HL60: Difference between revisions
Content deleted Content added
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[cell line]] has been used for laboratory research on how certain kinds of blood cells are formed. |
[[cell line]] has been used for laboratory research on how certain kinds of blood cells are formed. HL-60 proliferates continuously in suspension [[Cell culture|culture]] in nutrie]] and [[antibiotics|antibiotic]] chemicals. The [[doubling time]] is about 36–48 hours. The cell line was derived from a 36-year-old woman with [[acute promyelocytic leukemia]] at the [[National Cancer Institute]].<ref name="pmid288488">{{cite journal |author=Gallagher R, Collins S, Trujillo J, ''et al.'' |title=Characterization of the continuous, differentiating myeloid cell line (HL-60) from a patient with acute promyelocytic leukemia |journal=[[Blood (journal)|Blood]] |volume=54 |issue=3 |pages=713–33 |year =1979 |pmid=288488 |doi= |url=http://www.bloodjournal.org/cgi/pmidlookup?view=long&pmid=288488}}</ref> HL-60 cells are predominantly a [[neutrophil]]ic promyelocyte (precursor).<ref name="pmid288488" /> |
||
Proliferation of HL-60 cells occurs through the [[transferrin]] and [[insulin]] receptors, which are expressed on cell surface. The requirement for insulin and transferrin is absolute, as HL-60 proliferation immediately ceases if either of these compounds is removed from the serum-free culture media.<ref>{{cite journal | doi = 10.1016/0014-4827(80)90296-7 | author = Breitman, T, S. Collins, B. Keene, | year = 1980 | title = Replacement of serum by insulin and transferrin supports growth and differentiation of the human promyelocytic leukemia cell line, HL-60. | journal = Exp. Cell Res | volume = 126 | issue = 2 | pages = 494–498 | pmid = 6988226}}</ref> With this line, spontaneous differentiation to mature [[granulocytes]] can be induced by compounds such as [[dimethyl sulfoxide]] (DMSO), or [[retinoic acid]]. Other compounds like 1,25-dihydroxyvitamin D<sub>3</sub>, [[12-O-tetradecanoylphorbol-13-acetate]] (TPA) and [[GM-CSF]] can induce HL-60 to differentiate to [[monocytic]], [[macrophage]]-like and [[eosinophil]] phenotypes, respectively. |
Proliferation of HL-60 cells occurs through the [[transferrin]] and [[insulin]] receptors, which are expressed on cell surface. The requirement for insulin and transferrin is absolute, as HL-60 proliferation immediately ceases if either of these compounds is removed from the serum-free culture media.<ref>{{cite journal | doi = 10.1016/0014-4827(80)90296-7 | author = Breitman, T, S. Collins, B. Keene, | year = 1980 | title = Replacement of serum by insulin and transferrin supports growth and differentiation of the human promyelocytic leukemia cell line, HL-60. | journal = Exp. Cell Res | volume = 126 | issue = 2 | pages = 494–498 | pmid = 6988226}}</ref> With this line, spontaneous differentiation to mature [[granulocytes]] can be induced by compounds such as [[dimethyl sulfoxide]] (DMSO), or [[retinoic acid]]. Other compounds like 1,25-dihydroxyvitamin D<sub>3</sub>, [[12-O-tetradecanoylphorbol-13-acetate]] (TPA) and [[GM-CSF]] can induce HL-60 to differentiate to [[monocytic]], [[macrophage]]-like and [[eosinophil]] phenotypes, respectively. |
||
Revision as of 06:40, 14 February 2015
cell line has been used for laboratory research on how certain kinds of blood cells are formed. HL-60 proliferates continuously in suspension culture in nutrie]] and antibiotic chemicals. The doubling time is about 36–48 hours. The cell line was derived from a 36-year-old woman with acute promyelocytic leukemia at the National Cancer Institute.[1] HL-60 cells are predominantly a neutrophilic promyelocyte (precursor).[1]
Proliferation of HL-60 cells occurs through the transferrin and insulin receptors, which are expressed on cell surface. The requirement for insulin and transferrin is absolute, as HL-60 proliferation immediately ceases if either of these compounds is removed from the serum-free culture media.[2] With this line, spontaneous differentiation to mature granulocytes can be induced by compounds such as dimethyl sulfoxide (DMSO), or retinoic acid. Other compounds like 1,25-dihydroxyvitamin D3, 12-O-tetradecanoylphorbol-13-acetate (TPA) and GM-CSF can induce HL-60 to differentiate to monocytic, macrophage-like and eosinophil phenotypes, respectively.
The HL-60 cultured cell line provides a continuous source of human cells for studying the molecular events of myeloid differentiation and the effects of physiologic, pharmacologic, and virologic elements on this process. HL-60 cell model was used to study the effect of DNA topoisomerase (topo) IIα and IIβ on differentiation and apoptosis of cells[3] and is especially useful in dielectrophoresis studies,[4] which require an aqueous environment with suspended and round cells.
References
- ^ a b Gallagher R, Collins S, Trujillo J; et al. (1979). "Characterization of the continuous, differentiating myeloid cell line (HL-60) from a patient with acute promyelocytic leukemia". Blood. 54 (3): 713–33. PMID 288488.
{{cite journal}}: Explicit use of et al. in:|author=(help)CS1 maint: multiple names: authors list (link) - ^ Breitman, T, S. Collins, B. Keene, (1980). "Replacement of serum by insulin and transferrin supports growth and differentiation of the human promyelocytic leukemia cell line, HL-60". Exp. Cell Res. 126 (2): 494–498. doi:10.1016/0014-4827(80)90296-7. PMID 6988226.
{{cite journal}}: CS1 maint: extra punctuation (link) CS1 maint: multiple names: authors list (link) - ^ Sugimoto, K, K. Yamada, M. Egashira, Y. yazaki, H. Hirai, A. Kikuchi and K. Oshimi, (1998). "Temporal and Spatial Distribution of DNA Topoisomerase II Alters During Proliferation, Differentiation, and Apoptosis in HL-60 Cells". Blood. 91 (4): 1407–1417. PMID 9454772.
{{cite journal}}: CS1 maint: extra punctuation (link) CS1 maint: multiple names: authors list (link) - ^ Ratanachoo, K., Gascoyne, P.R.C. and Ruchirawat, M. (2002). "Detection of cellular responses to toxicants by dielectrophoresis". Biochim Biophys Acta. 1564 (2): 449–458. doi:10.1016/S0005-2736(02)00494-7. PMC 2726261. PMID 12175928.
{{cite journal}}: CS1 maint: multiple names: authors list (link)
See also
Wikimedia Commons has media related to HL-60 cells.