It is the most widely used "genetic background" for genetically modified mice for use as models of human disease. They are the most widely used and best-selling mouse strain, due to the availability of congenic strains, easy breeding, and robustness.
Appearance and behavior
C57BL/6 mice have a dark brown, nearly black coat. They are more sensitive to noise and odours and are more likely to bite than the more docile laboratory strains such as BALB/c. They are good breeders.
Group-housed B6 female mice display barbering behavior, in which the dominant mouse in a cage selectively removes hair from its subordinate cage mates. Mice that have been barbered have large bald patches on their bodies, commonly around the head, snout, and shoulders, although barbering may appear anywhere on the body. Both hair and whiskers may be removed.
C57BL/6 has many unusual characteristics that make it useful for some work and inappropriate for other: It is unusually sensitive to pain and to cold, and analgesic medications are less effective in it. Unlike most mouse strains, it drinks alcoholic beverages voluntarily. It is more susceptible than average to morphine addiction, atherosclerosis, and age-related hearing loss.
The dark coat make the mouse strain convenient for creating transgenic mice: it is crossed with a light-furred 129 mouse, and the desirable crosses can be easily identified by their mixed coat colors.
There now exist colonies of mice derived from the original C57BL/6 colony that have been bred in isolation from one another for many hundreds of generations. Owing to genetic drift these colonies differ widely from one another (and, it goes without saying, from the original mice isolated at the Bussey Institute). Responsible scientists, including those at accredited repositories, are careful to point out this fact and take pains to distinguish sublines such as C57BL/6J (the established subline at The Jackson Laboratory) from C57BL/6N, etc. But even within these sublines, the potential for drift exists in colonies maintained by individual laboratories who do not have a systematic practice of reestablishing breeders from a centralized, vetted stock.
By far the most popular laboratory rodent, the C57BL/6 mouse accounts for half to five-sixths of all rodents shipped to research laboratories from American suppliers. Its overwhelming popularity is due largely to inertia: it has been widely used and widely studied, and therefore it is used even more.
- Engber, Daniel (17 November 2011). "The Trouble With Black-6: A tiny alcoholic takes over the lab". Slate.com.
- Connor, A.B. (2006). "Aurora's Guide to Mouse Colony Management" (PDF). Cell Migration Gateway. CMC Activity Center. Retrieved 19 December 2013.
- Sarna, JR; Dyck, RH; Whishaw, IQ (February 2000). "The Dalila effect: C57BL6 mice barber whiskers by plucking". Behavioural Brain Research. 108 (1): 39–45. doi:10.1016/S0166-4328(99)00137-0. PMID 10680755.
- Mogil JS, Wilson SG, Bon K, et al. (March 1999). "Heritability of nociception I: responses of 11 inbred mouse strains on 12 measures of nociception". Pain. 80 (1–2): 67–82. doi:10.1016/s0304-3959(98)00197-3. PMID 10204719.
- Willott, James F. (April 2013). "Audition". In Crusio, Wim E.; Sluyter, Frans; Gerlai, Robert T.; Pietropaolo, Susanna (eds.). Behavioral Genetics of the Mouse: Genetics of Behavioral Phenotypes. Cambridge Handbooks in Behavioral Genetics. Cambridge, United Kingdom: Cambridge University Press. pp. 36–44. ISBN 978-1-107-03481-5.
- Köntgen, F.; Süss, G.; Stewart, C.; Steinmetz, M.; Bluethmann, H. (5 August 1993). "Targeted disruption of the MHC class II Aa gene in C57BL/6 mice" (PDF). International Immunology. 5 (8): 957–964. doi:10.1093/intimm/5.8.957. ISSN 0953-8178. PMID 8398989.
- Zak, Anatoly. "Bion (12KSM) satellite". RussianSpaceWeb. Retrieved 19 April 2013.
- "LIFTOFF: FALCON 9 AND DRAGON BEGIN CRS-6 MISSION TO RESUPPLY THE SPACE STATION". Retrieved 14 April 2015.
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