Postzygotic mutation

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A post- zygotic mutation is a change in an organism's genome that is acquired during its lifespan, instead of being inherited from its parent(s) through fusion of two haploid gametes. Mutations that occur after the zygote has formed can be caused by a variety of sources that fall under two classes: spontaneous mutations and induced mutations. How detrimental a mutation is to an organism is dependent on what the mutation is, where it occurred in the genome and when it occurred.[1]

Causes

Main article: Mutagenesis

Post- zygotic changes to a genome can be caused by small mutations that affect a single base pair, or large mutations that affect entire chromosomes and are divided into two classes, spontaneous mutations and induced mutations.[2]

Spontaneous Mutations

Depurination mutation results in one normal strand and one shortened strand after replication.

Most spontaneous mutations are the result of naturally occurring lesions to DNA and errors during in DNA replication without direct exposure to an agent.[2] A few common spontaneous mutations are:

  • Depurination- The loss of a purine (A or G) base to form an apurinic site. An apurinic site, also known as an AP site, is the location in a genetic sequence that does not contain a purine base. During replication, the affected double stranded DNA will produce one doubled-stranded daughter containing the missing purine, resulting in an unchanged sequence. The other strand will produce a shorter strand, missing the purine and its complementary base.[2][3]
  • Deamination- The amine group on a base is changed to a keto group. This results in cytosine being changed to uracil and adenine being changed to hypoxanthine which can result in incorrect DNA replication and repair.[2][3]
  • Tautomerization- The hydrogen atom on a nucleotide base is repositioned causing altered hydrogen bonding pattern and incorrect base pairing during replication.[2] For example, the keto tautomer of thymine normally pairs with adenine, however the enol tautomer of thymine can bind with guanine. This results in an incorrect base pair match. Similarly there are amino and imino tautomers of cytosine and adenine that can cause incorrect base pairing with other nucleotides.[4]

Induced Mutations

Induced mutations are any lesions in DNA caused by an agent or mutagen. Mutagens often demonstrate mutational specificity, meaning they cause predictable changes in the DNA sequence.[5] A few common mutagens that induce mutations are:

  • Ultraviolet light (UV)- Causes pyrimidine (T or C) nucleotide bases on the same strand to covalent join together forming a pyrimidine dimer. Thymine-thymine dimers are the most common mutation caused by UV light. Since dimers cause a disruptive kink in DNA structure, polymerases often have trouble reading the region, slowing down DNA replication.[5]
  • Base Analogs- Chemical compounds that are sufficiently similar in structure and chemistry to the nitrogenous bases of DNA, such that they are able to be incorporated in the sequence. These analogs do not have the same pairing properties of normal bases, therefore they can pair incorrectly with nucleotides during replication. 5- bromouracil (5-BU) is a common analog to thymine, however the enol form of 5-BU is still able to bind with adenine. The ionized form, on the other hand, pairs with guanine.[5]
  • Intercalating agents- Chemical compounds that place themselves between stacked nitrogenous bases in DNA, causing a frameshift mutation. Some intercalating agents, like duanorubicin, are capable of blocking replication and transcription, making them incredibly toxic to proliferating cells.[5]
  • Reactive Oxygen Species (ROS)- Highly reactive oxygen- containing molecules that are capable causing DNA strand breaks and many damaging effects to cellular components.[5][6]
  • Alkylating agents- Compounds that attach an alkyl group to the four bases. When an alkyl group is added to guanine, it can lead to the incorrect pairing with thymine and disrupt the accuracy of replication.[5]

Consequences

A large determinant of the severity of consequences caused by post- zygotic mutations is when and where they occur. As a result consequences can range from being negligible to incredibly detrimental.[7]

Mosaicism

Mosaicism arises after the zygote has formed and a mutation occurs during development. The mutated cell line can be passed down to offspring if the germ- cells are affected.

When an individual has inherited an abnormality it is usually present in all of their cells. However some mutations like DNA code change, epigenetic alterations and chromosomal abnormalities, can occur later in development. This would result in one progeny cell line to be normal while the other cell line(s) to be abnormal. As a result the individual is considered to be a mosaic of normal and abnormal cells.[7]

Mosaicism is the occurrence of two or more cell lines with different genotypes within a single individual. It is different from chimerism which is the fusion of two zygotes, causing a new single zygote with two genotypes.[7]

Trisomy 21 Mosaicism

Trisomy 21 (Down Syndrome) is one of the most prevalent chromosomal abnormalities amongst live births. Of all trisomy 21 pregnancies, approximately 80% end in spontaneous abortions or still-births. 1-5% of people diagnosed with having Down Syndrome are actually in fact "high- grade" trisomy 21 mosaics. The rest of trisomy 21 mosaics are marked as "low- grade" mosaics, meaning the chromosomal mutation occurs in less than 3-5% of respective tissue. While high- grade trisomy 21 mosaics, demonstrate similar features to full Down Syndrome, low- grade mosaics have a tendency to show milder features, however the effects are quite variable depending on the distribution of the trisomic cells.[8]

Somatic mutations

Somatic mutations are the result of a change in the genetic structure after fertilization. This type of mutation also involves cells outside of the reproductive group and thus is not transmitted to future descendants.[9][10]

Germ- line mutations

Germ- line mutations are the result of a change in the genetic structure of germ cells. These mutations are able to be transmitted to the offspring and give rise to a constitutional mutation. Constitutional mutations is a mutation that when present in one cell, is also present in all other cells associated with the organism.[10]

References

  1. ^ Acuna-Hidalgo, Rocio; Bo, Tan; Kwint, Michael P.; van de Vorst, Maartje; Pinelli, Michele; Veltman, Joris A.; Hoischen, Alexander; Vissers, Lisenka E. L. M.; Gilissen, Christian (2015-07-02). "Post-zygotic Point Mutations Are an Underrecognized Source of De Novo Genomic Variation". The American Journal of Human Genetics. 97 (1): 67–74. doi:10.1016/j.ajhg.2015.05.008. PMC 4571017. PMID 26054435.
  2. ^ a b c d e Griffiths, Anthony JF; Miller, Jeffrey H.; Suzuki, David T.; Lewontin, Richard C.; Gelbart, William M. (2000-01-01). "Spontaneous mutations". {{cite journal}}: Cite journal requires |journal= (help)
  3. ^ a b Alberts, Bruce; Johnson, Alexander; Lewis, Julian; Raff, Martin; Roberts, Keith; Walter, Peter (2002-01-01). "DNA Repair". {{cite journal}}: Cite journal requires |journal= (help)
  4. ^ "Documents - All Documents". faculty.ksu.edu.sa. Retrieved 2015-12-02.
  5. ^ a b c d e f Griffiths, Anthony JF; Miller, Jeffrey H.; Suzuki, David T.; Lewontin, Richard C.; Gelbart, William M. (2000-01-01). "Induced mutations". {{cite journal}}: Cite journal requires |journal= (help)
  6. ^ Cooke, Marcus S.; Evans, Mark D.; Dizdaroglu, Miral; Lunec, Joseph (2003-07-01). "Oxidative DNA damage: mechanisms, mutation, and disease". The FASEB Journal. 17 (10): 1195–1214. doi:10.1096/fj.02-0752rev. ISSN 0892-6638. PMID 12832285.{{cite journal}}: CS1 maint: unflagged free DOI (link)
  7. ^ a b c Youssoufian, Hagop; Pyeritz, Reed E. (2002-10-01). "Mechanisms and consequences of somatic mosaicism in humans". Nature Reviews. Genetics. 3 (10): 748–758. doi:10.1038/nrg906. ISSN 1471-0056. PMID 12360233.
  8. ^ Hultén, Maj A.; Jonasson, Jon; Nordgren, Ann; Iwarsson, Erik (2010-09-01). "Germinal and Somatic Trisomy 21 Mosaicism: How Common is it, What are the Implications for Individual Carriers and How Does it Come About?". Current Genomics. 11 (6): 409–419. doi:10.2174/138920210793176056. ISSN 1389-2029. PMC 3018721. PMID 21358985.
  9. ^ "Somatic mutation - Glossary Entry". Genetics Home Reference. 2015-11-09. Retrieved 2015-11-14.
  10. ^ a b Griffiths, Anthony JF; Miller, Jeffrey H.; Suzuki, David T.; Lewontin, Richard C.; Gelbart, William M. (2000-01-01). "Somatic versus germinal mutation". {{cite journal}}: Cite journal requires |journal= (help)
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