Talk:Trypsin

Template:Wikiproject MCB Where did Trypsin get its name from?
Trevor H. (UK) 16:35, 27 May 2007 (UTC) says: see http://dictionary.reference.com/search?q=trypsin

Where is trypsin found in the body and what proteins does it break down?

• I thought that trypsins main function was to cleave chymotrypsinogen to chymotrypsin, rather than to hydrolyse proteins. I know it can do either, but I found it a bit od that there is barely a mention of chymotrypsin here.

At what temperature is the enzyme(trypsin) denatured, and is it denatured when it passes through to a different site to where it is not usually found, due to the pH. Is gelatine the only protein that is broken down by trypsin or do different enzymes break it down as well.

--Craig89 10:53, 15 June 2006 (UTC)

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Trypsin is made in the pancreas then secreted in to the intestine

pH cannot denature an enzyme, only stretch the bonds that hold it together. When pH levels are suitable again, the enzyme returns to its normal shape.

User:Alexsheppard11 Biology coursework on enzymes helped me with your questions:P

How about some general info like MW, PI etc. Jasoninkid 16:14, 12 December 2006 (UTC)

pH can denature proteins

FYI, pH can denature proteins (by changing their shape)! Denaturation means that a protein does not retain its shape and does not refer to the lysis of protein amide bonds. Trypsin might be one of those proteins that can retain its shape even at low pH -- I'm not sure if this has been studied. —The preceding unsigned comment was added by 165.140.4.22 (talk) 00:33, 3 April 2007 (UTC).

I can concur on this. --192.38.113.102 12:36, 14 May 2007 (UTC)

PDB names

What are the PDB names for each type of trypsine?
Thanks, Omermar @ 15/6/08
—Preceding unsigned comment added by Omermar (talk • contribs) 14:05, 15 June 2008 (UTC)

Cell Culture

Retracted.— Preceding unsigned comment added by 146.57.129.59 (talk) 20:57, 7 June 2011 (UTC)

Alt symbols

The final box currently reads:

Symbol PRSS3

Alt. symbols PRSS4

Surely this isn't right? If PRSS2 is different from PRSS3, i would rather expect PRSS4 to be something different again. Can anyone confirm that this is the correct alt symbol, or, as i suspect, confirm that PRSS4 needs an entirely separate box. Ta, Abergabe (talk) 13:54, 9 August 2010 (UTC)

PRSS4 was the previous official HUGO gene symbol for PRSS3. In addition Entrez lists PRSS4 as an alias of PRSS3. Because PRSS4 was the previous official gene symbol, one might find reference to it in the older literature. Hence I think PRSS4 is a legitimate alt symbol for PRSS3. Boghog (talk) 14:20, 9 August 2010 (UTC)

Cheers for clearing that up ^_^ Abergabe (talk) 16:25, 9 August 2010 (UTC)

Suggested Merge with Trypsinogen

Trypsinogen is the inactive form of trypsin; seems unecessary to have separate pages for every inactive/active form of protease (and almost no others have pages like this). Barely any info is on trypsinogen page currently, it could easily be included as a subsection on this page insteadKingMunch (talk) 12:02, 19 October 2011 (UTC)

I don't mind having it merged, but I do think it would be more useful to have a separate article. The article can be extended if necessary, since there are a lot data on trypsinogen (crystal structure, variants, etc.) which wouldn't fit into the article on trypsin. Hzh (talk) 17:25, 27 April 2012 (UTC)

en: Merging that isn't a good idea in my opinion. Marek Mazurkiewicz (talk) 00:55, 4 November 2012 (UTC)

eo: Alinomenda artikolo ne estas bonan koncepton. Ja tio ĉi estas malsamaj kemiaj kombinaĵo. Marek Mazurkiewicz (talk) 00:55, 4 November 2012 (UTC)

"Crystal structure" in figure caption

Currently the caption of the figure showing the structure of trypsin reads as "Crystal structure of bovine trypsin", with the "crystal structure" part linking to X-ray crystallography. I'm pretty sure this is not the crystal structure of trypsin (which would tell us how trypsin molecules are ordered with respect to each other in a solid state), but rather its biomacromolecular structure (or something like that, telling us the configuration of a single trypsin molecule) inferred from X-ray crystallography measurements. Or is this some special use of the term in molecular biology? -- Loudandras (talk) 23:03, 17 April 2015 (UTC)

The term "crystal structure" as used in the field of protein crystallography refers to both the ordering of the protein within the crystal (the space group) and the 3D structure of the protein within the asymmetric unit. Both are determined from the same x-ray diffraction experiment. In this context, "crystal structure" means the structure of the protein within a crystal as opposed to for example the protein structure in solution which can be determined by protein NMR. Boghog (talk) 06:45, 18 April 2015 (UTC)

Thanks for the explanation, I had no idea about its use in this context. -- Loudandras (talk) 08:15, 18 April 2015 (UTC)