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{{User sandbox}}
{{User sandbox}}
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{{COI|date=May 2014}}
{{Multiple issues|{{Orphan|date=November 2014}}
{{Infobox non-profit
| name = Addgene
| image = [[File:Addgene_Logo.jpg|thumb]]
| caption =
| founder = Benjie Chen <br> Kenneth and Melina Fan
| type = [[Non-profit organization]], Biological resource center
| tax_id =
| registration_id =
| founded_date = 2004
| location = [[Cambridge, MA]], USA
| coordinates = <!-- {{Coord|LAT|LON|display=inline,title}} -->
| origins =
| key_people =
| area_served =
| products =
| services = Plasmid repository
| focus =
| mission =
| method =
| revenue =
| endowment =
| num_volunteers =
| num_employees =
| num_members =
| subsid =
| owner =
| motto =
| former name =
| homepage = <!-- {{URL|www.addgene.org}} -->
| dissolved =
| footnotes =
}}
}}
'''Morning Gloryville''' originally named Morning Glory, is a sober, drug-free morning [[rave]].


Morning Gloryville was founded in 2013 by Nico Thoemmes and Samantha Moyo.<ref name="How a morning rave could replace a workout at gym"/><ref name="Morning Glory: Swap The Gym For A 6:30am Rave To Boost Creativity Before Work"/> It was designed to be "fun... without the alcohol",<ref name="Wake up to breakfast raves">{{cite web|last1=Rubin|first1=Gareth|title=Wake up to breakfast raves – they’re a healthy way to start your working day|url=http://www.theguardian.com/uk-news/2014/nov/01/breakfast-raves-morning-gloryville-dawn-dancing|website=www.theguardian.com|publisher=The Guardian|accessdate=26 November 2014}}</ref> and an alternative to the morning gym.<ref name="Morning Glory: Swap The Gym For A 6:30am Rave To Boost Creativity Before Work">{{cite web|last1=Driscoll|first1=Brogan|title=Morning Glory: Swap The Gym For A 6:30am Rave To Boost Creativity Before Work|url=http://www.huffingtonpost.co.uk/2013/08/05/morning-glory-pre-work-rave-boost-creativity_n_3707097.html|website=http://www.huffingtonpost.co.uk|publisher=Huffington Post|accessdate=26 November 2014}}</ref> One of the aims is to "reengage people with a form of exercise" without the negative health impacts of drugs and alcohol.<ref name="Hate Exercise But Love To Dance? Try Conscious Clubbing">{{cite web|title=Hate Exercise But Love To Dance? Try Conscious Clubbing|url=http://www.mindbodygreen.com/0-14395/hate-exercise-but-love-to-dance-try-conscious-clubbing.html|website=www.mindbodygreen.com|publisher=Mind Body Green|accessdate=26 November 2014}}</ref>
'''Addgene''' is a non-profit, global [[plasmid]] repository. Addgene facilitates the exchange of genetic material between laboratories by storing plasmids and their associated cloning data. Plasmids can then be shipped to laboratories around the world for use in scientific research. Addgene also provides a free online database of plasmid cloning information and references, including lists of commonly used vector backbones, popular [[lentiviral]] plasmids, and [[molecular cloning]] protocols.
The original event occurs once a month in Shoreditch, East London.<ref name="How a morning rave could replace a workout at gym">{{cite web|title=How a morning rave could replace a workout at gym|url=http://www.bbc.co.uk/news/entertainment-arts-23525159|website=BBC news|accessdate=26 November 2014}}</ref><ref name="Rave your way to work! Clubbing-inspired exercise class helps early risers feel the burn by dancing to the beat BEFORE breakfast">{{cite web|last1=Waterlow|first1=Lucy|title=Rave your way to work! Clubbing-inspired exercise class helps early risers feel the burn by dancing to the beat BEFORE breakfast|url=http://www.dailymail.co.uk/femail/article-2411595/Morning-Glorys-Rave-Your-Way-Into-The-Day-exercise-class-gets-dancing-work.html|website=www.dailymail.co.uk|accessdate=26 November 2014}}</ref> Since then Morning Gloryville has expanded to 14 cities around the world.<ref name="Wake up to breakfast raves"/><ref name="Morning Gloryville website">{{cite web|title=Morning Gloryville locations|url=http://morninggloryville.com/|website=www.morninggloryville.com|accessdate=26 November 2014}}</ref><ref name="Early morning raves hit NYC"/> The event is not exclusively raving, but also includes yoga and massage.<ref name="Early morning raves hit NYC">{{cite web|title=Early morning raves hit NYC|url=http://www.cbsnews.com/news/morning-gloryville-early-morning-sober-raves-hit-nyc/|website=www.cbsnews.com|accessdate=26 November 2014}}</ref> Because the event is drug and alcohol free it is also popular with families and children.<ref name="It's Sunrise In London And Time For A Rave">{{cite web|title=It's Sunrise In London And Time For A Rave|url=http://www.npr.org/blogs/parallels/2014/08/12/338320513/its-sunrise-in-london-and-time-for-a-rave|website=www.npr.org|publisher=NPR|accessdate=26 November 2014}}</ref>


Notable DJ's including Basement Jaxx regularly play at Morning Gloryville.<ref name="Basement Jaxx Facebook Post about playing at Morning Gloryville">{{cite web|title=Basement Jaxx Facebook Post about playing at Morning Gloryville (26th Nov)|url=https://www.facebook.com/BasementJaxx|website=www.facebook.com|accessdate=26 November 2014}}</ref><ref name="Wake up to a breakfast rave">{{cite web|last1=Whittaker|first1=Adam|title=Wake up to a breakfast rave|url=http://www.stokesentinel.co.uk/Wake-breakfast-rave-Adam-Whittaker-Talking-Food/story-24387219-detail/story.html|website=http://www.stokesentinel.co.uk|accessdate=26 November 2014}}</ref> Morning Gloryville was also featured at Bestivle in Sept 2014.<ref name="Spotnight in the spotlight">{{cite web|title=Spotnight in the spotlight|url=http://blog.spotnightapp.com/post/93111031622/morning-gloryville-is-taking-over-our-cities-with|website=http://blog.spotnightapp.com|accessdate=26 November 2014}}</ref><ref name="Bestival 2014 Website photos">{{cite web|title=Bestival 2014 Website photos|url=http://photos.bestival.net/2014/8fd69c8a-34ee-11e4-8dc2-0a6b09a68d06|website=http://photos.bestival.net|accessdate=26 November 2014}}</ref>
==Description==
Addgene was founded in 2004, by Melina Fan, Ph.D., Kenneth Fan, and Benjie Chen, Ph.D., as a resource for scientists to easily store, search, and share plasmids for use in scientific and medical research.<ref>{{cite web|last=Scudellari|first=Megan|title=Sharing Made Easy|url=http://www.the-scientist.com/?articles.view/articleNo/32532/title/Sharing-Made-Easy/|work=The Scientist|accessdate=21 November 2012|year=2012}}</ref><ref>{{cite journal|last=Fan|first=M|author2=Tsai, J|author3= Chen, B|author4= Fan, K|author5= LaBaer, J|title=A central repository for published plasmids.|journal=Science|date=Mar 25, 2005|volume=307|issue=5717|pages=1877|pmid=15790830|doi=10.1126/science.307.5717.1877a}}</ref> Addgene's headquarters are located in [[Cambridge, MA]]. The plasmid collection is stored and shipped from Cambridge, MA, although a copy of each plasmid is also stored at a backup location in Rockville, MD.


==Conscious Clubbing==
It is free to deposit plasmids into Addgene's repository for distribution and to archive. As a non-profit, the organization covers the operating costs of maintaining and improving the collection by charging a nominal fee to scientists requesting plasmids.<ref>{{cite web|last=Kamens|first=J|title=Got any plasmids?|url=http://www.labtimes.org/labtimes/issues/lt2012/lt01/lt_2012_01_58_59.pdf|work=Lab Times|accessdate=21 November 2012|pages=58–59|year=2012}}</ref>
Morning Gloryville is also cited as creating the concept of “Conscious Clubbing”<ref name="Morning Glory: Swap The Gym For A 6:30am Rave To Boost Creativity Before Work"/><ref name="Early morning raves hit NYC"/><ref name="Conscious clubbing: could you rave sober?">{{cite web|last1=Jones|first1=Toni|title=Conscious clubbing: could you rave sober?|url=http://www.getthegloss.com/article/conscious-clubbing-could-you-rave-sober|website=http://www.getthegloss.com|accessdate=26 November 2014}}</ref>

This term can be used mean a variety of things. Clubs such as Raha use this to distinguish Non-profit-making monthly night clubs from commercial night clubs, as they can have more "ethical foundations" and they aim to "altering the concept of clubbing".<ref name="Forget drugs. City clubbers are on a new buzz now - consciousness">{{cite web|last1=Diski|first1=Chloe|title=Forget drugs. City clubbers are on a new buzz now - consciousness|url=http://www.theguardian.com/theobserver/2001/feb/11/features.review17|website=www.theguardian.com|accessdate=26 November 2014}}</ref>.
==Content==
Clubs such as Asleep At The Wheel use this term to identify this as an alternative club, which incorporates "live music, visuals, art, talks and poetry readings".<ref name="Forget drugs. City clubbers are on a new buzz now - consciousness"/>
'''Plasmid Repository'''

In 2014 Addgene's repository distributes 30,000 types of plasmids, deposited by 1,700 labs<ref>{{cite web|last=Baker|first=Monya|title=Repositories share key research tools|url=http://www.nature.com/news/repositories-share-key-research-tools-1.14511|publisher=Nature.com|accessdate=8 May 2014}}</ref> .
Addgene’s plasmid collection contains plasmids used for a variety of functions such as [[genome engineering]] (including CRISPRS), [[gene expression]], [[shRNA]] knockdown, viral-mediated gene delivery, detection of [[miRNA]] and promoter activity.

The plasmid collection includes:

* Genome engineering
** [[CRISPR]]s
** [[Transcription Activator-Like Effector Nuclease]] (TALEN) kits
** [[Zinc finger nuclease]] kits
* Empty backbones
** Species-specific expression
** Epitope tags
** Fusion proteins
** Selectable markers
** [[Fluorescent marker]]
* Viral vectors
** Retroviral/Lentiviral
** [[Adenoviral]]
** [[Adeno-associated virus|AAV]]
* [[cDNA]] expression
* shRNA expression


==Tools and Guides==

'''Molecular Biology Tools'''

[http://www.addgene.org/vector-database/ Vector Database]- A curated list of over 4,000 commonly used vector backbones, including relevant cloning information and bacterial growth conditions.

[http://www.addgene.org/analyze-sequence/ Sequence Analyzer]- A software tool developed by Addgene to create plasmid maps from sequences with annotated features and restriction sites.

[http://www.addgene.org/mol_bio_reference/ Molecular Biology Reference]- A collection of references for commonly used molecular biology reagents, such as primers, restriction enzymes, and antibiotic concentrations.

'''Plasmid Cloning Guides'''

[http://www.addgene.org/plasmid_reference/ Molecular Cloning Guides]- References to help scientists design their plasmid cloning experiments, including tutorials on restriction enzyme digestion and PCR-based cloning.

[http://www.addgene.org/plasmid_reference/index/ Molecular Cloning Protocols]- Specific protocols for a variety of plasmid cloning techniques, such as isolation of bacterial colonies, DNA purification by gel electrophoresis, and bacterial transformation.

==Collaborations==

Addgene collaborates with a number of institutes and consortiums to curate plasmid collections for specific research purposes. Examples of these collaborations include special collections from the [[Structural Genomics Consortium]], Zinc Finger Consortium, the Cell Migration Consortium and the [[Krüppel-like_factors|KLF]] collection.

On October 3, 2012, Addgene announced the launch of its partnership with [[The Michael J. Fox Foundation]].<ref name="MassHighTech">{{cite web|last=Resende|first=Patricia|title=Addgene partners with Michael J. Fox Foundation|url=http://www.masshightech.com/stories/2012/10/01/daily32-Addgene-partners-with-Michael-J-Fox-Foundation.html|work=Mass High Tech|accessdate=21 November 2012|date=3 October 2012}}</ref> The focus of the collaboration is to curate and distribute a large collection of plasmids for use in [[Parkinson’s Disease]] research. The plasmids are available to both academic and industry labs.

==Depositors==
Noteworthy depositors include:
*12 Nobel Prize winners; [[John Gurdon]], [[Shinya Yamanaka]], [[Bruce Beutler]], [[Mario Capecchi]], [[Andrew Fire]], [[Richard Axel]], [[Eric Wieschaus]], [[Phillip Sharp]], [[Robert Lefkowitz]], [[Martin Chalfie]], [[Roger Tsien]], and [[Johann Deisenhofer]].

*8 Breakthrough Prize in Life Sciences winners; [[Cori Bargmann]], [[David Botstein]], [[Lewis C. Cantley]], [[Hans Clevers]], [[Titia de Lange]], [[Bert Vogelstein]], [[Robert Weinberg]], and [[Shinya Yamanaka]].

==Electronic Material Transfer Agreements==

Addgene requires [[Material Transfer Agreement|Material Transfer Agreements]] (MTAs) to be in place for all materials transferred through Addgene to protect the intellectual property of plasmid depositors. Addgene has developed one of the first completely electronic systems for handling MTAs.<ref>{{cite journal|last=Herscovitch|first=Melanie|author2=Perkins, Eric|author3= Baltus, Andy|author4= Fan, Melina|title=Addgene provides an open forum for plasmid sharing|journal=Nature Biotechnology|date=10 April 2012|volume=30|issue=4|pages=316–317|doi=10.1038/nbt.2177}}</ref> By using the standard Universal Biological Material Transfer Agreement (UBMTA) and implementing electronic signatures, Addgene’s electronic MTA (eMTA) system expedites the approval process for plasmid orders.

==Awards==
Addgene has won a variety of awards for innovation and research including Mass Nonprofit Network Award for excellence in Innovations<ref>{{cite web|title=mass nonprofit network nominates addgene for an excellence award|url=http://www.prlog.org/12151032-mass-nonprofit-network-nominates-addgene-for-an-excellence-award.pdf|accessdate=9 May 2014}}</ref> <ref>{{cite web|title=mass non-profit, nonprofit award 2013 excellence in innovation finalists|url=http://www.massnonprofitnet.org/training-and-events/nonprofit-awareness-day/2013-excellence-awards/2013-excellence-in-innovation-finalists/|accessdate=9 May 2014}}</ref>, Cambridge award program 2014 Award in the Research & Development Laboratories<ref>{{cite web|title=cambridge recognition awarding|url=http://cambridge.cambridge.recognitionawarding.com/PressReleaseub.aspx?cc=DC7-EQ7D-W633|accessdate=9 May 2014}}</ref>, Mass Technology Leadership Award Finalist 2012<ref>{{cite web|title=Mass Tech announces finalist|url=http://blog.masstlc.org/2012/07/masstlc-announces-finalist-for-15th_20.html|accessdate=9 May 2014}}</ref>.


==References==
==References==
{{Reflist}}
{{reflist}}


==External links==
==Links==
* http://morninggloryville.com/
*[http://www.addgene.org Addgene official homepage]


[[:Category:Gene banks]]
[[Category:Nightclubs| ]]
[[:Category:Biotechnology databases]]
[[Category:Dance venues]]
[[:Category:Organizations based in Cambridge, Massachusetts]]
[[Category:Dance organizations]]
[[:Category:Non-profit organizations based in Massachusetts]]
[[Category:Rave culture in the United Kingdom]]
[[:Category:2004 establishments in Massachusetts]]

Revision as of 21:36, 1 December 2014

Morning Gloryville originally named Morning Glory, is a sober, drug-free morning rave.

Morning Gloryville was founded in 2013 by Nico Thoemmes and Samantha Moyo.[1][2] It was designed to be "fun... without the alcohol",[3] and an alternative to the morning gym.[2] One of the aims is to "reengage people with a form of exercise" without the negative health impacts of drugs and alcohol.[4] The original event occurs once a month in Shoreditch, East London.[1][5] Since then Morning Gloryville has expanded to 14 cities around the world.[3][6][7] The event is not exclusively raving, but also includes yoga and massage.[7] Because the event is drug and alcohol free it is also popular with families and children.[8]

Notable DJ's including Basement Jaxx regularly play at Morning Gloryville.[9][10] Morning Gloryville was also featured at Bestivle in Sept 2014.[11][12]

Conscious Clubbing

Morning Gloryville is also cited as creating the concept of “Conscious Clubbing”[2][7][13] This term can be used mean a variety of things. Clubs such as Raha use this to distinguish Non-profit-making monthly night clubs from commercial night clubs, as they can have more "ethical foundations" and they aim to "altering the concept of clubbing".[14]. Clubs such as Asleep At The Wheel use this term to identify this as an alternative club, which incorporates "live music, visuals, art, talks and poetry readings".[14]

References

  1. ^ a b "How a morning rave could replace a workout at gym". BBC news. Retrieved 26 November 2014.
  2. ^ a b c Driscoll, Brogan. "Morning Glory: Swap The Gym For A 6:30am Rave To Boost Creativity Before Work". http://www.huffingtonpost.co.uk. Huffington Post. Retrieved 26 November 2014. {{cite web}}: External link in |website= (help)
  3. ^ a b Rubin, Gareth. "Wake up to breakfast raves – they're a healthy way to start your working day". www.theguardian.com. The Guardian. Retrieved 26 November 2014.
  4. ^ "Hate Exercise But Love To Dance? Try Conscious Clubbing". www.mindbodygreen.com. Mind Body Green. Retrieved 26 November 2014.
  5. ^ Waterlow, Lucy. "Rave your way to work! Clubbing-inspired exercise class helps early risers feel the burn by dancing to the beat BEFORE breakfast". www.dailymail.co.uk. Retrieved 26 November 2014.
  6. ^ "Morning Gloryville locations". www.morninggloryville.com. Retrieved 26 November 2014.
  7. ^ a b c "Early morning raves hit NYC". www.cbsnews.com. Retrieved 26 November 2014.
  8. ^ "It's Sunrise In London And Time For A Rave". www.npr.org. NPR. Retrieved 26 November 2014.
  9. ^ "Basement Jaxx Facebook Post about playing at Morning Gloryville (26th Nov)". www.facebook.com. Retrieved 26 November 2014.
  10. ^ Whittaker, Adam. "Wake up to a breakfast rave". http://www.stokesentinel.co.uk. Retrieved 26 November 2014. {{cite web}}: External link in |website= (help)
  11. ^ "Spotnight in the spotlight". http://blog.spotnightapp.com. Retrieved 26 November 2014. {{cite web}}: External link in |website= (help)
  12. ^ "Bestival 2014 Website photos". http://photos.bestival.net. Retrieved 26 November 2014. {{cite web}}: External link in |website= (help)
  13. ^ Jones, Toni. "Conscious clubbing: could you rave sober?". http://www.getthegloss.com. Retrieved 26 November 2014. {{cite web}}: External link in |website= (help)
  14. ^ a b Diski, Chloe. "Forget drugs. City clubbers are on a new buzz now - consciousness". www.theguardian.com. Retrieved 26 November 2014.
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