ATP-independent breakage of single-stranded DNA, followed by passage and rejoining
These enzymes bring about the conversion of one topological isomer of DNA into another. There are two types of DNA topoisomerase; I and II. Both of which are further classified into sub families IA, IB, IIA, and IIB. DNA topoisomerase facilitates a transesterification reaction. Through this reaction DNA topoisomerase is able to break and rejoin the phosphate backbone of DNA.