Phi X 174: Difference between revisions
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==External links== |
==External links== |
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* [http://www.fermentas.com/techinfo/nucleicacids/mapfx174.htm Description & Restriction Map: PhiX174 DNA] |
<-- BROKEN LINK * [http://www.fermentas.com/techinfo/nucleicacids/mapfx174.htm Description & Restriction Map: PhiX174 DNA] --> |
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* [http://www.ncbi.nlm.nih.gov/nuccore/ |
* [http://www.ncbi.nlm.nih.gov/nuccore/NC_001422 Complete Genome] |
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[[Category:Bacteriophages]] |
[[Category:Bacteriophages]] |
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Revision as of 20:34, 16 May 2011
| Phi X 174 | |
|---|---|
| Virus classification | |
| Group: | Group II (ssDNA)
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| Family: | |
| Genus: | |
| Species: | phi X 174 phage
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The phi X 174 (or phi X) bacteriophage was the first DNA-based genome to be sequenced. This work was completed by Fred Sanger and his team in 1977.[1] In 1962, Walter Fiers had already demonstrated the physical, covalently closed circularity of phi X 174 DNA.[2]
This phage has a very small amount of DNA. Phi X has 11 genes in 5386 bases (it is single stranded) in a circular topology; several of these genes express similar function in two groups. The GC-content is 44% and 95% of nucleotides belong to coding genes.
In 2003, it was reported that the whole genome of phi X 174 had been assembled synthetically from scratch.[3]
Phi X is regularly used as a positive control in DNA sequencing due to its relatively small genome size in comparison to other organisms and the extensive work that has been done on it.
See also
References
- ^ Sanger F, Air GM, Barrell BG, Brown NL, Coulson AR, Fiddes CA, Hutchison CA, Slocombe PM, Smith M (1977-02-24). "Nucleotide sequence of bacteriophage phi X174 DNA". Nature. 265 (5596): 687–695. PMID 870828.
{{cite journal}}: CS1 maint: multiple names: authors list (link) - ^ Fiers W, Sinsheimer RL (1962). "The structure of the DNA of bacteriophage PhiX 174. III. Ultracentrifuge evidence for a ring structure". J. Mol. Biol. 5: 424–434.
- ^ Smith, Hamilton O. (2003-12-23). "Generating a synthetic genome by whole genome assembly: {phi}X174 bacteriophage from synthetic oligonucleotides". Proceedings of the National Academy of Sciences. 100 (26): 15440–15445. doi:10.1073/pnas.2237126100. PMID 14657399. Retrieved 2007-10-08.
{{cite journal}}: Unknown parameter|coauthors=ignored (|author=suggested) (help)
External links
<-- BROKEN LINK * Description & Restriction Map: PhiX174 DNA -->
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