RNAI
| RNAI | |
|---|---|
 Predicted secondary structure and sequence conservation of RNAI | |
| Identifiers | |
| Symbol | RNAI |
| Rfam | RF00106 |
| Other data | |
| RNA type | Gene; antisense |
| Domain(s) | Bacteria |
| SO | SO:0000644 |
| PDB structures | PDBe |
RNAI is a non-coding RNA that is an antisense repressor of the replication of some E. coli plasmids, including ColE1. Plasmid replication is usually initiated by RNAII,[1] which acts as a primer by binding to its template DNA. The complementary RNAI binds RNAII prohibiting it from its initiation role. The rate of degradation of RNAI is therefore a major factor in control of plasmid replication. This rate of degradation is aided by the pcnB (plasmid copy number B) gene product,[2] which polyadenylates the 3' end of RNAI targeting it for degradation by PNPase.[3]
References
- ^ Masukata, Hisao; Tomizawa, J-I (1986). "Control of primer formation for ColE1 plasmid replication: conformational change of the primer transcript". Cell. 44: 125–136. doi:10.1016/0092-8674(86)90491-5. PMID 2416472.
- ^ He, L; Soderbom F; Wagner EG; Binnie U; Binns N; Masters M (1993). "PcnB is required for the rapid degradation of RNAI, the antisense RNA that controls the copy number of ColE1-related plasmids". Mol Microbiol. 9 (6): 1131–1142. doi:10.1111/j.1365-2958.1993.tb01243.x. PMID 7523833.
- ^ Xu, F; Lin-Chao S; Cohen SN (1993). "The Escherichia coli pcnB gene promotes adenylylation of antisense RNAI of ColE1-type plasmids in vivo and degradation of RNAI decay intermediates". Proc Natl Acad Sci USA. 90 (14): 6756–6760. doi:10.1073/pnas.90.14.6756. PMC 47011. PMID 7688127.
- Cohen, SN (1995). "Surprises at the 3' end of prokaryotic RNA". Cell. 80 (6): 829–832. doi:10.1016/0092-8674(95)90284-8. PMID 7535193.
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