para-Nitrophenylphosphate

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para-Nitrophenylphosphate
Skeletal formula
Space-filling model
Names
IUPAC name
(4-nitrophenyl) dihydrogen phosphate
Other names
pNPP
Identifiers
33013-2 YesY
ChEBI CHEBI:17440 N
ChEMBL ChEMBL24231 N
Jmol-3D images Image
PubChem 378
Properties
C6H6NO6P
Molar mass 219.088701
Except where noted otherwise, data is given for materials in their standard state (at 25 °C (77 °F), 100 kPa)
 N verify (what isYesY/N?)
Infobox references

para-Nitrophenylphosphate (pNPP) is a chromogenic substrate for acid and alkaline phosphatase in ELISA and conventional spectrophotometric assays. These enzymes catalyse the hydrolysis of pNPP to yellow para-nitrophenol. This product absorbs strongly at 405 nm and can be measured with a visible light spectrophotometer.

PNPP+H_2O \; \overrightarrow{_{AP}} \; PNP + P_i

External links[edit]