Kazuo (2010-12-01). "Isolation and characterization of a novel two-component hemolysin, erylysin A and B, from an edible mushroom, Pleurotus eryngii". Toxicon. 56 (8): 1436–1442. doi:10.1016/j.toxicon.2010.08.010. ISSN 1879-3150. PMID 20816689.
- ^ a b Bhat, Hema Balakrishna; Ishitsuka, Reiko; Inaba, Takehiko; Murate, Motohide; Abe, Mitsuhiro; Makino, Asami; Kohyama-Koganeya, Ayako; Nagao, Kohjiro; Kurahashi, Atsushi (2015-09-01). "Evaluation of aegerolysins as novel tools to detect and visualize ceramide phosphoethanolamine, a major sphingolipid in invertebrates". FASEB Journal. 29 (9): 3920–3934. doi:10.1096/fj.15-272112. ISSN 1530-6860. PMID 26060215.
{{cite journal}}: CS1 maint: unflagged free DOI (link) - ^ Berne, Sabina; Krizaj, Igor; Pohleven, Franc; Turk, Tom; Macek, Peter; Sepcić, Kristina (2002-04-15). "Pleurotus and Agrocybe hemolysins, new proteins hypothetically involved in fungal fruiting". Biochimica et Biophysica Acta. 1570 (3): 153–159. doi:10.1016/s0304-4165(02)00190-3. ISSN 0006-3002. PMID 12020804.
- ^ Berne, Sabina; Sepcić, Kristina; Anderluh, Gregor; Turk, Tom; Macek, Peter; Poklar Ulrih, Natasa (2005-08-23). "Effect of pH on the pore forming activity and conformational stability of ostreolysin, a lipid raft-binding protein from the edible mushroom Pleurotus ostreatus". Biochemistry. 44 (33): 11137–11147. doi:10.1021/bi051013y. ISSN 0006-2960. PMID 16101298.
- ^ Berne, Sabina; Lah, Ljerka; Sepcić, Kristina (2009-04-01). "Aegerolysins: structure, function, and putative biological role". Protein Science. 18 (4): 694–706. doi:10.1002/pro.85. ISSN 1469-896X. PMC 2762582. PMID 19309687.
As of 20:02, 16 February 2016 (UTC), this article is derived in whole or in part from Transporter Classification Database. The copyright holder has licensed the content in a manner that permits reuse under CC BY-SA 3.0 and GFDL. All relevant terms must be followed. The original text was at "1.C.97 The Pleurotolysin Pore-Forming (Pleurotolysin) Family"