ARFGEF1

ARFGEF1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 3LTL, 5EE5
Identifiers
Aliases	ARFGEF1, ARFGEP1, BIG1, P200, ADP ribosylation factor guanine nucleotide exchange factor 1
External IDs	OMIM: 604141; MGI: 2442988; HomoloGene: 4687; GeneCards: ARFGEF1; OMA:ARFGEF1 - orthologs
Gene location (Human) Chr. Chromosome 8 (human) Band 8q13.2 Start 67,173,511 bp End 67,343,781 bp
Gene location (Mouse) Chr. Chromosome 1 (mouse) Band 1|1 A2 Start 10,207,796 bp End 10,302,895 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in gonad secondary oocyte epithelium of nasopharynx sural nerve bronchial epithelial cell testicle mucosa of sigmoid colon jejunal mucosa bone marrow tibia Top expressed in gastrula crypt of lieberkuhn of small intestine submandibular gland transitional epithelium of urinary bladder cumulus cell zygote knee joint triceps brachii muscle cardiac muscle tissue of left ventricle plantaris muscle More reference expression data BioGPS More reference expression data
Gene ontology Molecular function GTPase activator activity guanyl-nucleotide exchange factor activity myosin binding protein binding protein kinase A regulatory subunit binding Cellular component cytosol Golgi apparatus membrane nuclear matrix Golgi membrane nucleoplasm trans-Golgi network nucleolus small nuclear ribonucleoprotein complex perinuclear region of cytoplasm nucleus cytoplasm Biological process endomembrane system organization positive regulation of protein kinase B signaling negative regulation of actin filament polymerization Golgi organization positive regulation of wound healing regulation of ARF protein signal transduction negative regulation of GTPase activity protein transport neuron projection development regulation of establishment of cell polarity exocytosis positive regulation of GTPase activity vesicle-mediated transport Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 10565 211673 Ensembl ENSG00000066777 ENSMUSG00000067851 UniProt Q9Y6D6 G3X9K3 RefSeq (mRNA) NM_006421 NM_001102430 RefSeq (protein) NP_006412 NP_001095900 Location (UCSC) Chr 8: 67.17 – 67.34 Mb Chr 1: 10.21 – 10.3 Mb PubMed search
Wikidata
View/Edit Human View/Edit Mouse

Brefeldin A-inhibited guanine nucleotide-exchange protein 1 is a protein that in humans is encoded by the ARFGEF1 gene.

Function

ADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. The protein encoded by this gene is involved in the activation of ARFs by accelerating replacement of bound GDP with GTP. It contains a Sec7 domain, which may be responsible for the guanine-nucleotide exchange activity and also the brefeldin A inhibition.

Interactions

ARFGEF1 has been shown to interact with FKBP2, ARFGEF2 and PRKAR1A.

External links

• Human ARFGEF1 genome location and ARFGEF1 gene details page in the UCSC Genome Browser.

Further reading

• Bonaldo MF, Lennon G, Soares MB (1997). "Normalization and subtraction: two approaches to facilitate gene discovery". Genome Research. 6 (9): 791–806. doi:10.1101/gr.6.9.791. PMID 8889548.
• Mansour SJ, Skaug J, Zhao XH, Giordano J, Scherer SW, Melançon P (Jul 1999). "p200 ARF-GEP1: a Golgi-localized guanine nucleotide exchange protein whose Sec7 domain is targeted by the drug brefeldin A". Proceedings of the National Academy of Sciences of the United States of America. 96 (14): 7968–73. doi:10.1073/pnas.96.14.7968. PMC 22171. PMID 10393931.
• Yamaji R, Adamik R, Takeda K, Togawa A, Pacheco-Rodriguez G, Ferrans VJ, Moss J, Vaughan M (Mar 2000). "Identification and localization of two brefeldin A-inhibited guanine nucleotide-exchange proteins for ADP-ribosylation factors in a macromolecular complex". Proceedings of the National Academy of Sciences of the United States of America. 97 (6): 2567–72. doi:10.1073/pnas.97.6.2567. PMC 15969. PMID 10716990.
• Kamei Y, Takeda Y, Teramoto K, Tsutsumi O, Taketani Y, Watanabe K (Oct 2000). "Human NB-2 of the contactin subgroup molecules: chromosomal localization of the gene (CNTN5) and distinct expression pattern from other subgroup members". Genomics. 69 (1): 113–9. doi:10.1006/geno.2000.6310. PMID 11013081.
• Ota T, Suzuki Y, Nishikawa T, Otsuki T, Sugiyama T, Irie R, et al. (Jan 2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nature Genetics. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
• Padilla PI, Pacheco-Rodriguez G, Moss J, Vaughan M (Mar 2004). "Nuclear localization and molecular partners of BIG1, a brefeldin A-inhibited guanine nucleotide-exchange protein for ADP-ribosylation factors". Proceedings of the National Academy of Sciences of the United States of America. 101 (9): 2752–7. doi:10.1073/pnas.0307345101. PMC 365693. PMID 14973189.
• Saeki N, Tokuo H, Ikebe M (Mar 2005). "BIG1 is a binding partner of myosin IXb and regulates its Rho-GTPase activating protein activity". The Journal of Biological Chemistry. 280 (11): 10128–34. doi:10.1074/jbc.M413415200. PMID 15644318.
• Citterio C, Jones HD, Pacheco-Rodriguez G, Islam A, Moss J, Vaughan M (Feb 2006). "Effect of protein kinase A on accumulation of brefeldin A-inhibited guanine nucleotide-exchange protein 1 (BIG1) in HepG2 cell nuclei". Proceedings of the National Academy of Sciences of the United States of America. 103 (8): 2683–8. doi:10.1073/pnas.0510571103. PMC 1413798. PMID 16467138.
• Belov GA, Altan-Bonnet N, Kovtunovych G, Jackson CL, Lippincott-Schwartz J, Ehrenfeld E (Jan 2007). "Hijacking components of the cellular secretory pathway for replication of poliovirus RNA". Journal of Virology. 81 (2): 558–67. doi:10.1128/JVI.01820-06. PMC 1797456. PMID 17079330.
• Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M (Nov 2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573.
• Shen X, Hong MS, Moss J, Vaughan M (Jan 2007). "BIG1, a brefeldin A-inhibited guanine nucleotide-exchange protein, is required for correct glycosylation and function of integrin beta1". Proceedings of the National Academy of Sciences of the United States of America. 104 (4): 1230–5. doi:10.1073/pnas.0610535104. PMC 1783117. PMID 17227842.
• Kuroda F, Moss J, Vaughan M (Feb 2007). "Regulation of brefeldin A-inhibited guanine nucleotide-exchange protein 1 (BIG1) and BIG2 activity via PKA and protein phosphatase 1gamma". Proceedings of the National Academy of Sciences of the United States of America. 104 (9): 3201–6. doi:10.1073/pnas.0611696104. PMC 1805514. PMID 17360629.