PtK2 Cells are a cell line derived from male rat-kangaroo (Potorous tridactylis) epithelial kidney cells. This cell line is used for a variety of applications in biomedical research but is particularly popular as a model for mitosis.
The PtK2 cell line was established by Kristen Walen and Spencer Brown in 1962. Cells from the rat kangaroo were selected as the source of a cell line because this species has only a small number of chromosomes and these chromosomes are easily visualized under a microscope; mitosis becomes easy to observe, and the structural aspects of cell division can be closely examined.
PtK2 cells are relatively large, and when grown in a monolayer, stay flat throughout the cell cycle - unlike many cells that round up during mitosis. PtK2 cells are resistant to adenovirus 5, coxsackievirus B5, and poliovirus 2. They are susceptible to coxsackievirus A9, herpes simplex, vaccinia, and vesicular stomatitis (Ogden strain). PtK2 cells contain intermediate filaments composed of Keratin.
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- Walen, KH; Brown, SW (Apr 28, 1962). "Chromosomes in a marsupial (Potorous tridactylis) tissue culture". Nature. 194: 406. doi:10.1038/194406a0. PMID 14004541.
- Brescia, Jr, Peter J.; Banks, Peter (24 June 2014). "High Resolution Fluorescence Microscopy of PtK2 Cells Undergoing Mitosis in Microplates". BioTek. Retrieved 20 June 2018.
- "Molecular Expressions Microscopy Primer: Specialized Microscopy Techniques - Fluorescence Digital Image Gallery - Male Rat Kangaroo Kidney Epithelial Cells (PtK2)". Retrieved 17 December 2013.
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- "Olympus Microscopy Resource Center | Fluorescence Digital Image Gallery - Epithelial Cells". Retrieved 17 December 2013.