User:HermiaStudy/Pseudomonas
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Article Draft
[edit]NOTE: Had to move table and rewrite on species Pseudomonas aeruginosa for specific work instead of just the genus.
[edit]Genomics(Original Paragraph)
[edit]In 2000, the complete genome sequence of a Pseudomonas species was determined; more recently, the sequence of other strains has been determined, including P. aeruginosa strains PAO1 (2000), P. putida KT2440 (2002), P. protegens Pf-5 (2005), P. syringae pathovar tomato DC3000 (2003), P. syringae pathovar syringae B728a (2005), P. syringae pathovar phaseolica 1448A (2005), P. fluorescens Pf0-1, and P. entomophila L48.
By 2016, more than 400 strains of Pseudomonas had been sequenced. Sequencing the genomes of hundreds of strains revealed highly divergent species within the genus. In fact, many genomes of Pseudomonas share only 50-60% of their genes, e.g. P. aeruginosa and P. putida share only 2971 proteins out of 5350 (or ~55%).
By 2020, more than 500 complete Pseudomonas genomes were available in Genebank. A phylogenomic analysis utilized 494 complete proteomes and identified 297 core orthologues, shared by all strains. This set of core orthologues at the genus level was enriched for proteins involved in metabolism, translation, and transcription and was utilized for generating a phylogenomic tree of the entire genus, to delineate the relationships among the Pseudomonas major evolutionary groups. In addition, group-specific core proteins were identified for most evolutionary groups, meaning that they were present in all members of the specific group, but absent in other Pseudomonads. For example, several P. aeruginosa-specific core proteins were identified that are known to play an important role in this species' pathogenicity, such as CntL, CntM, PlcB, Acp1, MucE, SrfA, Tse1, Tsi2, Tse3, and EsrC.
Things to change:
- Some sentence structures.
- Find more research on genomes of Pseudomonas
- Make it more interesting? Paragraph is a bit of a drag
- Characteristics of Pseudomonas.
Morphological, physiological, and biochemical characteristics of Pseudomonas are shown in the Table below.
| Test type | Test | Characteristics |
| Colony characters | Size | |
| Type | ||
| Color | ||
| Shape | ||
| Morphological characters | Shape | Rod[1] |
| Physiological characters | Motility | +[1] |
| Growth at 6.5% NaCl | ||
| Biochemical characters | Gram staining | -[1] |
| Oxidase | +[1] | |
| Catalase | +[1] | |
| Oxidative-Fermentative | ||
| Motility | +[1] | |
| Methyl Red | -[1] | |
| Voges-Proskauer | -[1] | |
| Indole | -[1] | |
| H2S Production | ||
| Urease | ||
| Nitrate reductase | ||
| β-Galactosidase | ||
| Hydrolysis of | Gelatin | |
| Casein | ||
| Utilization of | Glycerol | |
| Galactose | ||
| D-Glucose | ||
| D-Fructose | ||
| D-Mannose | ||
| Mannitol | ||
Note: + = Positive, – =Negative