Introduction to genetics

A section of DNA, the sequence of the plate-like units (nucleotides) in the center carries information.

This article is a non-technical introduction to the subject. For the main encyclopedia article, see Genetics.

Genetics studies inheritance in living things. Living organisms inherit many of the features of their ancestors – for example, children usually look and act like other people in their family. Genetics tries to identify which features are inherited, and work out the details of how these features are passed from generation to generation.

In genetics, a feature of an organism is called a "trait". A trait can be a feature of an organism's physical appearance, for example, a person's eye color, height or weight. Traits are often inherited, for example tall and thin people tend to have tall and thin children. However, many traits come from the interaction between inherited features and the environment. For example a person might inherit the tendency to be tall, but if there is very little food where they live and they are poorly nourished, they will still be short. The way genetics and environment interact in producing a trait can be complicated, which is important in medical genetics. The chances of somebody dying of cancer or heart disease seem to depend on both their family history and their lifestyle.

Genetic information is carried by a long molecule called DNA and this DNA is copied and inherited across generations. Traits are carried in DNA as instructions for constructing and operating an organism. These instructions are contained in segments of DNA called genes. DNA is made of a sequence of simple units, with the order of these units spelling out instructions in the genetic code. This is similar to the orders of letters spelling out words. The organism "reads" the sequence of these units and decodes the instruction.

Not all the genes for a particular instruction are exactly the same. Different forms of one type of gene are called different alleles of that gene. As an example, one allele of a gene for hair color could carry the instruction to produce a lot of the pigment in black hair, while a different allele could give a garbled version of this instruction, so that no pigment is produced and the hair is white. Mutations are events that change the sequence of a gene and therefore create a new allele. Mutations can produce a new trait, such as turning an allele for black hair into an allele for white hair. The appearance of new traits is important in evolution.

Inheritance in biology

Genes and inheritance

Genes are inherited as units, with parents dividing out their genes to their offspring. You can think of this process like mixing two hands of cards, shuffling them, and then dealing them out again. Humans have two copies of each of their genes and when people reproduce they make copies of their genes in eggs or sperm, but only put in one copy of each type of gene. An egg then joins with a sperm to give a child with a new set of genes. This child will have the same number of genes as its parents but for any gene one of their two copies will come from the father, and one from the mother.

The effects of this mixing depends on the types (the alleles) of the gene you are interested in. If the father has two alleles specifying green eyes, and the mother has two alleles specifying brown eyes, all their children will get two alleles giving different instructions, one for green eyes and one for brown. The eye color of these children depends on how these alleles work together. If one allele overrides the instructions from another, it is called the dominant allele, and the allele that is overridden is called the recessive allele. In the case of a daughter with both green and brown alleles, brown is dominant and she ends up with brown eyes.

Green eyes are a recessive trait.

However, the green eye color allele is still there in this brown-eyed girl, it just doesn't show. This is a difference between what you see on the surface (the set of traits of an organism, also called its phenotype) and the genes in this organism's chromosomes (its genotype). In this example you can call the brown allele "B" and the green allele "g". (It is normal to write dominant alleles with capital letters and recessive ones with lower-case letters.) The brown-eyed daughter has the "brown eye phenotype" but her genotype is Bg, with one copy of the B allele, and one of the g allele.

Now imagine that this woman grows up and has children with a brown-eyed man who also has a Bg genotype. Her eggs will be a mixture of two types, one sort containing the B allele, and one sort the g allele. Similarly, her partner will produce a mix of two types of sperm containing one or the other of the two alleles. Now, when the alleles are mixed up in the offspring, these children have a chance of getting either brown or green eyes, since they could get a genotype of BB = brown eyes, Bg = brown eyes or gg = green eyes. In this generation, there is therefore a chance of the recessive allele showing itself in the phenotype of the children - some of them will have green eyes like their grandfather. The inheritance of eye color can be a bit more complicated than this, but all cases follow the same general principles.

However, many traits are inherited in a more complicated way than the example above. This can happen when there are several genes involved, each of which contributes a small part to the end result. Tall people tend to have tall children because their children get a package of many alleles that each contribute a bit to how much they grow. However, there are not clear groups of "short people" and "tall people", like there are groups of people with brown or green eyes. This is because of the large number of genes involved, this makes the trait very variable and people are many different heights. Inheritance can also be complicated when the trait depends on the interaction between genetics and the environment. This is quite common, for example, if a child does not eat enough nutritious food this will not change traits like eye color, but it could certainly stunt their growth.

Inherited diseases

Some diseases are hereditary and run in families; others, such as infectious diseases, are caused by the environment. However, many disorders are caused by a combination of hereditary and environmental factors.

Diseases that are caused by a single allele of a gene, and are inherited in a simple way in families are called genetic disorders. These include diseases like Huntington's disease, Cystic fibrosis or Duchenne muscular dystrophy. Cystic fibrosis, for example, is caused by mutations in a single gene called CFTR and is inherited as a recessive trait. Other diseases are influenced by genetics, but which alleles a person gets from their parents only change their risk of getting a disease. Most of these diseases are inherited in a complex way, with either multiple genes involved, or both genes and the environment being important.

As an example, the risk of breast cancer is 50 times higher in the families most at risk, compared to the families least at risk. This variation is probably due to a large number of alleles, each of which change the risk a little bit. Several of the genes involved have been identified, such as BRCA1 and BRCA2, but not all of them. However, although some of the risk is genetic, being overweight, drinking a lot of alcohol or not exercising, all increase the risk of this cancer. Any one woman's risk of breast cancer is therefore the result of a large number of alleles and her environment, so is very hard to predict accurately.

How genes work

Genes make proteins

The function of genes is to provide the information needed to make molecules called proteins in cells. Cells are the smallest independent parts of organisms: the human body contains many millions of cells, while very small organisms like bacteria are just a single cell. A cell is like a miniature and very complex factory that can make all the parts needed to produce a copy of itself, which happens when cells divide. There is a simple division of labor in cells - genes give instructions and proteins carry out these instructions, tasks like building a new copy of a cell, or repairing damage. Each type of protein is a specialist that only does one job, so if a cell needs to do something new, it must make a new protein to do this job. Similarly, if a cell needs to do something faster or slower than before, it makes more or less of the protein responsible. Genes tell cells what to do by telling them which proteins to make and in what amounts.

Genes are expressed by being transcribed into RNA, and this RNA then translated into protein.

Proteins are made of a chain of 20 different types of amino acids. This chain folds up into a compact shape, rather like an untidy ball of rope. The shape of the protein is determined by the sequence of amino acids along its chain and it is this shape that, in turn, determines what the protein will do. For example, some proteins have depressions in their surface that perfectly match another molecule, allowing the protein to bind to this molecule very tightly. Other proteins are enzymes, which are like tiny machines that can alter other molecules.

In DNA information is held in the sequence of the repeating units along the DNA chain. These units are four types of nucleotides (A,T,G and C) and the sequence of nucleotides stores information in an alphabet called the genetic code. When a gene is read by a cell the DNA sequence is copied into a very similar molecule called RNA (this process is called transcription). The RNA copy is then fed through a structure called a ribosome, which translates the sequence of nucleotides in the RNA into the correct sequence of amino acids and joins these amino acids together to make a complete protein chain. The new protein then folds up into its active form. The process of moving information from the language of DNA into the language of amino acids is called translation.

DNA replication. DNA is unwound and nucleotides are matched to make two new strands.

If the sequence of the nucleotides in a gene changes, then sequence of the amino acids in the protein it produces may also change - if part of a gene is deleted, the protein produced will be shorter and may not work any more. This is the reason why different alleles of a gene can have different effects in an organism. As an example, hair color depends on how much of a dark substance called melanin is put into the hair as it grows. If a person has a normal set of the genes involved in making melanin, they make all the proteins needed and they grow dark hair. However, if the alleles for a particular protein have different sequences and produce proteins that do not do the job correctly, no melanin will be produced and the hair will be white. This condition is called albinism and the person suffering from it is called an albino.

Copies of genes are inherited

When genes are passed from a parent to a child they are copied - the parent keeps all the original copies and just passes on the new copies to their offspring. Genes are also copied each time a cell divides into two new cells. The process that copies DNA is called DNA replication.

DNA can be copied very easily and accurately because each piece of DNA contains two copies of its information. This is because DNA is made of two strands that pair together like the two sides of a zipper. The nucleotides are in the center, like the teeth in the zipper, and pair up to hold the two strands together. Importantly, the four different sorts nucleotides are different shapes, so in order for the strands to close up properly, an A nucleotide must go opposite a T nucleotide, and a G opposite a C. This exact pairing is called base pairing.

When DNA is copied, the two strands of the old DNA are pulled apart and proteins move along each of the two single strands pairing up new nucleotide units and then zipping the strands closed. This produces two new pieces of DNA, each containing one strand from the old DNA and one newly-made strand.

However, this process isn't perfect and sometimes the proteins will make mistakes and put the wrong nucleotide into the strand they are building. This causes a change in the sequence of that gene. These changes in DNA sequence are called mutations. Mutations produce new alleles of genes. Sometimes these changes stop the gene from working properly, like the melanin genes discussed above. In other cases these mutations can change what the gene does or even let it do its job a little better than before. These mutations and their effects on the traits of organisms are one of the causes of evolution.

Genes and evolution

Further information: [[:Introduction to evolution]]

Mice with different coat colors.

A population of organisms evolves when an inherited trait becomes more or less common over time. For instance, all the mice living on an island would be a single population of mice. If over a few generations of breeding, white mice went from being rare, to being a large part of this population, then the coat color of these mice would be evolving. In terms of genetics, evolution happens when an allele becomes more or less common in a population. This is called a change in allele frequency - such as an increase in the frequency of the allele for white fur.

Alleles become more or less common either just by chance (in a process called genetic drift), or through natural selection. In natural selection if an allele makes it more likely that an organism will survive and reproduce, then over time this allele will become more common. Equally, if an allele is harmful, natural selection will cause it to become less common. For example, if the island was getting colder each year and was covered with snow for much of the time, then the allele for white fur would become advantageous, since it would make mice harder to see against the snow. Fewer of the white mice would be eaten by predators, so over time white mice would out-compete mice with dark fur. White fur alleles would become more common, and dark fur alleles would become more rare.

Mutations create new alleles, these alleles have new DNA sequences and can produce proteins with new properties. So if an island was populated entirely by black mice, mutations could happen creating alleles for white fur. The combination of mutations creating new alleles at random, and natural selection picking out those which are useful, causes adaptation. This is when organisms change in ways that help them to survive and reproduce.

Genetic engineering

Since traits come from the genes in a cell, if you put a new piece of DNA into a cell, this can produce a new trait. This transfer of genes is how genetic engineering works. For example, crop plants can be given a gene from an arctic fish, so they produce an antifreeze protein in their leaves. This can help prevent frost damage. Other genes that are can be put into crops include a natural insecticide from the bacteria Bacillus thuringiensis that kills insects that eat the plants, but is harmless to people. In these plants the new genes are put into the plant before it is grown, so the genes will be in every part of the plant, including its seeds. This means that the plant's offspring will inherit the new genes and has lead to concern about the spread of new traits into wild plants.

The same kind of technology used in genetic engineering is also being developed to treat people with genetic disorders in an experimental medical technique called gene therapy. However, here the new gene is put in after the person has grown up and become ill, so any new gene will not be inherited by their children. Gene therapy works by trying to replace the allele that causes the disease with an allele that will work properly.

Further reading

• Jones, Steve (2000). The Language of the Genes. Flamingo. ISBN 0-00-655243-9. (Aventis Prize winner)
• Schwartz, James (2008). In Pursuit of the Gene: From Darwin to DNA. Harvard University Press. ISBN 0-67-402670-5.
• Hamer, Dean and Copeland, Peter (1999). Living with Our Genes: Why They Matter More Than You Think. Anchor. ISBN 0-38-548584-0.{{cite book}}: CS1 maint: multiple names: authors list (link)

External links

General

• Introduction to Genetics, University of Utah

DNA and genes

• Animated guide to cloning
• Genetics NCBI, A Science Primer

Evolution

• Understanding Evolution: your one-stop source for information on evolution (web resource), The University of California Museum of Paleontology, Berkeley, retrieved 2008-01-24

Interactive

• What Color Eyes Would Your Children Have? Genetics of human eye color: An interactive introduction.
• Double Helix Game from the Nobel Prize website. Match CATG bases with each other, and other games.