Talk:Deoxycytidine kinase

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Wiki Education Foundation-supported course assignment[edit]

This article is or was the subject of a Wiki Education Foundation-supported course assignment. Further details are available on the course page. Student editor(s): Cmcheng28. Peer reviewers: Ricech.

Above undated message substituted from Template:Dashboard.wikiedu.org assignment by PrimeBOT (talk) 19:19, 16 January 2022 (UTC)[reply]

Outline for edits[edit]

For the introduction paragraph, I would like to add these two sentences and add 5 additional references to the article:

"Deoxycytidine kinase (dCK) predominantly phosphorylates deoxycytidine (dC) and converts dC into deoxycytidine monophosphate. dCK catalyzes one of the initial steps in the nucleotide salvage pathway^[1] and has the potential to phosphorylate other preformed nucleosides, specifically deoxyadenosine (dA) and deoxyguandine (dG), and convert them into their monophosphate forms." ^[2]^[3]^[4]^[5]

I would like to add a section for structure to later expand on the function of dCK (i.e. phosphorylation of Serine 74 on dCK changes the conformation of the enzyme from open to closed state). I will post an example of a paragraph I would like to add on a later date.

I would also like to add a section for the relationship between dCK and nucleotide biosynthesis (i.e. specifically dCK's function in the nucleoside salvage pathway and its relationship to the de novo pathway)

I would also like to add a section for the therapeutic implications and current prodrugs (i.e. gemcitabine, an FDA approved drug for cancer treatment that relies on dCK activity to function) mentioned in many papers in the further reading section to highlight dCK's potential as a target for pharmacological treatment in relation to cancer and how dCK functions in relation to cancer. However, I realize this may be a section that may need multiple revisions to ensure neutrality in perspective.

Please let me know if anyone has any suggestions or concerns about my outline for edits. Thank you Cmcheng28 (talk) 20:07, 1 November 2016 (UTC)[reply]

Peer Review[edit]

Hello Cmcheng28! I just finished reviewing your informative article on Deoxycytidine kinase. Below, are just some of my opinions on how you can improve on your Wiki edits.

Under the “Function” header, I would suggest to link the word “UTP” since it has its own wikipedia page and readers might like more information. Although you mention that dCK is clinically important towards the end of the section, are there any research articles that demonstrate its drug resistance and sensitivity, rather than just stating these? It would be nice to see some research experiments on the function and characteristics of dCK. Another possible header for this page is to include the discovery of dCK. Although I am not familiar with dCK, is there any enzymatic regulation that it has? If it does, it would also be nice to have an extra header for this topic.

Under “Nucleotide Biosynthesis” header, I would also suggest to link the word “salvage pathway” and explain the concept of the denovo pathway and how it relates to the downregulation of dCK. The “Therapeutic and Diagnostic Implications” is a very descriptive and clear summary for a reader, who is not familiar with dCK, to easily follow. Nevertheless, great job on your first Wiki article edit! Mmreynoso (talk) 07:55, 22 November 2016 (UTC)Mmreynoso[reply]

Reviewer #2[edit]

Your article is short, but is packed with information, and is very clearly written...just like an encyclopedia article should be. However, there's still a few things that can be improved. First of all, there's something weird going on with your first three references: it looks like something went wrong with inputting the information into the citation generator, and the links are broken (at least for me). You also seem to be a little low on references. I think 10 are required, so just cite a few of your "further reading" articles. I'm not sure why you chose not to in the first place: you can cite multiple sources for the same fact/statement and all it does is make your point stronger. Second, with a very specialized topic like dCK, it is very easy to get bogged down with terminology and make it very difficult for someone with little prior knowledge of biochemistry to understand what's going on. I'd consider fleshing out some of your short subsections with explanations of some of the terminology/biochemical concepts you mention (i.e. what a nucleoside triphosphate is, what de novo means, etc.). Just because you link a term to another Wikipedia page doesn't mean you don't have to explain what it means. Adding some explanations should also make your article a bit longer, which wouldn't hurt. Finally, I'd consider adding some kind of transitions to the ends/beginnings of each subsections, as this will help your article "flow" better and will make it really stand out. Hopefully this helps! Ricech (talk) 09:25, 22 November 2016 (UTC)[reply]

References[edit]

^ Nathanson, David A.; Armijo, Amanda L.; Tom, Michelle; Li, Zheng; Dimitrova, Elizabeth; Austin, Wayne R.; Nomme, Julian; Campbell, Dean O.; Ta, Lisa (2014-03-10). "Co-targeting of convergent nucleotide biosynthetic pathways for leukemia eradication". The Journal of Experimental Medicine. 211 (3): 473–486. doi:10.1084/jem.20131738. ISSN 0022-1007. PMC 3949575. PMID 24567448.
^ Hazra, Saugata; Szewczak, Andrzej; Ort, Stephan; Konrad, Manfred; Lavie, Arnon (2011-04-12). "Post-translational phosphorylation of serine 74 of human deoxycytidine kinase favors the enzyme adopting the open conformation making it competent for nucleoside binding and release". Biochemistry. 50 (14): 2870–2880. doi:10.1021/bi2001032. ISSN 1520-4995. PMC 3071448. PMID 21351740.
^ van der Wilt, C.L; Kroep, J.R; Loves, W.J.P; Rots, M.G; Groeningen, C.J Van; Kaspers, G.J; Peters, G.J. "Expression of deoxycytidine kinase in leukaemic cells compared with solid tumour cell lines, liver metastases and normal liver". European Journal of Cancer. 39 (5): 691–697. doi:10.1016/s0959-8049(02)00813-4.
^ Arnér, Elias S. J.; Eriksson, Staffan (1995-01-01). "Mammalian deoxyribonucleoside kinases". Pharmacology & Therapeutics. 67 (2): 155–186. doi:10.1016/0163-7258(95)00015-9.
^ Yamada, Yasukazu; Goto, Haruko; Ogasawara, Nobuaki (1983-11-22). "Purine nucleoside kinases in human T- and B-lymphoblasts". Biochimica et Biophysica Acta (BBA) - General Subjects. 761 (1): 34–40. doi:10.1016/0304-4165(83)90359-8.

Cmcheng28 (talk) 19:20, 1 November 2016 (UTC)[reply]

[:0-1] Nathanson, David A.; Armijo, Amanda L.; Tom, Michelle; Li, Zheng; Dimitrova, Elizabeth; Austin, Wayne R.; Nomme, Julian; Campbell, Dean O.; Ta, Lisa (2014-03-10). "Co-targeting of convergent nucleotide biosynthetic pathways for leukemia eradication". The Journal of Experimental Medicine. 211 (3): 473–486. doi:10.1084/jem.20131738. ISSN 0022-1007. PMC 3949575. PMID 24567448.

[2] Hazra, Saugata; Szewczak, Andrzej; Ort, Stephan; Konrad, Manfred; Lavie, Arnon (2011-04-12). "Post-translational phosphorylation of serine 74 of human deoxycytidine kinase favors the enzyme adopting the open conformation making it competent for nucleoside binding and release". Biochemistry. 50 (14): 2870–2880. doi:10.1021/bi2001032. ISSN 1520-4995. PMC 3071448. PMID 21351740.

[3] van der Wilt, C.L; Kroep, J.R; Loves, W.J.P; Rots, M.G; Groeningen, C.J Van; Kaspers, G.J; Peters, G.J. "Expression of deoxycytidine kinase in leukaemic cells compared with solid tumour cell lines, liver metastases and normal liver". European Journal of Cancer. 39 (5): 691–697. doi:10.1016/s0959-8049(02)00813-4.

[4] Arnér, Elias S. J.; Eriksson, Staffan (1995-01-01). "Mammalian deoxyribonucleoside kinases". Pharmacology & Therapeutics. 67 (2): 155–186. doi:10.1016/0163-7258(95)00015-9.

[5] Yamada, Yasukazu; Goto, Haruko; Ogasawara, Nobuaki (1983-11-22). "Purine nucleoside kinases in human T- and B-lymphoblasts". Biochimica et Biophysica Acta (BBA) - General Subjects. 761 (1): 34–40. doi:10.1016/0304-4165(83)90359-8.

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