(4-nitrophenyl) dihydrogen phosphate
Except where noted otherwise, data is given for materials in their standard state (at 25 °C (77 °F), 100 kPa)
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para-Nitrophenylphosphate (pNPP) is a chromogenic substrate for acid and alkaline phosphatase in ELISA and conventional spectrophotometric assays. These enzymes catalyse the hydrolysis of pNPP to yellow para-nitrophenol. This product absorbs strongly at 405 nm and can be measured with a visible light spectrophotometer.
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