DyLight Fluor
Color | mass (g/mol) | Absorb (nm) | Emit (nm) | ε (M-1cm-1) | |
---|---|---|---|---|---|
DyLight 488 | green | 1011 | 493 | 518 | 70,000 |
DyLight 549 | yellow | 982 | 550 | 568 | 150,000 |
DyLight 649 | red | 1008 | 646 | 674 | 250,000 |
DyLight 680 | near-IR | 950 | 682 | 715 | 140,000 |
DyLight 800 | near-IR | 1050 | 770 | 794 | 270,000 |
The DyLight Fluor family of fluorescent dyes is produced by Thermo Fisher Scientific. DyLight Dyes are typically used in biotechnology and research applications as biomolecule, cell and tissue labels for fluorescence microscopy, cell biology or molecular biology.
The excitation and emission spectra of the DyLight Fluor series covers much of the visible spectrum and extends into the infrared region, allowing detection using most fluorescence microscopes, as well as infrared imaging systems.
DyLight Fluors are synthesized through sulfonate addition to coumarin, rhodamine, xanthene (such as fluorescein), and cyanine dyes. Sulfonation makes DyLight Dyes negatively charged and hydrophilic. DyLight Fluors are commercially available as reactive succinimidyl-esters for labeling proteins through lysine residues, and as maleimide derivatives for labeling proteins through cysteine residues.
Historically, fluors such as fluorescein, rhodamine, Cy3 and Cy5 have been used in a wide variety of applications. These dyes have major limitations for use in microscopy and other applications that require exposure to a laser light source, because they photobleach quickly. DyLight Fluors have comparable excitation and emission spectra and are generally more photostable, brighter, and less pH-sensitive.
The Alexa Fluor dyes are a similar line of fluorescent dyes from Invitrogen that offer comparable alternatives to the DyLight Fluors.