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Biotium’s life science products predominantly include fluorescent reagents and consumables for cell or molecular biology, or protein analysis. In the early 2000’s the company was pioneering in offering nucleic acid stains that are safer for the user than other common options<ref name="Guzaev et al 2017">{{cite book |last1=Guzaev |first1=Mikhail |last2=Li |first2=Xue |last3=Park |first3=Candice |last4=Leung |first4=Wai-Yee |last5=Roberts |first5=Lori |title=Comparison of nucleic acid gel stains cell permeability, safety, and sensitivity of ethidium bromide alternatives |date=2017 |url=https://biotium.com/wp-content/uploads/2017/02/Gel-Stains-Comparison.pdf}}</ref><ref name="Haines 2015">{{cite journal |last1=Haines |first1=Alicia |last2=Tobe |first2=Shanan |last3=Kobus |first3=Hilton |last4=Linacre |first4=Adrian |title=Properties of nucleic acid staining dyes used in gel electrophoresis |journal=Electrophoresis |date=2015 |volume=36 |issue=6 |page=941-944 |url=https://biotium.com/wp-content/uploads/2017/02/Gel-Stains-Comparison.pdf}}</ref>, [https://en.wikipedia.org/wiki/GelRed GelRed®] and [https://en.wikipedia.org/wiki/GelGreen GelGreen®], and these products remain popular in both research and educational settings. EvaGreen® Dye is another popular molecular biology product developed by Biotium that offers an alternative to SYBR Green and has proven useful in PCR and HRM research as well as in molecular diagnostics and forensics protocols.<ref name="cheng 2013">{{cite journal |last1=Cheng |first1=Juhui |last2=Jiang |first2=Yonghou |last3=Rao |first3=Pinbin |last4=Wu |first4=Haigang |last5=Dong |first5=Qinfang |last6=Wu |first6=Zhiyi |last7=Ding |first7=Xianfeng |last8=Guo |first8=Jiangfeng |title=Development of a single-tube multiplex real-time PCR for detection and identification of five pathogenic targets by using melting-curve analysis with EvaGreen |journal=Archives of Virology 158 (2013): |date=2013 |volume=158 |page=379-386 |doi=https://doi.org/10.1007/s00705-012-1493-6 |pmid=23053522 |url=https://link.springer.com/article/10.1007/s00705-012-1493-6}}</ref><ref name="Eischeid 2011">{{cite journal |last1=Eischeid |first1=Anne |title=SYTO dyes and EvaGreen outperform SYBR Green in real-time PCR |journal=BMC research notes |date=2011 |volume=4 |page=1-5 |doi=https://doi.org/10.1186/1756-0500-4-263 |pmid=21798028 |url=https://link.springer.com/article/10.1186/1756-0500-4-263}}</ref><ref name="Mao, 2007">{{cite journal |last1=Mao |first1=Fei |last2=Leung |first2=Wai-Yee |last3=Xin |first3=Xing |title=Characterization of EvaGreen and the implication of its physicochemical properties for qPCR applications |journal=BMC biotechnology |date=2007 |volume=7 |page=1-16 |doi=https://doi.org/10.1186/1472-6750-7-76 |pmid=17996102 |url=https://link.springer.com/article/10.1186/1472-6750-7-76}}</ref>
Biotium’s life science products predominantly include fluorescent reagents and consumables for cell or molecular biology, or protein analysis. In the early 2000’s the company was pioneering in offering nucleic acid stains that are safer for the user than other common options<ref name="Guzaev et al 2017">{{cite book |last1=Guzaev |first1=Mikhail |last2=Li |first2=Xue |last3=Park |first3=Candice |last4=Leung |first4=Wai-Yee |last5=Roberts |first5=Lori |title=Comparison of nucleic acid gel stains cell permeability, safety, and sensitivity of ethidium bromide alternatives |date=2017 |url=https://biotium.com/wp-content/uploads/2017/02/Gel-Stains-Comparison.pdf}}</ref><ref name="Haines 2015">{{cite journal |last1=Haines |first1=Alicia |last2=Tobe |first2=Shanan |last3=Kobus |first3=Hilton |last4=Linacre |first4=Adrian |title=Properties of nucleic acid staining dyes used in gel electrophoresis |journal=Electrophoresis |date=2015 |volume=36 |issue=6 |page=941-944 |url=https://biotium.com/wp-content/uploads/2017/02/Gel-Stains-Comparison.pdf}}</ref>, [https://en.wikipedia.org/wiki/GelRed GelRed®] and [https://en.wikipedia.org/wiki/GelGreen GelGreen®], and these products remain popular in both research and educational settings. EvaGreen® Dye is another popular molecular biology product developed by Biotium that offers an alternative to SYBR Green and has proven useful in PCR and HRM research as well as in molecular diagnostics and forensics protocols.<ref name="cheng 2013">{{cite journal |last1=Cheng |first1=Juhui |last2=Jiang |first2=Yonghou |last3=Rao |first3=Pinbin |last4=Wu |first4=Haigang |last5=Dong |first5=Qinfang |last6=Wu |first6=Zhiyi |last7=Ding |first7=Xianfeng |last8=Guo |first8=Jiangfeng |title=Development of a single-tube multiplex real-time PCR for detection and identification of five pathogenic targets by using melting-curve analysis with EvaGreen |journal=Archives of Virology 158 (2013): |date=2013 |volume=158 |page=379-386 |doi=https://doi.org/10.1007/s00705-012-1493-6 |pmid=23053522 |url=https://link.springer.com/article/10.1007/s00705-012-1493-6}}</ref><ref name="Eischeid 2011">{{cite journal |last1=Eischeid |first1=Anne |title=SYTO dyes and EvaGreen outperform SYBR Green in real-time PCR |journal=BMC research notes |date=2011 |volume=4 |page=1-5 |doi=https://doi.org/10.1186/1756-0500-4-263 |pmid=21798028 |url=https://link.springer.com/article/10.1186/1756-0500-4-263}}</ref><ref name="Mao, 2007">{{cite journal |last1=Mao |first1=Fei |last2=Leung |first2=Wai-Yee |last3=Xin |first3=Xing |title=Characterization of EvaGreen and the implication of its physicochemical properties for qPCR applications |journal=BMC biotechnology |date=2007 |volume=7 |page=1-16 |doi=https://doi.org/10.1186/1472-6750-7-76 |pmid=17996102 |url=https://link.springer.com/article/10.1186/1472-6750-7-76}}</ref>

The company also developed its own line of CF® Dye [https://en.wikipedia.org/wiki/Fluorophore fluorophores] to offer certain benefits for various applications; CF® Dyes have been incorporated into a variety of kits, reactive options, and labeled antibodies.<ref name="Kist, Tarso B. Ledur. 2023" /><ref name="orres, E. (2020)">{{cite journal |last1=Torres, E. (2020). CF® Dyes. What started it all? Part 2. The Chemistry of Fluorescence. https://biotium.com/blog/cf-dyes-what-started-it-all-part-2-the-chemistry-of-fluorescence/ |first1=Eric |title=CF® Dyes. What started it all? Part 2. The Chemistry of Fluorescence |date=2020 |url=orres, E. (2020)https://biotium.com/blog/cf-dyes-what-started-it-all-part-2-the-chemistry-of-fluorescence/}}</ref> Many CF® Dyes have also now been shown by unsolicited third-party researchers to be compatible with various super-resolution microscopy methodologies (link https://en.wikipedia.org/wiki/Super-resolution_microscopy), including STORM (Bowler et al., 2019; Wang et al., 2022). A large selection of live cell fluorescent stains for subcellular structures or cellular processes, such as NucView® for real-time apoptosis detection (Cen et al., 2008; Alvero and Mor, 2021), are also offered.
The company also developed its own line of CF® Dye [https://en.wikipedia.org/wiki/Fluorophore fluorophores] to offer certain benefits for various applications; CF® Dyes have been incorporated into a variety of kits, reactive options, and labeled antibodies.<ref name="Kist, Tarso B. Ledur. 2023" /><ref name="Torres, E. (2020)">{{cite journal |last1=Torres, E. (2020). CF® Dyes. What started it all? Part 2. The Chemistry of Fluorescence. https://biotium.com/blog/cf-dyes-what-started-it-all-part-2-the-chemistry-of-fluorescence/ |first1=Eric |title=CF® Dyes. What started it all? Part 2. The Chemistry of Fluorescence |date=2020 |url=orres, E. (2020)https://biotium.com/blog/cf-dyes-what-started-it-all-part-2-the-chemistry-of-fluorescence/}}</ref> Many CF® Dyes have also now been shown by unsolicited third-party researchers to be compatible with various [https://en.wikipedia.org/wiki/Super-resolution_microscopy super-resolution microscopy] methodologies, including STORM. <ref name="Bowler, 2019">{{cite journal |last1=Bowler |first1=Mathew |last2=Kong |first2=Dong |last3=Sun |first3=Shufeng |last4=Nanjundappa |first4=Rashmi |last5=Evans |first5=Lauren |last6=Farmer |first6=Veronica |last7=Holland |first7=Andrew |last8=Mahjoub |first8=Moe R. |last9=Sui |first9=Haixin |last10=Jadranka |first10=Loncarek |title=High-resolution characterization of centriole distal appendage morphology and dynamics by correlative STORM and electron microscopy |journal=Nature communications |date=2019 |volume=10 |issue=1 |page=993 |doi=https://doi.org/10.1038/s41467-018-08216-4 |pmid=30824690 |url=https://www.nature.com/articles/s41467-018-08216-4}}</ref><ref name="Wang, 2022">{{cite journal |last1=Wang |first1=Bowen |last2=Xiong |first2=Michael |last3=Susanto |first3=Josephine |last4=Li |first4=Xue |last5=Leung |first5=Wai‐Yee |last6=Xu |first6=Ke |title=Transforming Rhodamine Dyes for (d) STORM Super‐Resolution Microscopy via 1, 3‐Disubstituted Imidazolium Substitution |journal=Angewandte Chemie International Edition |date=2022 |volume=61 |issue=9 |page=e202113612. |doi=https://doi.org/10.1002/anie.202113612 |pmid=34919772 |url=https://onlinelibrary.wiley.com/doi/abs/10.1002/anie.202113612}}</ref> A large selection of live cell fluorescent stains for subcellular structures or cellular processes, such as NucView® for real-time apoptosis detection, are also offered.<ref name="Cen 2008">{{cite journal |last1=Cen |first1=Hui |last2=Mao |first2=Fei |last3=Aronchik |first3=Ida |last4=Fuentes |first4=Rholinelle Joy |last5=Firestone |first5=Gary L. |title=DEVD‐NucView488: A novel class of enzyme substrates for real‐time detection of caspase‐3 activity in live cells |journal=The FASEB journal 22, no. 7 (2008): |date=2008 |volume=22 |issue=7 |page=2243-2252 |doi=https://doi.org/10.1096/fj.07-099234 |pmid=18263700 |url=https://faseb.onlinelibrary.wiley.com/doi/abs/10.1096/fj.07-099234}}</ref><ref name="Alvero and mor">{{cite book |last1=Alvero |first1=Ayesha B. |last2=Mor |first2=Gil G. |title=Alvero, Ayesha B., Gil G. Mor, and Alvero. Detection of Cell Death Mechanisms. Springer US, 2021. |date=2021 |publisher=Springer US |isbn=107161164X |pages=256 |url=https://link.springer.com/book/10.1007/978-1-0716-1162-3}}</ref>
Viability PCR (v-PCR) (link https://en.wikipedia.org/wiki/Viability_PCR) reagents are another specialty of Biotium, and they are currently the major global supplier of propidium monoazide (PMA) (link to and from https://en.wikipedia.org/wiki/Propidium_monoazide) for use in v-PCR. Biotium scientists also invented an alternative to PMA, named PMAxx™, that is more effective than PMA at eliminating false positives, PCR and LAMP amplification of dead cell DNA, in bacterial viability assays (Huang et al., 2021; Lv et al., 2021; Randazzo et al., 2018; Wen et al., 2022). Peer-reviewed publications have also shown that PMA and PMAxx™ are useful in monitoring viable bacteria in the environment and for assessing viral capsid integrity in DNA and RNA viruses (Oristo et al., 2018; Pedrosa de Macena et al., 2022).

[https://en.wikipedia.org/wiki/Viability_PCR Viability PCR (v-PCR)] reagents are another specialty of Biotium, and they are currently the major global supplier of [https://en.wikipedia.org/wiki/Propidium_monoazide propidium monoazide (PMA)] for use in v-PCR. Biotium scientists also invented an alternative to PMA, named PMAxx™, that is more effective than PMA at eliminating false positives, PCR and LAMP amplification of dead cell DNA, in bacterial viability assays.<ref name="Lv, 2021">{{cite journal |last1=Lv |first1=Xinrui |last2=Gu |first2=Xiaokui |last3=Wang |first3=Li |last4=He |first4=Xiaoxin |last5=He |first5=Chunai |last6=Zhang |first6=Jingfeng |last7=Zhao |first7=Lichao |title=Rapid and absolute quantification of VBNC Cronobacter sakazakii by PMAxx combined with single intact cell droplet digital PCR in infant foods |journal=LWT |date=2021 |volume=145 |page=111388 |doi=https://doi.org/10.1016/j.lwt.2021.111388 |url=https://www.sciencedirect.com/science/article/abs/pii/S0023643821005417}}</ref><ref name="Huang, 2021">{{cite journal |last1=Huang |first1=Tiantian |last2=Shi |first2=Yaoqiang |last3=Zhang |first3=Jinyang |last4=J |first4=Huang |last5=Han |first5=Qinqin |last6=Xia |first6=Xue-shan |last7=Zhang |first7=A-Mei |last8=Song |first8=Yuzhu Song |title=Rapid and simultaneous detection of five, viable, foodborne pathogenic bacteria by photoinduced PMAxx-coupled multiplex PCR in fresh juice |journal=Foodborne Pathogens and Disease |date=2021 |volume=18 |issue=9 |page=640-646 |doi=https://doi.org/10.1089/fpd.2020.29 |pmid=34292761 |url=https://www.liebertpub.com/doi/abs/10.1089/fpd.2020.2909}}</ref><ref name="Randazzo, 2018">{{cite journal |last1=Randazzo |first1=Walter |last2=Piqueras |first2=Joaquín |last3=Rodríguez‐Díaz |first3=Jesús |last4=Aznar |first4=Rosa |last5=Sánchez |first5=G. |title=Improving efficiency of viability‐qPCR for selective detection of infectious HAV in food and water samples. |journal=Journal of applied microbiology |date=2018 |volume=124 |issue=4 |page=958-964 |doi=https://doi.org/10.1111/jam.13519 |pmid=28649706 |url=https://academic.oup.com/jambio/article-abstract/124/4/958/6714013}}</ref><ref name="Wen 2022">{{cite journal |last1=Wen |first1=Yuanyi |last2=Tan |first2=Youjiang |last3=Zhao |first3=Lichao |last4=Lv |first4=Xinrui |last5=Lin |first5=Li |last6=Liang |first6=Dezhi |last7=Wang |first7=Li |title=Rapid on-site detection of viable Escherichia coli O157: H7 in lettuce using immunomagnetic separation combined with PMAxx-LAMP and nucleic acid lateral flow strip |journal=Microchemical Journal |date=2022 |volume=178 |page=107348 |doi=https://doi.org/10.1016/j.microc.2022.107348 |url=https://www.sciencedirect.com/science/article/abs/pii/S0026265X2200176X}}</ref> Peer-reviewed publications have also shown that PMA and PMAxx™ are useful in monitoring viable bacteria in the environment and for assessing viral capsid integrity in DNA and RNA viruses.<ref name="Oristo 2018">{{cite journal |last1=Oristo |first1=S. |last2=Lee |first2=H.J. |last3=Maunula |first3=L. |title=Performance of pre‐RT‐qPCR treatments to discriminate infectious human rotaviruses and noroviruses from heat‐inactivated viruses: applications of PMA/PMAxx, benzonase and RNase |journal=Journal of applied microbiology |date=2018 |volume=124 |issue=4 |page=1008-1016 |doi=https://doi.org/10.1111/jam.13737 |pmid=29464825 |url=https://academic.oup.com/jambio/article-abstract/124/4/1008/6714018}}</ref><ref name="Pedrosa, 2022">{{cite journal |last1=Pedrosa de Macena |first1=Lorena da Graça |last2=Simone de Oliveira Pereira |first2=Joseane |last3=César Ferreira |first3=Fernando |last4=Gonçalves Maranhão |first4=Adriana |last5=Maria Lanzarini |first5=Natália |last6=Miagostovich |first6=Marize Pereira |title=Quantification of infectious Human mastadenovirus in environmental matrices using PMAxx-qPCR |journal=Brazilian Journal of Microbiology |date=2022 |volume=53 |issue=3 |page=1465-1471 |doi=https://doi.org/10.1007/s42770-022-00775-5 |pmid=35666431 |url=https://link.springer.com/article/10.1007/s42770-022-00775-5}}</ref>
Biotium also sells select equipment for use with its reagents as well as a variety of accessory consumables. Their sales platforms include direct-to-customer sales for domestic customers and numerous domestic and international distributors.
Biotium also sells select equipment for use with its reagents as well as a variety of accessory consumables. Their sales platforms include direct-to-customer sales for domestic customers and numerous domestic and international distributors.


==== History ====
==== History ====


Biotium was founded in 2001, by Fei Mao Ph.D., a graduate of the University of Oregon (“Biotech Entrepreneur to Speak at Distinguished Alumni Event,” 2017), and co-founder Vivien Chen. Since then, Biotium researchers have developed over 30 patented technologies. Many of these patented technologies have been licensed to leading life science technology companies worldwide. The company is currently headquartered in Fremont, California.
Biotium was founded in 2001, by Fei Mao Ph.D., a graduate of the University of Oregon<ref name="Biotech entrepreneur">{{cite news |last1=Staff reporter |title=Biotech entrepreneur to speak at distinguished alumni event |url=https://news.uoregon.edu/content/biotech-entrepreneur-speak-distinguished-alumni-event |date=2017}}</ref>, and co-founder Vivien Chen MBA. Since then, Biotium researchers have developed over 30 patented technologies. Many of these patented technologies have been licensed to leading life science technology companies worldwide. The company is currently headquartered in Fremont, California.

Revision as of 19:55, 23 July 2024

Biotium Inc. is a privately held American life science reagent manufacturer and supplier founded in 2001. Biotium specializes in providing high-quality fluorescent reagents for use in microscopy, flow cytometry, or molecular biology applications.[1][2][3] The company is located in the Bay Area in a facility that currently receives 80% of its power from solar energy. Biotium’s collaborative R&D teams include chemists and biologists who act synergistically to keep the company at the forefront of fluorescent dye design, applying chemistry-based principles toward producing solutions for unmet challenges in life science and medical research.

Business segments

Biotium’s life science products predominantly include fluorescent reagents and consumables for cell or molecular biology, or protein analysis. In the early 2000’s the company was pioneering in offering nucleic acid stains that are safer for the user than other common options[4][5], GelRed® and GelGreen®, and these products remain popular in both research and educational settings. EvaGreen® Dye is another popular molecular biology product developed by Biotium that offers an alternative to SYBR Green and has proven useful in PCR and HRM research as well as in molecular diagnostics and forensics protocols.[6][7][8]

The company also developed its own line of CF® Dye fluorophores to offer certain benefits for various applications; CF® Dyes have been incorporated into a variety of kits, reactive options, and labeled antibodies.[2][9] Many CF® Dyes have also now been shown by unsolicited third-party researchers to be compatible with various super-resolution microscopy methodologies, including STORM. [10][11] A large selection of live cell fluorescent stains for subcellular structures or cellular processes, such as NucView® for real-time apoptosis detection, are also offered.[12][13]

Viability PCR (v-PCR) reagents are another specialty of Biotium, and they are currently the major global supplier of propidium monoazide (PMA) for use in v-PCR. Biotium scientists also invented an alternative to PMA, named PMAxx™, that is more effective than PMA at eliminating false positives, PCR and LAMP amplification of dead cell DNA, in bacterial viability assays.[14][15][16][17] Peer-reviewed publications have also shown that PMA and PMAxx™ are useful in monitoring viable bacteria in the environment and for assessing viral capsid integrity in DNA and RNA viruses.[18][19] Biotium also sells select equipment for use with its reagents as well as a variety of accessory consumables. Their sales platforms include direct-to-customer sales for domestic customers and numerous domestic and international distributors.

History

Biotium was founded in 2001, by Fei Mao Ph.D., a graduate of the University of Oregon[20], and co-founder Vivien Chen MBA. Since then, Biotium researchers have developed over 30 patented technologies. Many of these patented technologies have been licensed to leading life science technology companies worldwide. The company is currently headquartered in Fremont, California.

  1. ^ Connon, Catherine Cupples (2023). Forensic DNA Analysis: Methods and Protocols (Vol. 2685 ed.). Springer Nature. p. 440. ISBN 1071632949.
  2. ^ a b Kist, Tarso; Ledur, B. (2023). Fluorescent Dye Labels and Stains: A Database of Photophysical Properties. John Wiley & Sons. p. 480. ISBN 978-1-119-83513-4.
  3. ^ Goetz, Christine; Hammerbeck, Christopher; Bonnevier, Jody (2018). Flow cytometry basics for the non-expert (Vol. 1 ed.). Cham, Switzerland: Springer. p. 240. ISBN 331998070X.
  4. ^ Guzaev, Mikhail; Li, Xue; Park, Candice; Leung, Wai-Yee; Roberts, Lori (2017). Comparison of nucleic acid gel stains cell permeability, safety, and sensitivity of ethidium bromide alternatives (PDF).
  5. ^ Haines, Alicia; Tobe, Shanan; Kobus, Hilton; Linacre, Adrian (2015). "Properties of nucleic acid staining dyes used in gel electrophoresis" (PDF). Electrophoresis. 36 (6): 941-944.
  6. ^ Cheng, Juhui; Jiang, Yonghou; Rao, Pinbin; Wu, Haigang; Dong, Qinfang; Wu, Zhiyi; Ding, Xianfeng; Guo, Jiangfeng (2013). "Development of a single-tube multiplex real-time PCR for detection and identification of five pathogenic targets by using melting-curve analysis with EvaGreen". Archives of Virology 158 (2013):. 158: 379-386. doi:https://doi.org/10.1007/s00705-012-1493-6. PMID 23053522. {{cite journal}}: Check |doi= value (help); External link in |doi= (help)CS1 maint: extra punctuation (link)
  7. ^ Eischeid, Anne (2011). "SYTO dyes and EvaGreen outperform SYBR Green in real-time PCR". BMC research notes. 4: 1-5. doi:https://doi.org/10.1186/1756-0500-4-263. PMID 21798028. {{cite journal}}: Check |doi= value (help); External link in |doi= (help)
  8. ^ Mao, Fei; Leung, Wai-Yee; Xin, Xing (2007). "Characterization of EvaGreen and the implication of its physicochemical properties for qPCR applications". BMC biotechnology. 7: 1-16. doi:https://doi.org/10.1186/1472-6750-7-76. PMID 17996102. {{cite journal}}: Check |doi= value (help); External link in |doi= (help)
  9. ^ Torres, E. (2020). CF® Dyes. What started it all? Part 2. The Chemistry of Fluorescence. https://biotium.com/blog/cf-dyes-what-started-it-all-part-2-the-chemistry-of-fluorescence/, Eric (2020). [orres, E. (2020)https://biotium.com/blog/cf-dyes-what-started-it-all-part-2-the-chemistry-of-fluorescence/ "CF® Dyes. What started it all? Part 2. The Chemistry of Fluorescence"]. {{cite journal}}: Check |url= value (help); Cite journal requires |journal= (help); External link in |last1= (help)CS1 maint: numeric names: authors list (link)
  10. ^ Bowler, Mathew; Kong, Dong; Sun, Shufeng; Nanjundappa, Rashmi; Evans, Lauren; Farmer, Veronica; Holland, Andrew; Mahjoub, Moe R.; Sui, Haixin; Jadranka, Loncarek (2019). "High-resolution characterization of centriole distal appendage morphology and dynamics by correlative STORM and electron microscopy". Nature communications. 10 (1): 993. doi:https://doi.org/10.1038/s41467-018-08216-4. PMID 30824690. {{cite journal}}: Check |doi= value (help); External link in |doi= (help)
  11. ^ Wang, Bowen; Xiong, Michael; Susanto, Josephine; Li, Xue; Leung, Wai‐Yee; Xu, Ke (2022). "Transforming Rhodamine Dyes for (d) STORM Super‐Resolution Microscopy via 1, 3‐Disubstituted Imidazolium Substitution". Angewandte Chemie International Edition. 61 (9): e202113612. doi:https://doi.org/10.1002/anie.202113612. PMID 34919772. {{cite journal}}: Check |doi= value (help); External link in |doi= (help)
  12. ^ Cen, Hui; Mao, Fei; Aronchik, Ida; Fuentes, Rholinelle Joy; Firestone, Gary L. (2008). "DEVD‐NucView488: A novel class of enzyme substrates for real‐time detection of caspase‐3 activity in live cells". The FASEB journal 22, no. 7 (2008):. 22 (7): 2243-2252. doi:https://doi.org/10.1096/fj.07-099234. PMID 18263700. {{cite journal}}: Check |doi= value (help); External link in |doi= (help)CS1 maint: extra punctuation (link)
  13. ^ Alvero, Ayesha B.; Mor, Gil G. (2021). Alvero, Ayesha B., Gil G. Mor, and Alvero. Detection of Cell Death Mechanisms. Springer US, 2021. Springer US. p. 256. ISBN 107161164X.
  14. ^ Lv, Xinrui; Gu, Xiaokui; Wang, Li; He, Xiaoxin; He, Chunai; Zhang, Jingfeng; Zhao, Lichao (2021). "Rapid and absolute quantification of VBNC Cronobacter sakazakii by PMAxx combined with single intact cell droplet digital PCR in infant foods". LWT. 145: 111388. doi:https://doi.org/10.1016/j.lwt.2021.111388. {{cite journal}}: Check |doi= value (help); External link in |doi= (help)
  15. ^ Huang, Tiantian; Shi, Yaoqiang; Zhang, Jinyang; J, Huang; Han, Qinqin; Xia, Xue-shan; Zhang, A-Mei; Song, Yuzhu Song (2021). "Rapid and simultaneous detection of five, viable, foodborne pathogenic bacteria by photoinduced PMAxx-coupled multiplex PCR in fresh juice". Foodborne Pathogens and Disease. 18 (9): 640-646. doi:https://doi.org/10.1089/fpd.2020.29. PMID 34292761. {{cite journal}}: Check |doi= value (help); External link in |doi= (help)
  16. ^ Randazzo, Walter; Piqueras, Joaquín; Rodríguez‐Díaz, Jesús; Aznar, Rosa; Sánchez, G. (2018). "Improving efficiency of viability‐qPCR for selective detection of infectious HAV in food and water samples". Journal of applied microbiology. 124 (4): 958-964. doi:https://doi.org/10.1111/jam.13519. PMID 28649706. {{cite journal}}: Check |doi= value (help); External link in |doi= (help)
  17. ^ Wen, Yuanyi; Tan, Youjiang; Zhao, Lichao; Lv, Xinrui; Lin, Li; Liang, Dezhi; Wang, Li (2022). "Rapid on-site detection of viable Escherichia coli O157: H7 in lettuce using immunomagnetic separation combined with PMAxx-LAMP and nucleic acid lateral flow strip". Microchemical Journal. 178: 107348. doi:https://doi.org/10.1016/j.microc.2022.107348. {{cite journal}}: Check |doi= value (help); External link in |doi= (help)
  18. ^ Oristo, S.; Lee, H.J.; Maunula, L. (2018). "Performance of pre‐RT‐qPCR treatments to discriminate infectious human rotaviruses and noroviruses from heat‐inactivated viruses: applications of PMA/PMAxx, benzonase and RNase". Journal of applied microbiology. 124 (4): 1008-1016. doi:https://doi.org/10.1111/jam.13737. PMID 29464825. {{cite journal}}: Check |doi= value (help); External link in |doi= (help)
  19. ^ Pedrosa de Macena, Lorena da Graça; Simone de Oliveira Pereira, Joseane; César Ferreira, Fernando; Gonçalves Maranhão, Adriana; Maria Lanzarini, Natália; Miagostovich, Marize Pereira (2022). "Quantification of infectious Human mastadenovirus in environmental matrices using PMAxx-qPCR". Brazilian Journal of Microbiology. 53 (3): 1465-1471. doi:https://doi.org/10.1007/s42770-022-00775-5. PMID 35666431. {{cite journal}}: Check |doi= value (help); External link in |doi= (help)
  20. ^ Staff reporter (2017). "Biotech entrepreneur to speak at distinguished alumni event".
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