A phenotype (from Greek phainein, meaning "to show", and typos, meaning "type") is the composite of an organism's observable characteristics or traits, such as its morphology, development, biochemical or physiological properties, phenology, behavior, and products of behavior (such as a bird's nest). Richard Dawkins in 1978 and then again in his 1982 book The Extended Phenotype suggested that bird nests and other built structure such as caddis fly larvae cases and beaver dams are "extended phenotypes". A phenotype results from the expression of an organism's genes as well as the influence of environmental factors and the interactions between the two. When two or more clearly different phenotypes exist in the same population of a species, the species is called polymorph.
The genotype of an organism is the inherited instructions it carries within its genome. Not all organisms with the same genotype look or act the same way because appearance and behavior are modified by environmental and developmental conditions. Likewise, not all organisms that look alike necessarily have the same genotype.
This genotype-phenotype distinction was proposed by Wilhelm Johannsen in 1911 to make clear the difference between an organism's heredity and what that heredity produces. The distinction is similar to that proposed by August Weismann, who distinguished between germ plasm (heredity) and somatic cells (the body). The genotype-phenotype distinction should not be confused with Francis Crick's central dogma of molecular biology, which is a statement about the directionality of molecular sequential information flowing from DNA to protein, and not the reverse.
Difficulties in definition
Despite its seemingly straightforward definition, the concept of the phenotype has hidden subtleties. It may seem that anything dependent on the genotype is a phenotype, including molecules such as RNA and proteins. Most molecules and structures coded by the genetic material are not visible in the appearance of an organism, yet they are observable (for example by Western blotting) and are thus part of the phenotype. Human blood groups are an example. It may also seem that this goes beyond the original intentions of the concept with its focus on the (living) organism in itself, meaning that the lowest level of biological organization compatible with the phenotype concept is at the cellular level. Either way, the term phenotype includes traits or characteristics that can be made visible by some technical procedure. A notable extension to this idea is the presence of "organic molecules" or metabolites that are generated by organisms from chemical reactions of enzymes, e.g. vitamins, that can be scored as phenotype. Another extension adds behavior to the phenotype, since behaviors are also observable characteristics. Indeed there is research into the clinical relevance of behavioral phenotypes as they pertain to a range of syndromes. Often, the term "phenotype" is incorrectly used as a shorthand to indicate phenotypical changes observed in mutated organisms (most often in connection with knockout mice).
Phenotypic variation (due to underlying heritable genetic variation) is a fundamental prerequisite for evolution by natural selection. It is the living organism as a whole that contributes (or not) to the next generation, so natural selection affects the genetic structure of a population indirectly via the contribution of phenotypes. Without phenotypic variation, there would be no evolution by natural selection.
The interaction between genotype and phenotype has often been conceptualized by the following relationship:
- genotype (G) + environment (E) → phenotype (P)
A more nuanced version of the relationship is:
- genotype (G) + environment (E) + genotype & environment interactions (GE) → phenotype (P)
Genotypes often have much flexibility in the modification and expression of phenotypes; in many organisms these phenotypes are very different under varying environmental conditions (see ecophenotypic variation). The plant Hieracium umbellatum is found growing in two different habitats in Sweden. One habitat is rocky, sea-side cliffs, where the plants are bushy with broad leaves and expanded inflorescences; the other is among sand dunes where the plants grow prostrate with narrow leaves and compact inflorescences. These habitats alternate along the coast of Sweden and the habitat that the seeds of Hieracium umbellatum land in, determine the phenotype that grows.
An example of random variation in Drosophila flies is the number of ommatidia, which may vary (randomly) between left and right eyes in a single individual as much as they do between different genotypes overall, or between clones raised in different environments.
The concept of phenotype can be extended to variations below the level of the gene that affect an organism's fitness. For example, silent mutations that do not change the corresponding amino acid sequence of a gene may change the frequency of guanine-cytosine base pairs (GC content). These base pairs have a higher thermal stability (melting point, see also DNA-DNA hybridization) than adenine-thymine, a property that might convey, among organisms living in high-temperature environments, a selective advantage on variants enriched in GC content.
The Extended Phenotype
The idea of the phenotype has been generalized by Richard Dawkins in The Extended Phenotype to mean all the effects a gene has on the outside world that may influence its chances of being replicated. These can be effects on the organism in which the gene resides, the environment, or other organisms. The term "extended phenotype" was first coined in 1978.
For instance, a beaver dam might be considered a phenotype of beaver genes, the same way beavers' powerful incisor teeth are phenotype expressions of their genes. Dawkins also cites the effect of an organism on the behavior of another organism (such as the devoted nurturing of a cuckoo by a parent of a different species) as an example of the extended phenotype as well as parasites living inside the body of a host. The first example he used was sporocysts of flukes of the genus Leucochloridium that invade the tentacles of snails where they can be seen conspicuously pulsating through the snail's skin. This change in both color and behavior (infected snails move upwards on vegetation) is suggested to increase predation on the snail by birds and therefore to assist the parasite enter its final host, a bird. The third example of the extended phenotype is "Action at a Distance". This is where genes in one organism affect the behavior of another organism. The examples Dawkins used were genes in orchids affecting orchid bee behavior (to increase pollination), genes in rattlesnakes causing avoidance behavior in other animals, and genes in male peacocks affecting copulatory decisions of peahens.
The smallest unit of replicators is the gene. Replicators cannot be directly selected upon, but they are selected on by their phenotypic effects. These effects are packaged together in organisms. We should think of the replicator as having extended phenotypic effects. These are all of the ways it affects the world, not just the effects the replicators have on the body in which they reside.
Phenome and phenomics
Although a phenotype is the ensemble of observable characteristics displayed by an organism, the word phenome is sometimes used to refer to a collection of traits, while the simultaneous study of such a collection is referred to as phenomics.
- Dawkins 1978 http://onlinelibrary.wiley.com/doi/10.1111/j.1439-0310.1978.tb01823.x/abstract
- Churchill, F.B. (1974). "William Johannsen and the genotype concept". Journal of the History of Biology 7: 5–30. doi:10.1007/BF00179291.
- Johannsen, W. (1911). "The genotype conception of heredity". American Naturalist 45 (531): 129–159. doi:10.1086/279202. JSTOR 2455747.
- O'Brien, Gregory; Yule, William, eds. (1995). Behavioural Phenotypes. Clinics in Developmental Medicine No.138. London: Mac Keith Press. ISBN 1-898683-06-9
- O'Brien, Gregory, ed. (2002). Behavioural Phenotypes in Clinical Practice. London: Mac Keith Press. ISBN 1-898683-27-1. Retrieved 27 September 2010
- Crusio WE (May 2002). "'My mouse has no phenotype'". Genes, Brain and Behavior 1 (2): 71. doi:10.1034/j.1601-183X.2002.10201.x. PMID 12884976. Retrieved 2009-12-29.
- Lewontin, R. C. (November 1970). "The Units of Selection" (PDF). Annual Review of Ecology and Systematics (Palo Alto, CA: Annual Reviews) 1: 1–18. doi:10.1146/annurev.es.01.110170.000245. ISSN 1545-2069. JSTOR 2096764.
- "Botany online: Evolution: The Modern Synthesis - Phenotypic and Genetic Variation; Ecotypes". Retrieved 2009-12-29.
- Dawkins, Richard (1982). The Extended Phenotype. Oxford University. p. 4. ISBN 0-19-288051-9.
- Mahner, M. & Kary, M. (1997). "What exactly are genomes, genotypes and phenotypes? And what about phenomes?". Journal of Theoretical Biology 186: 55–63. doi:10.1006/jtbi.1996.0335.
- Varki, A; Wills, C; Perlmutter, D; Woodruff, D; Gage, F; Moore, J; Semendeferi, K; Bernirschke, K; Katzman, R et al. (1998). "Great Ape Phenome Project?". Science 282 (5387): 239–240. Bibcode:1998Sci...282..239V. doi:10.1126/science.282.5387.239d. PMID 9841385.
- Mouse Phenome Database
- Human Phenotype Ontology
- Europhenome: Access to raw and annotated mouse phenotype data
- "Wilhelm Johannsen's Genotype-Phenotype Distinction" by E. Peirson at the Embryo Project Encyclopedia