Epitope binning
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Epitope binning is a competitive immunoassay used to characterize and then sort a library of monoclonal antibodies against a target protein.[1] Antibodies against a similar target are tested against all other antibodies in the library in a pairwise fashion to see if antibodies block one another's binding to the epitope of an antigen.[2] After each antibody has a profile created against all of the other antibodies in the library, a competitive blocking profile is created for each antibody relative to the others in the library. Closely related binning profiles indicate that the antibodies have the same or a closely related epitope and are "binned" together.[2] Epitope binning is referenced in the literature under different names such as epitope mapping and epitope characterization.[2] Regardless of the naming, epitope binning is prevalent in the pharmaceutical industry.[2] Epitope Binning is used in the discovery and development of new therapeutics, vaccines, and diagnostics.[3]
See also
References
- ^ Abdiche, Y. N.; D. S. Malashock; A. Pinkerton; J. Pons (March 2009). "Exploring blocking assays using Octet, ProteOn, and Biacore biosensors". Analytical Biochemistry. 386 (2): 172–180. doi:10.1016/j.ab.2008.11.038. PMID 19111520.
- ^ a b c d Brooks, B.D. (2014). "The Importance of Epitope Binning in Drug Discovery". Current Drug Discovery Technologies. 11 (2): 109–12. doi:10.2174/1570163810666131124233827. PMC 4033553. PMID 24266537.
- ^ Estep, P.; Reid F; Nauman C; Liu Y; Sun T; Sun J; Xu Y. (2013). "High throughput solution-based measurement of antibody-antigen affinity and epitope binning". mAbs. 5 (2): 270–278. doi:10.4161/mabs.23049. PMC 3893237. PMID 23575269.
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