U-87 MG
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In cell biology, U87 is a human primary glioblastoma cell line formally known as U-87 MG (abbreviation for Uppsala 87 Malignant Glioma). It has epithelial morphology, and was obtained from a stage four 44-year-old cancer patient.[1] In 2016, the commonly used version of U87MG was found to be non-identical to its patient of origin.[2]
U-87 MG can be obtained from the American Type Culture Collection (ATCC) where it is known by the accession number HTB-14.
The entire sequence of the genome of U-87 MG has been published in PLoS Genetics, 2010 January; 6(1): e1000832.
Growth conditions
U87 growth media is generally made with Eagle's minimum essential medium + 10% FBS + 100 U/ml penicillin + 100 ug/ml streptomycin.[3] It is propagated at 37 degrees Celsius in a 5% carbon dioxide atmosphere.
References
- ^ "U87MG decoded: the genomic sequence of a cytogenetically aberrant human cancer cell line". PLoS Genet. 6 (1): e1000832. January 2010. doi:10.1371/journal.pgen.1000832. PMC 2813426. PMID 20126413.
{{cite journal}}: CS1 maint: unflagged free DOI (link) - ^ "Origin of the U87MG glioma cell line: Good news and bad news". Sci Transl Med. Aug 2016. PMID 27582061.
- ^ "HTB-14". ATCC.
External links
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