10 examples were built by Dewoitine for assembly by the Swiss factory EKW in Switzerland. These were followed by an order for two more aircraft equipped with a slightly higher-powered version of the Wright 9Q engine that powered the initial batch, and one of the original D.26s was similarly re-engined. The original D.26s were used principally for training in gunnery and formation flying, while the more powerful aircraft were used for air-to-air combat training. To this end, they were equipped with gun cameras.
The D.26 enjoyed a long service life, not being withdrawn until 1948. At this time, they were transferred to the Aero-Club der Schweiz where they were used as glider tugs. The last example was not retired from aeroclub use until 1970, whereupon it was preserved at the military aviation museum at Dübendorf.