TxpA-RatA toxin-antitoxin system

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RNA anti-toxin A
RatA SScons.png
Conserved secondary structure of RatA antitoxin sRNA.
Symbol RatA
Rfam RF01776
Other data
RNA type Antisense
Domain(s) Firmicutes
SO {{{SO}}}
PDB structures PDBe

The TxpA/RatA toxin-antitoxin system was first identified in Bacillus subtilis.[1] It consists of a non-coding 222nt sRNA called RatA (RNA anti-toxin A) and a protein toxin named TxpA (Toxic protein A).[2]

RatA was discovered in intergenic regions of the B. subtilis genome, in a 728-nucleotide region between genes yqdB (later renamed TxpA) and yqbM. Initially, Affymetrix microarrays were used to detect transcription in this region,[3] Northern blot experiments and mutation analysis were then employed to characterise the RNA transcript.[1]

ratA and txpA are transcribed convergently, and have an overlap of 75 base pairs at their 3' ends, providing an area of complimentarity. The RatA transcript binds with the TxpA mRNA across the complimentary region[1] and the dsRNA is then degraded by an uncharacterised RNase E equivalent, preventing translation of the toxic TxpA protein.[2]

Genes ratA and txpA are found within a 48kb phage-like element called skin. This element interrupts a gene for the sigma factor σK and is excised during sporulation. The toxin-antitoxin system contained within skin forces the inheritance of this element, which is acting as a selfish gene.[1]

The mechanism by which TxpA induces cell lysis and death is unknown. TxpA is not similar enough to other proteins of known function to infer a related function, however it does have a suspected transmembrane region in its N-terminal, so it is possible that TxpA damages the integrity of the cell membrane, or blocks cell wall synthesis.[1]

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  1. ^ a b c d e Silvaggi JM, Perkins JB, Losick R (October 2005). "Small untranslated RNA antitoxin in Bacillus subtilis". J. Bacteriol. 187 (19): 6641–50. doi:10.1128/JB.187.19.6641-6650.2005. PMC 1251590Freely accessible. PMID 16166525. Retrieved 2010-09-16. 
  2. ^ a b Fozo EM, Makarova KS, Shabalina SA, Yutin N, Koonin EV, Storz G (June 2010). "Abundance of type I toxin-antitoxin systems in bacteria: searches for new candidates and discovery of novel families". Nucleic Acids Res. 38 (11): 3743–59. doi:10.1093/nar/gkq054. PMC 2887945Freely accessible. PMID 20156992. Retrieved 2010-09-16. 
  3. ^ Lee JM, Zhang S, Saha S, Santa Anna S, Jiang C, Perkins J (December 2001). "RNA expression analysis using an antisense Bacillus subtilis genome array". J. Bacteriol. 183 (24): 7371–80. doi:10.1128/JB.183.24.7371-7380.2001. PMC 95586Freely accessible. PMID 11717296. Retrieved 2010-09-16. 

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