Talk:Polysorbate 80

Data, Other Names sections

It looks like someone lifted this detail from some dense, esoteric, industry report. Can someone make this section relevant and meaningful to the casual reader? I have no idea what the 'critical micelle concentration' is, or what concentrations of 0.012 mM and 0.006mM mean.

Also, the list of side effects should probably be paired down, and should probably be under a more appropriate section title than 'Other Names'. Thanks 128.148.184.11 (talk) 01:17, 17 March 2009 (UTC)

Surfactants can associate to form soluble spherical structures (micelles) in aqueous solution from 10's of nanometers up to microns in diameter (size depends upon the size of the molecule, and thermodynamics). This formation is concentration dependent, and the lowest concentration at which the micelles form is called the "critical micelle concentration". The unit mM stands for milli-molar, or rather 10^-3 moles/Liter.Biotechscientist (talk) 11:27, 4 November 2010 (UTC)

CMC

According to the Calbiochem Detergents Manual (found here: http://www.emdbiosciences.com/SharedImages/TechnicalLiterature/1_Detbooklet_2001.pdf), the CMC of Tween 80 is 0.012 mM. This differs by an order of magnitude from the value presented. Upon review of the Journal of Pharmaceutical Sciences article, it seems a typo was made in entering the value into this article. Ian Glenn 03:14, 29 April 2007 (UTC)

Fixed. -- Ed (Edgar181) 14:07, 23 April 2008 (UTC)

I have read this entire paper, there was not a typo. They measured it. Page 1376 in this article thoroughly explains why CMC's are functions of the solution properties (e.g. pH, ionic strength). The differences stem from the fact that Calbiochem measured the CMC in pure water whereas the CMC's measured in this article also contained Albutropin.Biotechscientist (talk) 12:14, 4 November 2010 (UTC)

External link

The link in the second reference (to Vlasic) is dead. --129.116.200.92 21:27, 15 November 2007 (UTC)

I have removed it. -- Ed (Edgar181)

Chemical structure

The structure shown in the figure is incorrect and does not match the given molecular formula. The oleic acid chain is missing a single -CH₂- group. --24.14.237.138 (talk) 01:50, 23 April 2008 (UTC)

I have corrected the error in the image. Thanks for catching it. -- Ed (Edgar181) 14:01, 23 April 2008 (UTC)

The x + y + z should equal 80, for polysorbate 80. The x + y + z = 20 for polysorbate 20. Biotechscientist (talk) 11:19, 4 November 2010 (UTC)

Health effects

Studies have shown potentially serious health effects related to Tween 80. The anti HPV vaccine Gardasil contains Polysorbate 80, which is linked to infertility in mice,” noted Dee Nicholson, National Communications Director for Freedom in Canadian Health Care. [Nov 2007] Sleight of Handling: More Merck Magic Tricks With HPV Vaccine By Christopher C. Barr

A study published in December, 2005 discovered that Tween 80 can cause anaphylaxis, a sometimes fatal reaction characterized by a sharp drop in blood pressure, hives, and breathing difficulties. Researchers concluded that the severe reaction was not a typical allergic response characterized by the combination of IgE antibodies and the release of histamines; it was caused by a serious disruption that had occurred within the immune system.

In another study neonatal female rats were injected ip (0.1 ml/rat) with Tween 80 in 1, 5 or 10 percent aqueous solution on days 4-7 after birth. Treatment with Tween 80 accelerated maturation, prolonged the oestrus cycle, and induced persistent vaginal oestrus. The relative weight of the uterus and ovaries was decreased relative to the untreated controls. Squamous cell metaplasia of the epithelial lining of the uterus and cytological changes in the uterus were indicative of chronic oestrogenic stimulation. Ovaries were without corpora lutea, and had degenerative follicles.” ~ PMID: 8473002. Female lab rats injected with Tween 80 developed impaired sexual organs as well as premature development of their sexual organs. Institute of Preventive and Clinical Medicine, Limbová, Bratislava.

Previous studies by Gajdova et al. have shown that polysorbate 80 (also known as Tween 80) administered by intraperitoneal injection to neonatal female rats on days 4-7 after birth produced estrogenic effects including earlier vaginal opening, prolongation of the estrus cycle and persistent vaginal estrus. Some of these effects were evident many weeks after cessation of administration of polysorbate 80." [Gajdova et al - "Delayed effects of neonatal exposure to Tween 80 on female reproductive organs in rats." Food Chem Toxicol 31(3):183-90 (1993) Institute of Preventive and Clinical Medicine.

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I deleted the list of possible side effects, I found the (probable) source of this information, and it's a list of side effects that can be caused by drugs in general, not Tween 80 specific at all. —Preceding unsigned comment added by 213.31.11.80 (talk) 09:21, 27 March 2009 (UTC)

Thank you for deleting this, Polysorbate 80 has a long history of usage in parenteral drug formulation, and is on the GRAS (generally recognized as safe) list of the FDA. The concentrations used in these formulations is very small, 0.05-0.1% (w/v).Biotechscientist (talk) 11:32, 4 November 2010 (UTC)

Also it should be noted, that injecting a 2kg rat with 0.1mL of a 10%(w/v) solution is a dosage 600 times higher (based on g/kg) than the common parenteral formulation of a 0.5 mL, 0.05%(w/v) solution into a child (30kg).Biotechscientist (talk) 11:50, 4 November 2010 (UTC)

Possible link to Crohn's Disease?

Is someone better qualified than I able to follow up on this? —Preceding unsigned comment added by 203.16.28.241 (talk) 09:14, 18 October 2010 (UTC) I am not aware of any link to Crohn's disease. Crohn's disease is an autoimmune disorder characterized more recently by the over production of a powerful cytokine TNF-alpha. I do not know the biodistribution of Polysorbate 80 alone, however I would consider it unlikely to upregulate the production of TNF-alpha. —Preceding unsigned comment added by Biotechscientist (talk • contribs) 11:17, 4 November 2010 (UTC)