Tosyl phenylalanyl chloromethyl ketone
3D model (JSmol)
|Molar mass||351.848 g/mol|
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
|what is ?)(|
TPCK is observed covalently bound in the active site of Caspase 3 in the crystal structure of the complex solved in 2010. The chloromethyl group reacts with the active site cysteine to form a covalent bond with the loss of the chlorine.
TPCK is chosen for the chemical labelling of active histidine in enzyme analysis. The phenylalanine moiety is bound to the enzyme because of specificity for aromatic amino acid residues at the active site (as in chymotrypsin, in which it binds to the Histidine-57 residue in the active site).
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