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OSI may refer to:
- Osijek Airport, with IATA code "OSI", an airport in Croatia
- Ősi, Veszprém county, Hungary; a village
- Ido-Osi, Ekiti State, Nigeria; which contains a village called "Osi"
- OSI Group, an American holding company of meat processors that service the retail and food service industries
- OSI Pharmaceuticals, an American pharmaceutical company
- OSI Restaurant Partners, the restaurant and entertainment group that includes Outback Steakhouse
- OSI Systems, a company that manufactures security scanners and medical equipment based in California
- Objective Systems Integrators, a company that provides monitoring software for communication networks
- Open Systems International, an automation software vendor for the electric, petroleum, transportation and water industries
- Officine Stampaggi Industriali, defunct Italian coachbuilder
- Office of Scientific Intelligence, former name of a department of the Central Intelligence Agency now called the Directorate of Science and Technology
- Office of Strategic Influence, a short-lived former American government agency created in 2001, part of the Department of Defense and focused on psychological warfare
- Office of Special Investigations (disambiguation), a name shared by several government agencies
- Office of Scientific Integrity, now United States Office of Research Integrity
- Office of Special Investigation (Republic of Korea), a secret intelligence unit of Republic of Korea Air Force
- Olomouc Summer Institute, an educational program for high-school students
- Open Space Institute, an organization that seeks to preserve scenic, natural and historic landscapes
- Open Society Institute, a private charitable foundation established by George Soros to promote open societies around the world
- Ordnance Survey Ireland (Suirbhéireacht Ordanáis Éireann)
- Otto-Suhr-Institut, the political-science institute of the Free University of Berlin
- OSI, acronym for Official Sector Involvement in sovereign debt restructuring deals, as opposed to PSI (Private Sector Involvement).
- Options Symbology Initiative, a plan to change the way that exchange-traded options are named
- Open Systems Interconnection, (ISO 7498) a joint ISO and ITU-T standard for computer networks and communication protocols
- Open Source Initiative, an organization dedicated to promoting open source software
- Ohio Scientific Inc., a microcomputer manufacturer from the late 1970s to the early 1980s
- Open Switching Interval, a type of disconnect supervision
- On-Site Inspection, an advanced measure technique combining several methods - especially used in order to detect nuclear tests
- Officine Stampaggi Industriali, Italian coachbuilder
- OSI (band), an American progressive rock supergroup named after the former Office of Strategic Influence government agency
- Office of Strategic Influence (album), the 2003 debut album by the band OSI
- Origin Systems, a former United States computer game developer
- "Office of Scientific Intelligence", a fictional United States government agency in several television programs and movies
- "Office of Secret Intelligence", a fictional United States government secret agency in the animated series The Venture Bros.
- Osis (disambiguation)
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