2 base encoding

2 Base Encoding is a new DNA sequencing technology that has been developed by Applied Biosystems. It is one of several next-generation sequencing technologies that are now being developed and commercialized. These technologies generate hundreds of thousands of small sequence reads at one time. Well-known examples include 454 pyrosequencing (introduced 2005), Solexa system (introduced 2006) and the 2-base encoding sequencing (introduced 2007-2008). These methods have reduced the cost from almost $0.01/base in 2004 to near $0.0001/base in 2006 and increased the sequencing machine capacity from 1,000,000 base/machine/day in 2004 to more than 100,000,000 base/machine/day in 2006.

General features

The general steps in all of these next-generation sequencing techniques include:

1- Random fragmentation of genomic DNA

2- Immobilization of single DNA fragments on a solid support like a bead or planar solid surface

3- Amplification of DNA fragments on the solid surface using PCR and making polymerase colonies

4- Sequencing and subsequent in situ interrogation after each cycle using fluorescence scanning or chemiluminescence .

In 2005 Shendure et al. used a sequencing procedure using multiple cycles of ligation of fluorescent labled 9-mer probes which distinguish the central base. In each cycle the sequence of every fifth base is recognized. This process is repeated using different primers to sequence the remaining four bases in each gap . The most recent next-generation sequencing technology which is called 2-base encoding or SOLiD (Sequencing by Oligonucleotide Ligation and Detection) technology has been developed by Applied Biosystem and will be commercially available in 2008. Similar to Shendure et al. and despite other two next-generation sequencing technologies, 2-base encoding is based on ligation sequencing rather than sequencing by synthesis. However, its fundamental difference to previously used 9-mer probes with distinguished central base is taking advantage of fluorescent labeled 8-mer probes with distinguished the 2 central bases.

How it works

The SOLiD Sequencing System uses probes with dual base encoding.

The underlying chemistry is summarized in the following steps:

- Step1, preparing a library: This step begins with shearing the genomic DNA into small fragments. Then two different adapters are added (for example A1 and A2). The resultant library contains template DNA fragments, which are tagged with one adapter at each end.

- Step2, emulsion PCR: in this step the emulsion (water in oil emulsion) PCR reaction is performed using DNA fragments from library, two primers (P1 and P2) complement to the previously used adapters (P1 with A1 and P2 with A2), other PCR reaction components and beads coupled with one of the primers (e.g. P1). The aim is locating one DNA template and one bead into a single emulsion droplet.

In each droplet, DNA template anneals to the P1-coupled bead from its A1 side. Then DNA polymerase will extend from P1 to make the complementary sequence, which eventually results in a bead enriched with PCR products from a single template. After PCR reaction, templates are denatured and disassociate from the beads.

- Step3, bead enrichment: in practice, only 30% of beads have target DNA. To enrich the number of these beads, large polystyrene beads coated with A2 are added to the solution. Thus, any bead containing the extended products will bind polystyrene bead through its P2 end. The resultant complex will be separated from untargeted beads, and melt of to dissociate the targeted beads from polystyrene. This step can increases the throughput of this system from 30% before enrichment to 80% after enrichment.

After enrichment, the 3’-end of products (P2 end) will be modified which makes them capable of covalent bonding in the next step. Therefore, the products of this step are DNA-coupled beads with 3’-modification of each DNA strand.

- Step4, bead deposition: In this step, products of the last step are deposited onto a glass slide. Beads attach to the glass surface randomly through covalent bonds of the 3’-modified beads and the glass.

- Step5, sequencing reaction

File:Database-2 base encoding.png

Figure 1- Schematic feature of four different probes used in 2 base encoding.

File:2 Base Encoding.png

Figure 2- Schematic feature of how 2-base encoding system works. Each base pair in the sequence is read twice and enables this system to minimizes the rate of errors.

For decoding the colors we must first know that each single color indicates two bases and second, we need to know one of the bases in the sequence.

Advantages

-Accuracy: each base in this sequencing method is interrogated and interpreted twice. This can increase the accuracy of the system to more than 99.94% which is higher than other systems.

-Detection of SNPs and other small changes: One of the main advantages of this system is its ease in detection of single nucleotide polymorphisms (SNPs) as well as other small alterations in the template sequence. Changing the color of two adjacent bases is characteristic for SNPs. The detection of other alterations is summarized in Figure 2.

- Detection of errors: as discussed earlier, each base in this system is recognized by two colors and alteration in any single base will result in change in two colors. Therefore, while alteration in two or more than two colors demonstrate a real change in our sequence, just one color change indicates an error and should not be considered as a change (Figure 2).

Conclusion

Next-generation sequencing method	Number of bp/run	Duration of each run	Read length (bp)	Cost	Accuracy
454	100 million	7.5 hours	up to 250	1:10-20 of Sanger sequencing	> 99.5%
Solexa (1G)	1000 million	3 days	up to 50	1:20-30 of 454 sequencing	> 99.93%
2 base encoding	2000 million	4 days	up to 35	1:20-30 of 454 sequencing	> 99.94%

External Links

• http://www.454.com
• http://www.solexa.com