49th parallel may refer to:
- 49th parallel north, a circle of latitude in the Northern Hemisphere
- 49th parallel south, a circle of latitude in the Southern Hemisphere
- 49th Parallel (film), a 1941 Canadian and British film
- Canada–United States border, sometimes referred to as the "49th parallel" due to much of it following the 49th parallel north
- Hymns of the 49th Parallel, a music album by K.D. Lang
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