Propidium iodide

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Propidium iodide
Propidium iodide.png
Identifiers
3D model (Jmol)
ChEBI
ChemSpider
ECHA InfoCard 100.042.786
Properties
C27H34I2N4
Molar mass 668.3946
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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Infobox references

Propidium iodide (or PI) is a fluorescent intercalating agent that can be used to stain cells. Propidium iodide is used as a DNA stain in flow cytometry to evaluate cell viability or DNA content in cell cycle analysis, [1] or in microscopy to visualise the nucleus and other DNA-containing organelles. Propidium Iodide cannot cross the membrane of live cells, making it useful to differentiate necrotic, apoptotic and healthy cells.[2][3] PI also binds to RNA, necessitating treatment with nucleases to distinguish between RNA and DNA staining.[4]


See also[edit]

References[edit]

  1. ^ "Propidium Iodide Solution - BioLegend". Retrieved 10 January 2015. 
  2. ^ Lecoeur H (2002). "Nuclear apoptosis detection by flow cytometry: influence of endogenous endonucleases". Exp. Cell Res. 277 (1): 1–14. doi:10.1006/excr.2002.5537. PMID 12061813. 
  3. ^ "Propidium Iodide". ThermoFisher. 
  4. ^ Suzuki T, Fujikura K, Higashiyama T, Takata K (1 January 1997). "DNA staining for fluorescence and laser confocal microscopy". J. Histochem. Cytochem. 45 (1): 49–53. doi:10.1177/002215549704500107. PMID 9010468.