para-Nitrophenylphosphate

para-Nitrophenylphosphate
Identifiers
CAS number 33013-2
PubChem 378
ChEBI CHEBI:17440
ChEMBL CHEMBL24231
Jmol-3D images Image 1
Properties
Molecular formula C6H6NO6P
Molar mass 219.088701
Except where noted otherwise, data are given for materials in their standard state (at 25 °C (77 °F), 100 kPa)
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Infobox references

para-Nitrophenylphosphate (pNPP) is a chromogenic substrate for acid and alkaline phosphatase in ELISA assays. Under their influence the decay to yellow para-nitrophenol is catalysed. This product can be measured with a 405 nm spectrophotometer.

$PNPP+H_2O \; \overrightarrow{_{AP}} \; PNP + P_i$