RNA extraction

RNA extraction is the purification of RNA from biological samples. This procedure is complicated by the ubiquitous presence of ribonuclease enzymes in cells and tissues, which can rapidly degrade RNA.^[1] Several methods are used in molecular biology to isolate RNA from samples, the most common of these is Guanidinium thiocyanate-phenol-chloroform extraction.^[2]^[3]

RNA extraction in liquid nitrogen, commonly using a mortar and pestle, or specialised motorized grinding device [cell lysis device] is also useful in preventing ribonuclease activity.

References

^ Peirson SN, Butler JN (2007). "RNA extraction from mammalian tissues". Methods Mol. Biol. Methods in Molecular Biology. 362: 315–27. doi:10.1007/978-1-59745-257-1_22. ISBN 978-1-58829-417-3. PMID 17417019.
^ Chomczynski P, Sacchi N (2006). "The single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction: twenty-something years on". Nat Protoc. 1 (2): 581–5. doi:10.1038/nprot.2006.83. PMID 17406285.
^ Bird IM (2005). "Extraction of RNA from cells and tissue". Methods Mol. Med. 108: 139–48. PMID 16028681.

[1] Peirson SN, Butler JN (2007). "RNA extraction from mammalian tissues". Methods Mol. Biol. Methods in Molecular Biology. 362: 315–27. doi:10.1007/978-1-59745-257-1_22. ISBN 978-1-58829-417-3. PMID 17417019.

[2] Chomczynski P, Sacchi N (2006). "The single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction: twenty-something years on". Nat Protoc. 1 (2): 581–5. doi:10.1038/nprot.2006.83. PMID 17406285.

[3] Bird IM (2005). "Extraction of RNA from cells and tissue". Methods Mol. Med. 108: 139–48. PMID 16028681.

[1]

[2]

[3]

See also

References