Resazurin

Resazurin
Names
	IUPAC name 7-hydroxy-10-oxidophenoxazin-10-ium-3-one
Identifiers
CAS Number	550-82-3;
3D model (JSmol)	Interactive image;
ECHA InfoCard	100.008.171
PubChem CID	11077;
CompTox Dashboard (EPA)	DTXSID4060280 ;
	SMILES C1=CC2=C(C=C1O)OC3=CC(=O)C=CC3=[N+]2[O-];
Properties
Chemical formula	C12H7NO4
Molar mass	229.191 g·mol−1
Solubility in water	soluble
	Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). Infobox references

Resazurin (7-Hydroxy-3H-phenoxazin-3-one 10-oxide) is a blue dye used mainly as an oxidation-reduction indicator in the resazurin test for bacteria. It was first used to quantify bacterial content in milk by Pesch and Simmert in 1929.^[1] It is also used as an indicator for cell viability in mammalian cell cultures ^[2]. Blue colored Resazurin is reduced to pink colored, fluorescent compound Resorufin in the presence of live cells. Usually it is available commercial as the sodium salt. It is available under the trade name Alamar Blue.

Resazurin solution is the substance with the high value of Kreft's dichromaticity index.^[3] This means that it has the large change in color hue, when the thickness of concentration of observed sample increases or decreases.

References

^ Pesch K. L., Simmert U. (1929). Milchw. Forsch. 8, 551
^ Anoopkumar-Dukie (2005). "Resazurin assay of radiation response in cultured cells[1]". Br J Radiol. 78: 945–947. doi:10.1259/bjr/54004230. {{cite journal}}: External link in |title= (help)
^ Kreft S, Kreft M. (2009). "Quantification of dichromatism: a characteristic of color in transparent materials[2]". Journal of the Optical Society of America A. 26: 1576–1581. doi:10.1364/JOSAA.26.001576. {{cite journal}}: External link in |title= (help)

[1] Pesch K. L., Simmert U. (1929). Milchw. Forsch. 8, 551

[2] Anoopkumar-Dukie (2005). "Resazurin assay of radiation response in cultured cells[1]". Br J Radiol. 78: 945–947. doi:10.1259/bjr/54004230. {{cite journal}}: External link in |title= (help)

[3] Kreft S, Kreft M. (2009). "Quantification of dichromatism: a characteristic of color in transparent materials[2]". Journal of the Optical Society of America A. 26: 1576–1581. doi:10.1364/JOSAA.26.001576. {{cite journal}}: External link in |title= (help)

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