Biotinylated retinoids are derivatives of retinol (vitamin A) carrying a biotin group for use in the isolation and purification of Retinol Binding Proteins involved in the visual cycle. The first biotinylated retinoid was synthesized in 2002  and was used in the isolation and characterization of RPE65.
Notes and references
- Nesnas, N.; Rando, R. R.; Nakanishi, K. (2002). "Synthesis of biotinylated retinoids for cross-linking and isolation of retinol binding proteins" (PDF). Tetrahedron 58 (32): 6577. doi:10.1016/S0040-4020(02)00667-1.
- Jahng, W. J.; David, C.; Nesnas, N.; Nakanishi, K.; Rando, R. R. (2003). "A Cleavable Affinity Biotinylating Agent Reveals a Retinoid Binding Role for RPE65†". Biochemistry 42 (20): 6159–6168. doi:10.1021/bi034002i. PMID 12755618.