From Wikipedia, the free encyclopedia
Integrator complex subunit 12 (Int12) also known as PHD finger protein 22 (PHF22) is a protein that in humans is encoded by the INTS12 gene .[ 4]
INTS12 is a subunit of the Integrator complex , which associates with the C-terminal domain of RNA polymerase II large subunit (POLR2A ) and mediates 3-prime end processing of small nuclear RNAs U1 (RNU1 ) and U2 (RNU2 )[ 4] [ 5]
Model organisms
Model organisms have been used in the study of INTS12 function. A conditional knockout mouse line, called Ints12tm1a(EUCOMM)Wtsi [ 10] [ 11] International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists.[ 12] [ 13] [ 14]
Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.[ 8] [ 15] mutant mice and two significant abnormalities were observed.[ 8] homozygous mutant embryos were identified during gestation, and therefore none survived until weaning . The remaining tests were carried out on heterozygous mutant adult mice; no additional significant abnormalities were observed in these animals.[ 8]
References
^ a b c GRCm38: Ensembl release 89: ENSMUSG00000028016 – Ensembl , May 2017^ "Human PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ "Mouse PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ a b "Entrez Gene: integrator complex subunit 12" . Retrieved 2011-08-30 .^ Baillat D, Hakimi MA, et al. (2005). "Integrator, a multiprotein mediator of small nuclear RNA processing, associates with the C-terminal repeat of RNA polymerase II" . Cell . 123 (2): 265–76. doi :10.1016/j.cell.2005.08.019 PMID 16239144 . S2CID 18069461 . ^ "Salmonella infection data for Ints12" . Wellcome Trust Sanger Institute.^ "Citrobacter infection data for Ints12" . Wellcome Trust Sanger Institute.^ a b c d Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica . 88 : 925–7. doi :10.1111/j.1755-3768.2010.4142.x . S2CID 85911512 . ^ Mouse Resources Portal , Wellcome Trust Sanger Institute.^ "International Knockout Mouse Consortium" .^ "Mouse Genome Informatics" .^ Skarnes, W. C.; Rosen, B.; West, A. P.; Koutsourakis, M.; Bushell, W.; Iyer, V.; Mujica, A. O.; Thomas, M.; Harrow, J.; Cox, T.; Jackson, D.; Severin, J.; Biggs, P.; Fu, J.; Nefedov, M.; De Jong, P. J.; Stewart, A. F.; Bradley, A. (2011). "A conditional knockout resource for the genome-wide study of mouse gene function" . Nature . 474 (7351): 337–342. doi :10.1038/nature10163 . PMC 3572410 PMID 21677750 . ^ Dolgin E (2011). "Mouse library set to be knockout" . Nature . 474 (7351): 262–3. doi :10.1038/474262a PMID 21677718 . ^ Collins FS, Rossant J, Wurst W (2007). "A Mouse for All Reasons" . Cell . 128 (1): 9–13. doi :10.1016/j.cell.2006.12.018 PMID 17218247 . S2CID 18872015 . ^ van der Weyden L, White JK, Adams DJ, Logan DW (2011). "The mouse genetics toolkit: revealing function and mechanism" . Genome Biol . 12 (6): 224. doi :10.1186/gb-2011-12-6-224 . PMC 3218837 PMID 21722353 . {{cite journal }}: CS1 maint: unflagged free DOI (link )
Further reading
Kalsi G, Kuo PH, Aliev F, et al. (2010). "A systematic gene-based screen of chr4q22-q32 identifies association of a novel susceptibility gene, DKK2, with the quantitative trait of alcohol dependence symptom counts" . Hum. Mol. Genet . 19 (12): 2497–506. doi :10.1093/hmg/ddq112 . PMC 2876884 PMID 20332099 . Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)" . Genome Res . 14 (10B): 2121–7. doi :10.1101/gr.2596504 . PMC 528928 PMID 15489334 .
