Wikipedia:United States Education Program/Courses/JHU MolBio Ogg 2013/Group 82G

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Group 82G[edit]

This is the Wikipedia page for 410.602 Molecular Biology, Spring, 2013, group 82G. This group will be working on the article Frameshift mutation.

Use the talk page here to collaborate as a group, when learning to use and navigate Wikipedia, assessing articles, or for any other topic.

Use this page (not the talk page) for article assessments; rationale for selecting an article; etc.

Please create a new section here for each of those assignments.

Initial Article Assessment Sharkeyr[edit]

Frameshift Mutation[edit]

This article does not contain much information, though the information it does contain is useful. It has very few references to back up most of what is said, and not all of the references are cited correctly. According to the Wikiproject for Molecular and Cellular biology it is of high importance that it is updated. There is only 1 person with input on the talk page, though the comments are very useful for things to not only add, but things within the article that are incorrect. What is there is written in a decent matter, but could definitely use some editing. There is a lot more information it could include, it is pretty narrowly focused. It is very neutral in its tone and the information that is there is stable.

references[edit]

  1. ^ Ripley, L S (1990). "Frameshift Mutation: Determinants of Specificity". Annual Review of Genetics. 24 (1): 189–211. doi:10.1146/annurev.ge.24.120190.001201. PMID 2088167.
  2. ^ Strauss, BS (November 1999). "Frameshift mutation, microsatellites and mismatch repair". Mutation Research. 437 (3): 195–203. doi:10.1016/s1383-5742(99)00066-6. PMID 10592327.
  3. ^ Chung, H.; Lopez, C. G.; Holmstrom, J.; Young, D. J.; Lai, J. F.; Ream-Robinson, D.; Carethers, J. M. (2010). "Both microsatellite length and sequence context determine frameshift mutation rates in defective DNA mismatch repair". Human Molecular Genetics. 19 (13): 2638–2647. doi:10.1093/hmg/ddq151. PMC 2912546. PMID 20418486.

Coding Region[edit]

This article is only 2 paragraphs long, so there is not much information available. There are also only 3 references. It is of high importance according to the Molecular and Cellular Biology Wikiproject. There is only 1 comment on the talk page, dating back to 2011. It is not very well written and could use editing as well as content. The focus is extremely narrow, as it is only a few sentences. Though it is neutral in its tone, and the information given is stable.

references[edit]

  1. ^ Park, Chungoo; Makova, Kateryna D (2009). "Coding region structural heterogeneity and turnover of transcription start sites contribute to divergence in expression between duplicate genes". Genome Biology. 10 (1): R10. doi:10.1186/gb-2009-10-1-r10. PMID 19175934.
  2. ^ Fang, Zhuo; Rajewsky, Nikolaus; Deb, Sumitra (2011). "The Impact of miRNA Target Sites in Coding Sequences and in 3′UTRs". PLOS ONE. 6 (3): e18067. Bibcode:2011PLoSO...618067F. doi:10.1371/journal.pone.0018067. PMC 3062573. PMID 21445367.
  3. ^ Loh, E.; Memarpour, F.; Vaitkevicius, K.; Kallipolitis, B. H.; Johansson, J.; Sonden, B. (2012). "An unstructured 5'-coding region of the prfA mRNA is required for efficient translation". Nucleic Acids Research. 40 (4): 1818–1827. doi:10.1093/nar/gkr850. PMC 3287183. PMID 22053088.

Initial Article Assessment from DougCovert[edit]

N-terminus[edit]

This article has no footnotes or references and thus no inline citations which may indicate inaccuracies may be present in the information provided. In the textbook by Voet and Voet baseline information is provided on the N-terminus[1]. Only two people have made comments in the talk section indicating no substantial ways for improvement. The comments were last done in 2007. It flows well and has a neutral viewpoint, but is in need of additional information. Under its function section its give a small amount of information but suggests going to other links. A paper by Meninnel suggests the importance of the N-terminal as a signal in protein degradation.[2] There is only one image that is not tagged with copyright status.

References[edit]

  1. ^ Voet, Donald; Voet, Judith G. (2004). Biochemistry (3rd ed.). Hoboken, N.J.: John Wiley & Sons. pp. 71, 1318–1320. ISBN 0-471-19350-X.
  2. ^ Meinnel, T; Serero, A; Giglione, C (July 2006). "Impact of the N-terminal amino acid on targeted protein degradation". Biological Chemistry. 387 (7): 839–51. doi:10.1515/BC.2006.107. PMID 16913833.

Ribosomal RNA[edit]

When looking into the talk page of this article comments were originally made in 2007 and the last in 2010. They indicated improvements in providing further information regarding comparisons with tRNA and mRMA and also that most of the information in this article is about the ribosome. The textbook by Watson, et al. has a chapter on translation that would help in providing these comparisons[1]. It does provide a neutral point of view and is written satisfactory and flow rather disjointed. There are two images not tagged with copyright status. It does not have citations in main informational sections and lacks some areas required by the WikiProject MCB. Further improvements could be made by adding additional information on how ribosomal RNA mature and degrade[2]

References[edit]

  1. ^ al.], James D. Watson ... [et (2007). Molecular biology of the gene (6th. ed.). San Francisco, Calif.: Benjamin Cummings. ISBN 978-0-8053-9592-1.
  2. ^ Deutscher, MP (2009). "Maturation and degradation of ribosomal RNA in bacteria". Progress in Molecular Biology and Translational Science. 85: 369–91. doi:10.1016/S0079-6603(08)00809-X. PMID 19215777.

Article Selection Rationale[edit]

Group 82G selected frameshift mutation because this topic has limited information and few references. From these two major observations, and the fact that the Wikiproject for Molecular and Cellular biology rate this as High for improvement, work on this article is required. Even though the article was satisfactory written, it presents a narrow focus on the subject of frameshift mutation. There are more key points, or concepts that will help develop this article from a Stub status. It’s an intriguing topic, as a simple/small change in a nucleotide can cause such large/complex outcomes. To begin, and most importantly, there are a number of different diseases with no known cures that are the result of making the wrong protein from reading the codon. There are projects for several disease types looking specifically at frameshift mutations that was not mentioned in the original article. Also, more research data into what causes a frameshift mutation to occur are available for learning and understanding that can be effectively expanded upon in more detail. We can look further into gRNA which is described in the textbook as a way frameshift mutations occur. The article did indicate a couple of areas of research into the exact process on how the shift (insertion or deletion) occurs, and thus are key points to upgrade. The original authors did provide direction for further reading and external links, and these will both be great starting points to enlarge this article’s reference base. Much of the information on the site comes from references dating back to 1996. With the expansion of genomics, there is currently much more research and information available. Finally, there is no referencing in the article other type of point mutation and how it differs from a frameshift mutation to give background on the importance of the mutation. Some additional links that may be of use are listed below: http://www.sanger.ac.uk/genetics/CGP/Census/frameshift_mutation.shtml

http://www.medscape.com/viewarticle/584255

http://vir.sgmjournals.org/content/85/8/2389.full.pdf

Unit 9 Progress Report[edit]

1. Structure: • Set up headings (table of contents) for the specific sections following our outline and improvement paragraph. Also made minor rearrangements of original information already in the article. Started information input into sections using correct citation methods for references. • Uploaded images from wiki commons to correspond with the particular topic outlined in the table of contents. Examples are the central dogma, translation, codon, and specific diseases images.

2. Background • Topics included central dogma, transcription & translation and the importance of the codon. The purpose of this was to provide a generic background for the non-science person and show importance of transfer of genetic material. • Also, included examples on certain repair systems that would help to ensure correct transfer of genetic information.

3. Mechanism • Provided Information on the types of mutations and how each of them function differently. • Discussed possibility of genetic vs. environmental causes

4. Causes • In this section inputted examples of specific types of research on finding the causes of frameshift mutation. Examples ranged from sequencing methods to sampling bodily fluids.

5. Diseases • The types of disease caused from frameshift mutation were described. Added onto Tay-Sachs, included Crohn’s disease and others.. Also found other types not so reported about in the media like Hypertrophic Cardiomyopathy(sudden death). • Cures for frameshift mutation was another topic in this section. Specific examples for gene therapy were discussed.

Final Progress Report[edit]

1. General Structure Changes - added a small introduction under each heading - include ‘main article’ links where possible - linked to other articles within the individual topics where merited - add more references and images where required


2. Lead - Reformatted the information in this section by combining sentences to make two concise paragraphs detaining the article

3. Background - Streamlined the information about translation to make it more readable and to the point.

4. Mechanism - Deleted information from and moved the mutation part further down in the topic where it made better sense. - Increased information about finding, and the frequency of frameshift mutations.

5. Diseases - Added more (cancers, HIV)and increased the data regarding the types of diseases from this mutation - Created a section about potential cures

6. Reviewers - Thank you to the peers that reviewed this article. Also, thanks to our OA, and other Wikipedians not involved with this course that added comments. With your edit suggestions the article became better.

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